The highly sensitive impedimetric biosensor in label free approach for hepatitis B virus DNA detection based on tellurium doped ZnO nanowires

Abstract

The highly sensitive impedimetric biosensor in label free approach for hepatitis B virus DNA (HPV DNA) detection based on tellurium doped ZnO nanowires was fabricated. The NWs were grown by hybrid thin film oxidation in the physical vapor deposition (PVD) mechanism. The morphology characterization of the synthesized NWs was performed by field emission scanning electron microscopy (FESEM) and the images demonstrated that the diameter and the length of the materialized NWs were around 50 nm and several micrometers, respectively. The high-resolution transmission electron microscopy (HRTEM) image indicated that the fabricated NWs were crystalline and their phase characterization was validated by the X-ray diffraction pattern (XRD pattern). The single-stranded DNA (ss DNA) probe was immobilized on the surface of the Te-ZnO NWs. The electrochemical impedance spectra (EIS) measurements showed high response sensitivity after hybridization with complementary oligonucleotides. The biosensor could distinguish complementary target from non-complementary and mismatch oligonucleotides. The HBV biosensor could respond to complementary target in the concentrations range from 1 pM to 1 μM. The limit of detection (LOD) of the biosensor was 0.1 pM. The stability of the HBV DNA biosensor was investigated and biosensor could show 95% of its initial responses after 8 weeks maintenance.

ZnOTe Compounds Grown by DC-Magnetron Co-Sputtering

“…From XRD results, we conclude that it is possible to obtain Te doping ZnO for the Te concentrations used in this work or lower by using the DC cosputtering magnetron technique while maintaining control of the sample compositions. As previously reported [26,27], these results could have relevant consequences for the band structure of the obtained compounds.…”

“…[24] published an interesting paper dealing with different parameters affecting the growth of ZnTeO alloys and their influence in band gap modulation. In the field of Sputtering deposition techniques, RF magnetron sputtering is the most common [25,26], particularly with ZnTe as the unique target, to obtain ZnTeO alloys [27]. Recently, RF magnetron sputtering using two independent targets of ZnTe and ZnO was also used [28].…”

Electrochemical Impedance Spectroscopy (EIS): Principles, Construction, and Biosensing Applications

“…The approach exhibited excellent electrode tissue impedance suitable for recording electrocardiogram signals without any wet-gel adhesives [ 79 ]. Moreover, tellurium doped zinc oxide (Te-ZnO NWs) nanowires were used for highly sensitive impedimetric DNA sensors in a label-free approach for hepatitis B virus (HBV) detection [ 80 ]. The HBV-DNA sensor responded to the complementary target in a concentration range from 1 pM to 1 μM, with the detection limit of 0.1 pM.…”

Nanomaterials for virus sensing and tracking

“…375 In tellurium doped ZnO nanowires (diameter: 50 nm), tellurium promotes the donor-acceptor emissions and simultaneously passivates the essential vacancy parameters due to isoelectronic doping. 376 When such nanowires are doped with molybdenum, charge transfer is promoted and vacant sites are generated for adsorbing DNA. 377 These nanowires show higher precision in comparison to PCR by demonstrating a higher sensitivity against false negatives.…”