The gut and feed residue microbiota changing during the rearing of Hermetia illucens larvae

Cifuentes, Yina; Glaeser, Stefanie P.; Mvie, Jacques B.; Bartz, Jens-Ole; Müller, Ariane; Gutzeit, Herwig O.; Vilcinskas, Andreas; Kämpfer, Peter

doi:10.1007/s10482-020-01443-0

Cited by 41 publications

(79 citation statements)

References 62 publications

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“…Previous studies have reported inconsistent results on the significance of altered substrate bacterial community during BSFL rearing (Bruno et al, 2019;Jiang et al, 2019;Cifuentes et al, 2020). Our study confirmed that BSFL rearing dramatically changes the substrate bacterial community and physicochemical properties and composition.…”

Section: Common Fly Associated Bacteria Dominate the Rearing Residuesupporting

confidence: 87%

“…Consistent with previous research by Jiang et al (2019) and Wynants et al (2018), this study observed a reduction in bacterial richness (Table 2) in the residue in comparison to the substrate over the rearing duration. In contrast to Wynants et al (2018) and Jiang et al (2019), Bruno et al (2019) did not observe significant differences in the bacterial community between substrates and residues, and Cifuentes et al (2020) observed an increase in bacterial richness. Different abiotic (e.g., temperature, substrate nutrient content, and pH) and biotic (e.g., initial substrate bacterial communities) factors known to influence microbial ecology and the presence and stability of antimicrobial proteins by BSFL (De Smet et al, 2018;Vogel et al, 2018) may provide some explanation for discrepancies in findings among studies.…”

Section: Common Fly Associated Bacteria Dominate the Rearing Residuecontrasting

confidence: 80%

“…Our study and that of Jiang et al (2019) had a 2-3-fold higher larval density and feed was provided less frequently when compared to Bruno et al (2019), thus allowing more time for larval digestion ( Table 3). Cifuentes et al (2020) provided an insufficient rearing protocol to allow comparison with the other studies. A further study focusing more on microbial dynamics in BSFL under different rearing parameters is therefore recommended.…”

Section: Common Fly Associated Bacteria Dominate the Rearing Residuementioning

confidence: 99%

“…Genera in the residues at the time of larval harvest are ubiquitous in the environment (e.g., soil, water, digestive tracts of humans, and farmed animals) and have been previously identified in different life stages of the BSF (Table 3; Bruno et al, 2019;Cifuentes et al, 2020;Klammsteiner et al, 2020;Raimondi et al, 2020;Zhan et al, 2020), Musca domestica (Zurek et al, 2000;Su et al, 2010;Gupta et al, 2012), and Lucilia sericata (Singh et al, 2014). Bacteria commonly associated with BSFL residues (Table 3) are from the Lactobacillaceae, Bacillaceae, Enterobacteriaceae, Planococcaceae, and Flavobacteriaceae families.…”

Section: Common Fly Associated Bacteria Dominate the Rearing Residuementioning

confidence: 99%

“…The results of this study support this hypothesis. Recurring taxa in the residues belong to the phyla Firmicutes, Proteobacteria, and Bacteroidetes, which are the main intestinal bacteria in fly larvae (Zurek et al, 2000;Jeon et al, 2011;Boccazzi et al, 2017;Scully et al, 2017;Cifuentes et al, 2020;Liu et al, 2020). Providencia spp., Dysgonomonas spp., Morganella spp., and Proteus spp.…”

Section: Common Fly Associated Bacteria Dominate the Rearing Residuementioning

confidence: 99%

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Identification of Bacteria in Two Food Waste Black Soldier Fly Larvae Rearing Residues

et al. 2020

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Significant economic, environmental, and social impacts are associated with the avoidable disposal of foods worldwide. Mass-rearing of black soldier fly (Hermetia illucens) larvae using organic wastes and food- and agro-industry side products is promising for recycling resources within the food system. One current challenge of this approach is ensuring a reliable and high conversion performance of larvae with inherently variable substrates. Research has been devoted to increasing rearing performance by optimizing substrate nutrient contents and ratios, while the potential of the substrate and larval gut microbiota to increase rearing performance remains untapped. Since previous research has focused on gut microbiota, here, we describe bacterial dynamics in the residue (i.e., the mixture of frass and substrate) of black soldier fly larvae reared on two food wastes (i.e., canteen and household waste). To identify members of the substrate and residue microbiota, potentially associated with rearing performance, bacterial dynamics were also studied in the canteen waste without larvae, and after inactivation by irradiation of the initial microbiota in canteen waste. The food waste substrates had similar microbiota; both were dominated by common lactic acid bacteria. Inactivation of the canteen waste microbiota, which was dominated by Leuconostoc, Bacillus, and Staphylococcus, decreased the levels of all rearing performance indicators by 31–46% relative to canteen waste with the native microbiota. In both food waste substrates, larval rearing decreased the bacterial richness and changed the physicochemical residue properties and composition over the rearing period of 12 days, and typical members of the larval intestinal microbiota (i.e., Providencia, Dysgonomonas, Morganella, and Proteus) became more abundant, suggesting their transfer into the residue through excretions. Future studies should isolate members of these taxa and elucidate their true potential to influence black soldier fly mass-rearing performance.

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Section: Common Fly Associated Bacteria Dominate the Rearing Residuesupporting

confidence: 87%

Section: Common Fly Associated Bacteria Dominate the Rearing Residuecontrasting

confidence: 80%

Section: Common Fly Associated Bacteria Dominate the Rearing Residuementioning

confidence: 99%

Section: Common Fly Associated Bacteria Dominate the Rearing Residuementioning

confidence: 99%

Section: Common Fly Associated Bacteria Dominate the Rearing Residuementioning

confidence: 99%

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Identification of Bacteria in Two Food Waste Black Soldier Fly Larvae Rearing Residues

et al. 2020

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A Preliminary Comparison on Faecal Microbiomes of Free-Ranging Large Baleen (Balaenoptera musculus, B. physalus, B. borealis) and Toothed (Physeter macrocephalus) Whales

et al. 2021

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Large baleen and toothed whales play crucial ecological roles in oceans; nonetheless, very little is known about their intestinal microbiomes. Based on striking differences in natural history and thus in feeding behaviours, it can be expected that intestinal microbiomes of large baleen whales and toothed whales are different. To test this hypothesis, the phylogenetic composition of faecal microbiomes was investigated by a 16S rRNA gene amplicon sequence–based approach for Bacteria and Archaea. Faecal samples from free-ranging large whales collected off the Azores Archipelago (Portugal) were used, comprising 13 individual baleen whales (one sei, two blue and ten fin whales) and four sperm whales. The phylogenetic composition of the Bacteria faecal microbiomes of baleen and toothed whales showed no significant differences at the phylum level. However, significant differences were detected at the family and genus levels. Most abundant phyla were Firmicutes, Bacteroidetes, Proteobacteria, Tenericutes and Spirochaeta. Few highly abundant bacterial genera were identified as key taxa with a high contribution to differences among baleen and toothed whales microbiomes. Only few archaeal sequences were detected, primarily Methanomassiliicoccales representing potential methanogenic Archaea. This is the first study that directly compares the faecal bacterial and archaeal microbiomes of free-ranging baleen and toothed whales which represent the two parvorders of Cetacea which members are fully aquatic large mammals which were evolutionary split millions of years ago.

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Changes in Bacterial Community Structure Across the Different Life Stages of Black Soldier Fly (Hermetia illucens)

et al. 2022

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The gut and feed residue microbiota changing during the rearing of Hermetia illucens larvae

Cited by 41 publications

References 62 publications

Identification of Bacteria in Two Food Waste Black Soldier Fly Larvae Rearing Residues

Identification of Bacteria in Two Food Waste Black Soldier Fly Larvae Rearing Residues

A Preliminary Comparison on Faecal Microbiomes of Free-Ranging Large Baleen (Balaenoptera musculus, B. physalus, B. borealis) and Toothed (Physeter macrocephalus) Whales

Changes in Bacterial Community Structure Across the Different Life Stages of Black Soldier Fly (Hermetia illucens)

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