The highly conserved spermatophyte cell wall DUF642 protein family: Phylogeny and first evidence of interaction with cell wall polysaccharides in vitro

Vázquez‐Lobo, Alejandra; Roujol, David; Zúñiga-Sánchez, Esther; Albenne, Cècile; Gamboa‐deBuen, Alicia; Jamet, Élisabeth

doi:10.1016/j.ympev.2012.02.001

Cited by 36 publications

(44 citation statements)

References 49 publications

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“…There are also a number of proteins that are connected by a domain of unknown function (DUF) deeply involved in cell wall metabolism (lower left in Fig. 6) (e.g., Garza-Caligaris et al, 2012;Vazquez-Lobo et al, 2012).…”

Section: Functional Category Comparisons Among the Differentially Rmentioning

confidence: 99%

Spaceflight Induces Specific Alterations in the Proteomes of Arabidopsis

Ferl

Koh²,

Denison³

et al. 2015

Astrobiology

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Life in spaceflight demonstrates remarkable acclimation processes within the specialized habitats of vehicles subjected to the myriad of unique environmental issues associated with orbital trajectories. To examine the response processes that occur in plants in space, leaves and roots from Arabidopsis (Arabidopsis thaliana) seedlings from three GFP reporter lines that were grown from seed for 12 days on the International Space Station and preserved on orbit in RNAlater were returned to Earth and analyzed by using iTRAQ broad-scale proteomics procedures. Using stringent criteria, we identified over 1500 proteins, which included 1167 leaf proteins and 1150 root proteins we were able to accurately quantify. Quantification revealed 256 leaf proteins and 358 root proteins that showed statistically significant differential abundance in the spaceflight samples compared to ground controls, with few proteins differentially regulated in common between leaves and roots. This indicates that there are measurable proteomics responses to spaceflight and that the responses are organspecific. These proteomics data were compared with transcriptome data from similar spaceflight samples, showing that there is a positive but limited relationship between transcriptome and proteome regulation of the overall spaceflight responses of plants. These results are discussed in terms of emergence understanding of plant responses to spaceflight particularly with regard to cell wall remodeling, as well as in the context of deriving multiple omics data sets from a single on-orbit preservation and operations approach.

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Section: Functional Category Comparisons Among the Differentially Rmentioning

confidence: 99%

Spaceflight Induces Specific Alterations in the Proteomes of Arabidopsis

Ferl

Koh²,

Denison³

et al. 2015

Astrobiology

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“…They may modulate the activity of PMEs, which are more abundant in the outer layers than in the endosperm, suggesting that their function could be related to the regulation of HG modifications by interacting with the catalytic domain of PME. Their interaction with cellulose was also demonstrated in vitro but no functional studies yet confirm these interactions [60]. Pectin acetylesterases (PAEs) and AXEs were almost only found in outer layers.…”

Section: Discussionmentioning

confidence: 99%

Cell Wall Proteome of Wheat Grain Endosperm and Outer Layers at Two Key Stages of Early Development

Cherkaoui

Lollier

Geairon

et al. 2019

IJMS

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The cell wall is an important compartment in grain cells that fulfills both structural and functional roles. It has a dynamic structure that is constantly modified during development and in response to biotic and abiotic stresses. Non-structural cell wall proteins (CWPs) are key players in the remodeling of the cell wall during events that punctuate the plant life. Here, a subcellular and quantitative proteomic approach was carried out to identify CWPs possibly involved in changes in cell wall metabolism at two key stages of wheat grain development: the end of the cellularization step and the beginning of storage accumulation. Endosperm and outer layers of wheat grain were analyzed separately as they have different origins (maternal and seed) and functions in grains. Altogether, 734 proteins with predicted signal peptides were identified (CWPs). Functional annotation of CWPs pointed out a large number of proteins potentially involved in cell wall polysaccharide remodeling. In the grain outer layers, numerous proteins involved in cutin formation or lignin polymerization were found, while an unexpected abundance of proteins annotated as plant invertase/pectin methyl esterase inhibitors were identified in the endosperm. In addition, numerous CWPs were accumulating in the endosperm at the grain filling stage, thus revealing strong metabolic activities in the cell wall during endosperm cell differentiation, while protein accumulation was more intense at the earlier stage of development in outer layers. Altogether, our work gives important information on cell wall metabolism during early grain development in both parts of the grain, namely the endosperm and outer layers. The wheat cell wall proteome is the largest cell wall proteome of a monocot species found so far.

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“…The roles of proteins with domains of unknown function PUFs are mostly unknown, but this functional class ofers potential for future research. "mong PUFs, the DUF642 proteins have been shown to interact with cellulose in vitro [51]. They could also be involved in pectin methylesteriication or in defence [52,53].…”

Section: the Plant Cell Wall Proteomementioning

confidence: 99%

Cell Wall Proteomics as a Means to Identify Target Genes to Improve Second‐Generation Biofuel Production

Calderan-Rodrigues¹,

Fonseca²,

Labate³

et al. 2017

Frontiers in Bioenergy and Biofuels

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Second-generation biofuels "2G generally uses residues composed of lignocellulosic materials to produce renewable energy potentially up to 50% , without increasing the planted areas. However, the high cost of enzymes required for cell wall disassembly prior to the sacchariication makes the "2G production more expensive yet, compared to the irst-generation biofuels. Designing plants with less lignin, a barrier to "2G production, or facilitating cell wall disassembly by searching for the plant mechanisms can be the way to obtain "2G feasibility. Therewith, plant cell wall proteomics provides valuable information concerning the main cell wall proteins CWPs involved in its biosynthesis and rearrangements. Essentially, two plants of the grass family have been studied: sugarcane as a crop amenable to second-generation ethanol E2G production and Brachypodium distachyon as a model plant amenable to genetic transformation. Cell wall proteomics has allowed the identiication of numerous CWPs as well as their ine proiling in diferent organs and at various developmental stages. Proteins acting on carbohydrates, mostly glycosyl hydrolases, and oxidoreductases, including class III peroxidases and laccases, can be highlighted. "oth kinds of CWPs are assumed to contribute to the remodelling of cell wall polysaccharides by enzymatic or nonenzymatic mechanisms. CWPs present in growing organs could also be atractive candidates since they greatly contribute to cell wall plasticity.Keywords: Brachypodium distachyon, cell wall protein, grass, second generation ethanol, sugarcane © 2017 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.© 2017 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. . IntroductionSecond-generation biofuels "2G are a promising renewable alternative to supply energy demand of fossil fuels worldwide, whose advantage is mostly due to the lower emission of greenhouse gases and the possibility to increase the production without widening the planted area. However, we are still far from producing "2G at an economically competitive way and reasonable amount to replace fossil fuels. "2G uses lignocellulosic material as substrates. Since sugarcane has been considered one of the best crops to produce bioethanol, its bagasse and straw have been studied as one of the main complementary sources of C 6 and C 5 sugars for "2G. One of the main constrains to its economic feasibility relies on the rate of success of the enzymatic sacchariication enabling the conversion of the plant cell wall sugars into bioethanol [1]. Sacchariication of the cell wall ...

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The highly conserved spermatophyte cell wall DUF642 protein family: Phylogeny and first evidence of interaction with cell wall polysaccharides in vitro

Cited by 36 publications

References 49 publications

Spaceflight Induces Specific Alterations in the Proteomes of Arabidopsis

Spaceflight Induces Specific Alterations in the Proteomes of Arabidopsis

Cell Wall Proteome of Wheat Grain Endosperm and Outer Layers at Two Key Stages of Early Development

Cell Wall Proteomics as a Means to Identify Target Genes to Improve Second‐Generation Biofuel Production

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