The helminth parasite proteome at the host–parasite interface – Informing diagnosis and control

Ree, Anna M. van der; Mutapi, Francisca

doi:10.1016/j.exppara.2015.06.007

Cited by 17 publications

(10 citation statements)

References 126 publications

(171 reference statements)

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“…Other molecules are secreted from the gut, excretory pores and surface cuticle/tegument into the local microenvironment, often the host intestine [2][3][4]. For many years the paucity of material made these secretions extremely difficult to analyse but the application of mass spectrometry-based proteomics techniques has allowed a detailed understanding of the type and abundance of the various proteins present in soluble helminth secretions (reviewed in [5]). However, until quite recently the contribution of extracellular vesicles (EVs; see glossary) to helminth secretomes has been overlooked.…”

Section: Molecular Communication At the Host-parasite Interfacementioning

confidence: 99%

Extracellular Vesicle Biogenesis in Helminths: More than One Route to the Surface?

Torre-Escudero

Bennett

Clarke

et al. 2016

Trends in Parasitology

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13The quite recent discovery that parasites release extracellular vesicles (EVs) that can transfer 14 a range of effector molecules to host cells has made us re-think our understanding of the host-15 parasite interface. In this opinion article we will consider how recent proteomics and 16 transcriptomics studies, together with ultrastructural observations, suggest that more than one 17 mechanism of EV biogenesis can occur in helminths. We propose that distinct EV sub-types 18 have roles in immune-modulation and repair of drug-induced damage, and put forward the 19 case for targeting EV biogenesis pathways to achieve parasite control. In doing so we raise a 20 number of outstanding research questions that must be addressed before this can happen. released by mammalian cells (Table 1) nematodes has yet to be documented although exosomes released by C. elegans epithelial The mechanism of bleb formation in helminths has yet to be determined, but it is 214 thought to be a calcium-dependent process [63,64]. MVs also bud directly from the plasma 215 membrane in response to raised intracellular Ca 2+ levels [65]. Given these biochemical and 216 structural similarities, it is conceivable that parasite blebs are formed using the same Concluding remarks 224The discovery of parasite-derived EVs has changed our view of the host-parasite interface. 225The formation of EVs enables the parasite to release a range of effector molecules (including

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Section: Molecular Communication At the Host-parasite Interfacementioning

confidence: 99%

Extracellular Vesicle Biogenesis in Helminths: More than One Route to the Surface?

Torre-Escudero

Bennett

Clarke

et al. 2016

Trends in Parasitology

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“…Better understanding of a parasite’s adaptations to its way of life and the complexity of interactions between the parasite and the host is possible by introduction of modern techniques of proteomic and genomic research [ 10 – 13 ]. Proteomic studies of helminths have shown new aspects in the parasite-host interrelations involving selected species of tapeworms Echinococcus granulosus [ 14 – 19 ] , Echinococcus multilocularis [ 20 , 21 ] and Taenia solium [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of immunogenic proteins of the cysticercoid of Hymenolepis diminuta

et al. 2017

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BackgroundA wide range of molecules are used by tapeworm metacestodes to establish successful infection in the hostile environment of the host. Reports indicating the proteins in the cestode-host interactions are limited predominantly to taeniids, with no previous data available for non-taeniid species. A non-taeniid, Hymenolepis diminuta, represents one of the most important model species in cestode biology and exhibits an exceptional developmental plasticity in its life-cycle, which involves two phylogenetically distant hosts, arthropod and vertebrate.ResultsWe identified H. diminuta cysticercoid proteins that were recognized by sera of H. diminuta-infected rats using two-dimensional gel electrophoresis (2DE), 2D-immunoblotting, and LC-MS/MS mass spectrometry. Proteomic analysis of 42 antigenic spots revealed 70 proteins. The largest number belonged to structural proteins and to the heat-shock protein (HSP) family. These results show a number of the antigenic proteins of the cysticercoid stage, which were present already in the insect host prior to contact with the mammal host. These are the first parasite antigens that the mammal host encounters after the infection, therefore they may represent some of the molecules important in host-parasite interactions at the early stage of infection.ConclusionsThese results could help in understanding how H. diminuta and other cestodes adapt to their diverse and complex parasitic life-cycles and show universal molecules used among diverse groups of cestodes to escape the host response to infection.Electronic supplementary materialThe online version of this article (10.1186/s13071-017-2519-4) contains supplementary material, which is available to authorized users.

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“…However, for potential future clinical usage of these medicines, a thorough examination of their mode of action is imperative. This was further confirmed in a recent review on helminth parasites where no mode of action studies were recorded but recognised as important for further study (van der Ree and Mutapi, 2015). It appears that mode of action studies are complex when studing the different parasites as each parasite has a complex life cycle and the mode of action would need to be studied at every stage within that life cycle in order to get a bigger picture.…”

Section: Mode Of Action Studiesmentioning

confidence: 84%

A review of the traditional use of southern African medicinal plants for the treatment of selected parasite infections affecting humans

Cock

Selesho

Vuuren

2018

Journal of Ethnopharmacology

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The helminth parasite proteome at the host–parasite interface – Informing diagnosis and control

Cited by 17 publications

References 126 publications

Extracellular Vesicle Biogenesis in Helminths: More than One Route to the Surface?

Extracellular Vesicle Biogenesis in Helminths: More than One Route to the Surface?

Identification of immunogenic proteins of the cysticercoid of Hymenolepis diminuta

A review of the traditional use of southern African medicinal plants for the treatment of selected parasite infections affecting humans

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