The heat shrink tube technology – a simple method for making hair cross sections

Wagner, Gunnar; Heine, M.; Sachse, Michael Max

doi:10.1111/ddg.12410

Cited by 4 publications

(7 citation statements)

References 4 publications

(4 reference statements)

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“…We also experienced this in our previous trials with hair embedding in paraffin, making impossible to ascertain the percentage of hairs affected. Heat shrink tube (HST) technology has been proposed as a method to overcome this problem, but also requires specialized skills and equipment 4 …”

Section: Discussionmentioning

confidence: 99%

“…2 Scanning electron microscopy (SEM) is the current gold standard for detecting this abnormality, but access to this equipment is limited and usually expensive. 2,5 More recently, hair cross-sectioning has been proposed as an alternative 1,3,4 because of typical kidney-like or triangular appearance of hair in cross-section analysis. 1,3,4 However, the diagnosis may only be ascertained if the structural anomaly is detected in more than 50% of hairs.…”

Section: F I G U R Ementioning

confidence: 99%

“…2,5 More recently, hair cross-sectioning has been proposed as an alternative 1,3,4 because of typical kidney-like or triangular appearance of hair in cross-section analysis. 1,3,4 However, the diagnosis may only be ascertained if the structural anomaly is detected in more than 50% of hairs. 5 A typical hair mount would not suffice because it is crucial to visualize the hair in cross-section.…”

Section: F I G U R Ementioning

confidence: 99%

“…Heat shrink tube (HST) technology has been proposed as a method to overcome this problem, but also requires specialized skills and equipment. 4 Using our technique, we optimize the number of hair crosssections that are visualized from each hair bundle, allowing for an estimation of the percentage of pathologically changed hair shafts.…”

Section: F I G U R Ementioning

confidence: 99%

“…To overcome the flaws of other hair cross‐section methods previously described, 1,3,4 we used an epoxy embedding medium to maintain the hair strands intact and close together through the whole processing.…”

Section: Case Reportmentioning

confidence: 99%

See 4 more Smart Citations

Hair cross‐sectioning in uncombable hair syndrome: An epoxy embedding technique

et al. 2020

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Uncombable hair syndrome is a rare disorder of the hair shaft that leads to silvery and unruly hair. The hair shaft anomaly is characterized by a longitudinal groove that is detected by scanning electronic microscopy-considered to be the gold standard for diagnosis. Recently, hair cross-sectioning has been reported as a viable alternative, but currently available methods still have some flaws, especially because of hair samples' processing specificities. Here, we present two cases of uncombable hair syndrome and a new embedding technique using epoxy to perform the diagnosis.

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Section: Discussionmentioning

confidence: 99%

Section: F I G U R Ementioning

confidence: 99%

Section: F I G U R Ementioning

confidence: 99%

Section: F I G U R Ementioning

confidence: 99%

Section: Case Reportmentioning

confidence: 99%

See 3 more Smart Citations

Hair cross‐sectioning in uncombable hair syndrome: An epoxy embedding technique

et al. 2020

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High-throughput phenotyping methods for quantifying hair fiber morphology

Lasisi

Zaidi

Webster

et al. 2021

Sci Rep

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Quantifying the continuous variation in human scalp hair morphology is of interest to anthropologists, geneticists, dermatologists and forensic scientists, but existing methods for studying hair form are time-consuming and not widely used. Here, we present a high-throughput sample preparation protocol for the imaging of both longitudinal (curvature) and cross-sectional scalp hair morphology. Additionally, we describe and validate a new Python package designed to process longitudinal and cross-sectional hair images, segment them, and provide measurements of interest. Lastly, we apply our methods to an admixed African-European sample (n = 140), demonstrating the benefit of quantifying hair morphology over classification, and providing evidence that the relationship between cross-sectional morphology and curvature may be an artefact of population stratification rather than a causal link.

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High-throughput phenotyping methods for quantifying hair fiber morphology

Lasisi

Zaidi

Webster

et al. 2020

Preprint

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Quantifying the continuous variation in human scalp hair morphology is of interest to anthropologists, geneticists, dermatologists and forensic scientists, but existing methods for studying hair form are time-consuming and not widely used. Here, we present a high-throughput sample preparation protocol for the imaging of both longitudinal (curvature) and cross-sectional scalp hair morphology. Additionally, we describe and validate a new Python package designed to process longitudinal and cross-sectional hair images, segment them, and provide measurements of interest. Lastly, we apply our methods to an admixed African-European sample (n=140), demonstrating the benefit of quantifying hair morphology over qualitative classification or racial categories, and providing evidence against the long-held belief that cross-sectional morphology predicts curvature.

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The heat shrink tube technology – a simple method for making hair cross sections

Cited by 4 publications

References 4 publications

Hair cross‐sectioning in uncombable hair syndrome: An epoxy embedding technique

Hair cross‐sectioning in uncombable hair syndrome: An epoxy embedding technique

High-throughput phenotyping methods for quantifying hair fiber morphology

High-throughput phenotyping methods for quantifying hair fiber morphology

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