The highly expressed COL4A1 genes contributes to the proliferation and migration of the invasive ductal carcinomas

Jin, Rongzhong; Shen, Jia; Zhang, Tiancheng; Liu, Qiliang; Liao, Caihua; Ma, Hailin; Li, Sijing; Yu, Zhaoxia

doi:10.18632/oncotarget.17345

Cited by 39 publications

(41 citation statements)

References 36 publications

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“…Recently, COL4A1 was reported to be markedly increased and regulated diverse cellular functions such as metabolism, proliferation and protein synthesis through the activation of the PI3K/AKT pathway in urothelial carcinoma . It has also been demonstrated that highly expressed COL4A1 contributed to ductal carcinoma cell proliferation and migration by activating the extracellular matrix receptor in breast tissues . But the function of COL4A1 in gastric carcinoma has not hitherto been well understood.…”

Section: Discussionmentioning

confidence: 99%

“…33 It has also been demonstrated that highly expressed COL4A1 contributed to ductal carcinoma cell proliferation and migration by activating the extracellular matrix receptor in breast tissues. 34 But the function of COL4A1 in gastric carcinoma has not hitherto been well understood. Thus far, this study reported that COL4A1 was overexpressed and associated with a poor prognosis in gastric carcinoma by bioinformatics analysis.…”

Section: Discussionmentioning

confidence: 99%

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Bioinformatics analysis suggests that COL4A1 may play an important role in gastric carcinoma recurrence

Wang

Chang

et al. 2019

J of Digest Diseases

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Objective Cancer recurrence is a complicated problem for clinicians that contributes to poor prognosis. This study aimed to use advanced gastric carcinoma genes profiles to predict increased risk of cancer recurrence in order to identify patients in need of adjuvant therapy for prognosis improvement. Methods Differentially expressed genes were identified for advanced gastric carcinoma by analyzing the GSE2685 from the Gene Expression Omnibus database (GEO) using R package. The candidate genes were then obtained by gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, protein‐protein interaction analysis and survival analysis. Logistic regression analysis was performed to determine the relationship between candidate genes and the recurrence of gastric carcinoma. Results Collagen type IV alpha 1 (COL4A1) was overexpressed in gastric carcinoma tissue by analyzing the GSE2685 gene expression profiles from the Gene Expression Omnibus database. COL4A1 was also overexpressed in gastric carcinoma tissue from the Cancer Genome Atlas dataset and further determined that higher COL4A1 expression led to poorer overall survival. A univariate analysis suggested that COL4A1 was strongly correlated with T stage and gastric carcinoma recurrence (P = 0.014 and 0.041, respectively). Moreover, a multiple logistic regression analysis indicated that COL4A1 was significantly associated with gastric carcinoma recurrence (hazard ratio 1.605, 95% confidence interval 1.063‐2.677, P = 0.008). Conclusions COL4A1 may promote gastric carcinoma recurrence and could be used as a therapeutic target for gastric carcinoma recurrence.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Bioinformatics analysis suggests that COL4A1 may play an important role in gastric carcinoma recurrence

Wang

Chang

et al. 2019

J of Digest Diseases

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“…Cells were exposed to drug treatment for indicated time and lysed in RIPA buffer as detailed in previous report [32]. Lysate was mixed with sample loading buffer and heated at 100oC for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Magnolol Inhibits the Growth of Non-Small Cell Lung Cancer via Inhibiting Microtubule Polymerization

Shen

Zhang

et al. 2017

Cell Physiol Biochem

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Background: The tubulin/microtubule system, which is an integral component of the cytoskeleton, plays an essential role in mitosis. Targeting mitotic progression by disturbing microtubule dynamics is a rational strategy for cancer treatment. Methods: Microtubule polymerization assay was performed to examine the effect of Magnolol (a novel natural phenolic compound isolated from Magnolia obovata) on cellular microtubule polymerization in human non-small cell lung cancer (NSCLC) cells. Cell cycle analysis, mitotic index assay, cell proliferation assay, colony formation assay, western blotting analysis of cell cycle regulators, Annexin V-FITC/PI staining, and live/dead viability staining were carried out to investigate the Magnolol’s inhibitory effect on proliferation and viability of NSCLS cells in vitro. Xenograft model of human A549 NSCLC tumor was used to determine the Magnolol’s efficacy in vivo. Results: Magnolol treatment effectively inhibited cell proliferation and colony formation of NSCLC cells. Further study proved that Magnolol induced the mitotic phase arrest and inhibited G2/M progression in a dose-dependent manner, which were mechanistically associated with expression alteration of a series of cell cycle regulators. Furthermore, Magnolol treatment disrupted the cellular microtubule organization via inhibiting the polymerization of microtubule. We also found treatment with NSCLC cells with Magnolol resulted in apoptosis activation through a p53-independent pathway, and autophgy induction via down-regulation of the Akt/mTOR pathway. Finally, Magnolol treatment significantly suppressed the NSCLC tumor growth in mouse xenograft model in vivo. Conclusion: These findings identify Magnolol as a promising candidate with anti-microtubule polymerization activity for NSCLC treatment.

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“…4A and B). 7,14,43,46 In contrast, nonfluorescent samples expressed genes associated with mature cells of the central nervous system such as MOBP, MAL, and OPALIN ( Fig. 4A and C).…”

Section: Intraoperative 5-ala-induced Fluorescence In Gbm Specimens Imentioning

confidence: 99%

Characterizing the heterogeneity in 5-aminolevulinic acid–induced fluorescence in glioblastoma

Bonnin

Havrda

Lee

et al. 2020

Journal of Neurosurgery

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OBJECTIVE5-aminolevulinic acid (5-ALA)–induced protoporphyrin IX (PpIX) fluorescence is an effective surgical adjunct for the intraoperative identification of tumor tissue during resection of high-grade gliomas. The use of 5-ALA-induced PpIX fluorescence in glioblastoma (GBM) has been shown to double the extent of gross-total resection and 6-month progression-free survival. The heterogeneity of 5-ALA-induced PpIX fluorescence observed during surgery presents a technical and diagnostic challenge when utilizing this tool intraoperatively. While some regions show bright fluorescence after 5-ALA administration, other regions do not, despite that both regions of the tumor may be histopathologically indistinguishable. The authors examined the biological basis of this heterogeneity using computational methods.METHODSThe authors collected both fluorescent and nonfluorescent GBM specimens from a total of 14 patients undergoing surgery and examined their gene expression profiles.RESULTSIn this study, the authors found that the gene expression patterns characterizing fluorescent and nonfluorescent GBM surgical specimens were profoundly different and were associated with distinct cellular functions and different biological pathways. Nonfluorescent tumor tissue tended to resemble the neural subtype of GBM; meanwhile, fluorescent tumor tissue did not exhibit a prominent pattern corresponding to known subtypes of GBM. Consistent with this observation, neural GBM samples from The Cancer Genome Atlas database exhibited a significantly lower fluorescence score than nonneural GBM samples as determined by a fluorescence gene signature developed by the authors.CONCLUSIONSThese results provide a greater understanding regarding the biological basis of differential fluorescence observed intraoperatively and can provide a basis to identify novel strategies to maximize the effectiveness of fluorescence agents.

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The highly expressed COL4A1 genes contributes to the proliferation and migration of the invasive ductal carcinomas

Cited by 39 publications

References 36 publications

Bioinformatics analysis suggests that COL4A1 may play an important role in gastric carcinoma recurrence

Bioinformatics analysis suggests that COL4A1 may play an important role in gastric carcinoma recurrence

Magnolol Inhibits the Growth of Non-Small Cell Lung Cancer via Inhibiting Microtubule Polymerization

Characterizing the heterogeneity in 5-aminolevulinic acid–induced fluorescence in glioblastoma

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