The health-promoting anthocyanin petanin in <i>Lycium ruthenicum</i> fruit: a promising natural colorant

Zeng, Shaohua; Lin, Shuang; Wang, Zhiqiang; Zong, Yuan; Wang, Ying

doi:10.1080/10408398.2023.2225192

Cited by 4 publications

(4 citation statements)

References 109 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Anthocyanins, naturally occurring avonoid pigments found in a variety of fruits and vegetables, have been extensively investigated for their potential biological properties (Chen et al 2022). Lycium ruthenicum Murray is an optimal botanical resource for anthocyanins extraction due to its high content of these compounds (Zeng et al 2023). Our previous studies have provided evidence that ALR effectively hinders the growth and spread of HepG2 cells, while also promoting cell death and arresting the G2/M phase cycle (Fan et al 2022).…”

Section: Discussionmentioning

confidence: 99%

“…Its fruits, which have a dark violet-black appearance, have been widely used in China for over 2000 years as health food and traditional Chinese medicine (Wang et al 2018a). Multiple studies have provided evidence supporting the various biological properties of black goji, including its anti-in ammatory, anti-oxidant, and anti-tumor properties, among which the main bioactive ingredient is anthocyanins (Zeng et al 2023;Han et al 2022). Anthocyanins are a type of avonoid compounds that can be found in various plants and play a pivotal role in the prevention and control of cancer (Chen et al 2022).…”

Section: Introductionmentioning

confidence: 99%

“…Anthocyanins are a type of avonoid compounds that can be found in various plants and play a pivotal role in the prevention and control of cancer (Chen et al 2022). For example, the extraction of anthocyanins from blackberries, mulberries, grapes, and black soybeans have demonstrated promising potential in terms of their anticancer activity (Zeng et al 2023). Mechanistically speaking, anthocyanins are primarily attributed to their ability to induce apoptosis and autophagy processes within cells (Long et al 2018).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Anthocyanins from Lycium ruthenicum Murray inhibit HCC tumor angiogenesis and promote autophagic apoptosis through AMPK/PI3K/AKT/mTOR pathway

Wei,

Cheng,

Fan

et al. 2023

Preprint

View full text Add to dashboard Cite

Purpose Angiogenesis is a crucial factor in the initiation, progression and metastasis of tumor. The exploration for novel anti-angiogenic agents from natural sources has garnered significant attention. Our previous study confirmed the inhibitory effects of anthocyanins obtained from Lycium ruthenicum Murray (ALR) on the growth and metastasis of HepG2 cells, yet its potential to suppress neovascularization in hepatocellular carcinoma (HCC) remains unknown. This research aimed to assess the anti-angiogenic effect of ALR in HCC and elucidate its underlying mechanism. Methods Cell viability, colony formation, migration, invasion, and tubule formation were performed to evaluate the effect of ALR on EA.hy926 cells. Transcriptomics sequencing was performed to analyze the functional enrichment and pathway prediction of differentially expressed genes (DEGs). The formation of autophagosomes and autophagy-related proteins level were observed by AO staining, MDC staining, GFP-LC3-Adenovirus infection and western blot analysis. The relationship between autophagy and cell proliferation, angiogenesis, as well as AMPK/PI3K/AKT/mTOR pathway were analyzed in EA.hy926 cells treated with ALR in the presence or absence of 3-Methyladenine (3-MA, autophagy inhibitor). The transplanted hepatocellular carcinoma (HCC) model was established using HepG2 cells, and the therapeutic effect and underlying mechanism of ALR on HCC were assessed by H&E staining, TUNEL assay, immunofluorescence and immunohistochemistry analysis. Results Our findings demonstrated that ALR significantly suppressed the viability, proliferation, migration, invasion and angiogenesis of EA.hy926 cells in a dose-dependent pattern. The transcriptomic sequencing results revealed a close association between ALR inhibition of angiogenesis and autophagy, primarily enriched in the PI3K/AKT, AMPK and mTOR signaling pathways. In vitro outcomes further validated that ALR significantly augmented the presence of autophagosomes, enhanced the levels of Beclin-1, LC3-II/LC3-I, and p-AMPK while concurrently suppressing the levels of p62, p-PI3K, p-AKT, and p-mTOR. Notably, these effects were effectively counteracted using 3-Methyladenine. In vivo xenograft results demonstrated that ALR significantly inhibited the growth of HCC tumors. Mechanistically, ALR activated autophagy to suppress the expression of CD31, VEGF, and VEGFR2 in tumor tissues. Additionally, ALR induced autophagy-dependent apoptosis by up-regulating the levels of Bax, Cleaved caspase-3, and Cleaved caspase-9 while down-regulating the Bcl-2 level. Furthermore, it was discovered that ALR regulates autophagy by activating AMPK and suppressing the activity of PI3K/AKT/mTOR axis. Conclusion In combination, our data suggest that ALR executes its anti-HCC activity by inhibiting tumor angiogenesis and promoting autophagic apoptosis through regulation of the AMPK/PI3K/AKT/mTOR signaling cascade. The utilization of ALR as a natural adjuvant against tumors may hold immense potential in the foreseeable future.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Anthocyanins from Lycium ruthenicum Murray inhibit HCC tumor angiogenesis and promote autophagic apoptosis through AMPK/PI3K/AKT/mTOR pathway

Wei,

Cheng,

Fan

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Anthocyanins are flavonoid compounds that are commonly present in various parts of plants, such as the flowers, fruits, leaves, roots, and stems, adding to their vivid hue [ 1 ]. These vibrant colors are crucial for attracting pollinators, facilitating seed dispersion, and providing protection against damage from ultraviolet radiation [ 2 , 3 , 4 ]. Anthocyanin pigments have been the subject of extensive research, leading to notable scientific advancements over the last 150 years [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

The R2R3-MYB Transcriptional Repressor TgMYB4 Negatively Regulates Anthocyanin Biosynthesis in Tulips (Tulipa gesneriana L.)

Hu,

Liang,

Sun

et al. 2024

IJMS

View full text Add to dashboard Cite

Anthocyanins play a paramount role in color variation and significantly contribute to the economic value of ornamental plants. The conserved activation complex MYB-bHLH-WD40 (MBW; MYB: v-myb avian myeloblastosis viral oncogene homolog; bHLH: basic helix–loop–helix protein; WD40:WD-repeat protein) involved in anthocyanin biosynthesis has been thoroughly researched, but there have been limited investigations into the function of repressor factors. In this study, we characterized TgMYB4, an R2R3-MYB transcriptional repressor which is highly expressed during petal coloration in red petal cultivars. TgMYB4-overexpressing tobaccos exhibited white or light pink petals with less anthocyanin accumulation compared to control plants. TgMYB4 was found to inhibit the transcription of ANTHOCYANIDIN SYNTHASE (TfANS1) and DIHYDRO-FLAVONOL-4-REDUCTASE (AtDFR), although it did not bind to their promoters. Moreover, the TgMYB4 protein was able to compete with the MYB activator to bind to the :bHLHprotein, thereby suppressing the function of the activator MBW complex. These findings demonstrate that TgMYB4 plays a suppressive role in the regulation of anthocyanin synthesis during flower pigmentation.

show abstract

Gellan Gum and Polyvinyl Alcohol Based Triple-Layer Films Enriched with Alhagi sparsifolia Flower Extract: Preparation, Characterization, and Application of Dried Shrimp Preservation

Yue,

Cheng,

Liu

et al. 2023

Foods

View full text Add to dashboard Cite

To meet the demand for biobased packaging and minimize the oxidation of dried aquatic goods during storage, we created a triple-layer film (TF) with antioxidant capacity. The film was produced using polyvinyl alcohol (PVA) as the protective layer, gellan gum (GG)/PVA composite incorporating Alhagi sparsifolia flower extract (AFE) as the anti-oxidative capability layer, and GG as the anti-oxidative capacity slow-release control layer. The TFs with different AFE additions were characterized and compared to a single-layer film (SF) made of the same material. The results demonstrate that adding AFE to films degraded their water vapour and oxygen barrier properties as well as their tensile strength, but increased their light barrier properties, elongation at break, and anti-oxidative capability. The three-layer structure increased the light, water vapour, and oxygen barrier qualities of films, as well as their slow-release anti-oxidative capability. The application experiment revealed that the inclusion of AFE might aid in the preservation of dried prawn quality. Using TF supplemented with 5 (w/v) AFE to package the dried shrimps reduced the TBARS value by 47.5%. Our research indicated that TFs containing AFE have a wide range of possible applications in dried shrimp preservation.

show abstract

The health-promoting anthocyanin petanin in Lycium ruthenicum fruit: a promising natural colorant

Cited by 4 publications

References 109 publications

Anthocyanins from Lycium ruthenicum Murray inhibit HCC tumor angiogenesis and promote autophagic apoptosis through AMPK/PI3K/AKT/mTOR pathway

Anthocyanins from Lycium ruthenicum Murray inhibit HCC tumor angiogenesis and promote autophagic apoptosis through AMPK/PI3K/AKT/mTOR pathway

The R2R3-MYB Transcriptional Repressor TgMYB4 Negatively Regulates Anthocyanin Biosynthesis in Tulips (Tulipa gesneriana L.)

Gellan Gum and Polyvinyl Alcohol Based Triple-Layer Films Enriched with Alhagi sparsifolia Flower Extract: Preparation, Characterization, and Application of Dried Shrimp Preservation

Contact Info

Product

Resources

About