The herbicide atrazine affects sperm quality and the expression of antioxidant and spermatogenesis genes in zebrafish testes

Bautista, Felix Esteban Airahuacho; Varela, Antônio Sérgio; Corcini, Carine Dahl; Acosta, Izani Bonel; Caldas, Sergiane Souza; Prímel, Ednei Gilberto; Zanette, Juliano

doi:10.1016/j.cbpc.2018.02.003

Cited by 29 publications

(10 citation statements)

References 42 publications

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“…For example, ATZ seriously affects liver metabolism and immune responses in silver catfish (Kirsten et al, ; Kreutz, Barcellos, dos Santos, Pivato, & Zanatta, ), crayfish (Stara, Kouba, & Velisek, ) and common carp (Cui et al, ; Khalil, Reda, & Awad, ). Exposure to ATZ may also cause reproductive failure and reduce spermatogenesis in zebrafish (Bautista et al, ). Moreover, ATZ prompt shifts in liver and spleen (Oliveira et al, ).…”

Section: Introductionmentioning

confidence: 99%

Alleviating effects of β‐glucan inOreochromis niloticuson growth performance, immune reactions, antioxidant, transcriptomics disorders and resistance toAeromonas sobriacaused by atrazine

2020

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Three hundred Oreochromis niloticus were divided into five groups; the control fish (CNT) were fed a basal diet, beta glucan (βG) group was fed 0.5 g/kg−1 βG, atrazine (ATZ) group was exposed to 1/5 96‐hr LC50 (1.39 mg/L) ATZ, the (βG/ATZ) group was fed βG while being exposed to ATZ, and the (βG then ATZ) group was supplemented with βG for fifteen days before exposed to ATZ. ATZ exposure caused a decline in growth that was ameliorated by βG. ATZ reduced the levels of total and different types of leucocytes. Additionally, ATZ exposure caused reductions in total proteins, globulins, α 1‐globulin, α 2‐globulin, ɤ‐globulin, immunoglobulin M, lysozymes, superoxide dismutase, nitric oxide and catalase but increases in hepatic transaminases and malondialdehyde without any variations in albumin and β‐globulin. Exposure to ATZ also resulted in a rise in the mRNA level of IL‐8. In contrast, expression of IgM, SOD and CAT were decreased in the tilapias exposed to ATZ. Exposure to ATZ increases the susceptibility response to Aeromonas sobria challenge, as indicated by an increase in cumulative mortality post‐challenge. Supplementation with βG fifteen days before (βG then ATZ group), counteracted the adverse effects of ATZ on the immune, biochemical and antioxidants values, though only slight alleviation was observed with simultaneous treatment (βG/ATZ group). Our results established that ATZ has adverse impacts on immune responses, antioxidant equilibrium and its related genes. While, supplementation with βG before exposure to ATZ may be valuable for counteracting the possible damage caused by ATZ water pollution than its simultaneous treatment with ATZ.

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Section: Introductionmentioning

confidence: 99%

Alleviating effects of β‐glucan inOreochromis niloticuson growth performance, immune reactions, antioxidant, transcriptomics disorders and resistance toAeromonas sobriacaused by atrazine

2020

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“…These include high synteny with the human genome, optical clarity of embryos, highly developed immune system, rapid life cycle, and ease of genetic modification, among others. 64 In Brazil, the zebrafish has been used to characterize the mechanism of action and toxicity of several chemicals, such as metals, [65][66][67] pesticides, [68][69][70] pharmaceutical compounds, [71][72][73] and nanomaterial. [74][75][76] Pharmacology & Toxicology is the second largest area researched in Brazil, and accounts for 16.9% of the articles published up to 2019, contrary to what is observed worldwide, where more developmental biology, cellular biology, molecular biology, biochemistry, and genetics studies have been published.…”

Section: Research Areasmentioning

confidence: 99%

Zebrafish as an Emerging Model System in the Global South: Two Decades of Research in Brazil

et al. 2020

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The zebrafish (Danio rerio) is an emerging model system in several research areas worldwide, especially in the Global South. In this context, the present study revised the historical use and trends of zebrafish as experimental models in Brazil. The data concerning the bibliometric parameters, research areas, geographic distribution, experimental design, zebrafish strain, and reporter lines, as well as recent advances were revised. In addition, the comparative trends of Brazilian and global research were discussed. Revised data showed the rapid growth of Brazilian scientific production using zebrafish as a model, especially in three main research areas (Neuroscience &and Behavior, Pharmacology and Toxicology, and Environment/Ecology). Studies were conducted in 19 Brazilian states (70.37%), confirming the wide geographic distribution and importance of zebrafish research. Results indicated that research related to toxicological approaches are widespread in Global South countries such as Brazil. Studies were performed mainly using in vivo tests (89.58%) with adult fish (59.75%) and embryos (30.67%). Moreover, significant research gaps and recommendations for future research are presented. The present study shows that the zebrafish is a suitable vertebrate model system in the Global South.

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“…Sperm with positive rhodamine staining (green fluorescence) was considered to have functional mitochondria. In contrast, non-functional mitochondria were characterized by negative rhodamine staining (sperm without fluorescence) (He and Woods 2004;Bautista et al 2018). The proportion of sperm that emits green fluorescence compared with total sperm (green or no fluorescence) determined the rate of mitochondrial function.…”

Section: Functional Mitochondriamentioning

confidence: 99%

Short Communication: Effect of cryopreservation on ultrastructure and mitochondrial function of albino Pangasius catfish spermatozoa

et al. 2020

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Abstract. Hasanah U, Binawanto, Alimuddin A, Boediono A, Kursini E. 2020. Short Communication: Effect of cryopreservation on ultrastructure and mitochondrial function of albino Pangasius catfish spermatozoa. Biodiversitas 21: 4524-4528. Cryopreservation techniques have been carried out on many endangered species and animals with unique characteristics. Successful cryopreservation techniques vary between species depending on various factors. The study used cryopreserved spermatozoa of the albino Pangasius catfish as samples. The cryopreserved spermatozoa were analyzed by its ultrastructure, functional mitochondria, and viability. The cryopreservation was performed using a combination of 10% methanol, skim milk, and fish ringer extender. A deep freezer is used for cryopreservation at -80 °C with a storage period of 14 days. Observations were made on fresh spermatozoa, post-equilibration spermatozoa, and frozen-thaw spermatozoa. This study found there were differences in ultrastructure and morphology in the three treatments. Fresh spermatozoa and post-equilibration spermatozoa appeared intact membrane, mitochondrial, and flagellar structures. In contrast, in frozen-thaw spermatozoa, there was damage to the cell membrane. The study showed different percentages of yields on functional mitochondria of fresh spermatozoa (98 ± 2%), spermatozoa post-equilibration (57 ± 7%), frozen-thaw spermatozoa (42 ± 3.21%). Cell viability showed that there were differences in viability of fresh spermatozoa and frozen-thaw (p <0.05), the results of fresh spermatozoa (92 ± 0.57%) spermatozoa post-equilibration (80 ± 3.51%), frozen-thaw spermatozoa (61 ± 2.30%).The study concluded that the spermatozoa cryopreservation affects the ultrastructure, mitochondrial function, and viability in albino Pangasius catfish spermatozoa.

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The herbicide atrazine affects sperm quality and the expression of antioxidant and spermatogenesis genes in zebrafish testes

Cited by 29 publications

References 42 publications

Alleviating effects of β‐glucan inOreochromis niloticuson growth performance, immune reactions, antioxidant, transcriptomics disorders and resistance toAeromonas sobriacaused by atrazine

Alleviating effects of β‐glucan inOreochromis niloticuson growth performance, immune reactions, antioxidant, transcriptomics disorders and resistance toAeromonas sobriacaused by atrazine

Zebrafish as an Emerging Model System in the Global South: Two Decades of Research in Brazil

Short Communication: Effect of cryopreservation on ultrastructure and mitochondrial function of albino Pangasius catfish spermatozoa

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