Identification of Bacteria in Two Food Waste Black Soldier Fly Larvae Rearing Residues

Gold, Moritz; Allmen, Fabienne von; Zurbrügg, Christian; Zhang, Jibin; Mathys, Alexander

doi:10.3389/fmicb.2020.582867

Cited by 49 publications

(62 citation statements)

References 95 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, Dysgonomonas is fastidious and grows very slowly, mainly as satellite bacteria, supporting that this dominant genus could be passed unnoticed unless a molecular approach to determine the microbial community composition is performed ( Carda-Diéguez et al, 2014 ). Moreover, Dysgonomonas has been found to be dominant in crustaceans such as P. clarkii and Eriocheir sinensis as well as fly larvae ( Feng et al, 2019 ; Gold et al, 2020 ). For E. sinensis , the relative abundance of Dysgonomonas was the highest in juveniles and relatively low in larvae and adults ( Wang et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Intestinal Microbiota During the Different Growth Stages of Red Swamp Crayfish (Procambarus clarkii)

Xie

Zhang²,

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

This study aimed to determine the effect of the growth stage of Procambarus clarkii on their intestinal microbiota. Intestinal samples of five different growth stages of P. clarkii (first instar, second instar, third instar, juvenile, and adult) from laboratory culture were analyzed through the Illumina MiSeq high-throughput sequencing platform to determine the intestinal microbiome of crayfish. The alpha diversity decreased along with the growth of the crayfish, with the relative abundance of the microbiota changing among stages; crayfish at closer development stages had a more comparable intestinal microbiota composition. A comparative analysis by principal component analysis and principal coordinate analysis showed that there were significant differences in the intestinal microbiota of crayfish among the different growth stages, except for the first two stages of larval crayfish, and the intestinal microbiota showed a consistent progression pattern from the larval stage to the juvenile stage. Some microbiota showed stage specificity, which might be the characteristic microbiota of different stages of growth. According to FAPROTAX functional clustering analysis, the three stages of larvae were clustered together, while the juvenile and adult stages were clustered separately according to the growth stage, indicating that, in the early stages of larval development, the function of the intestinal flora was similar; as the body grew and developed, the composition and function of the intestinal microbiota also changed.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparison of the Intestinal Microbiota During the Different Growth Stages of Red Swamp Crayfish (Procambarus clarkii)

Xie

Zhang²,

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Considering that organic residue may already contain high microbial loads, that larvae produce their excreta in the environment in which they grow and that they are processed as whole larvae with intact intestinal tracts, the risks of encountering microbiological pathogens in the final product are high (Bruno et al, 2019;De Smet et al, 2018;Gold et al, 2020;Lalander et al, 2019;Shelomi et al, 2020;Tanga et al, 2021;Wynants et al, 2019). Food waste should be stabilised to reduce these risks upstream of production.…”

Section: Introductionmentioning

confidence: 99%

Comparison of black soldier fly larvae pre-treatments and drying techniques on the microbial load and physico-chemical characteristics

Saucier

M’ballou

Ratti

et al. 2022

JIFF

View full text Add to dashboard Cite

Black soldier fly larvae (BSFL) are good candidates for upcycling wet organic residuals. Like other unprocessed raw animal products, BSFL require processing to prevent spoilage and degradation during storage and to facilitate their use as feed ingredients. In this study, hot-air drying and freeze-drying were examined as means to ensure long-term preservation. Pre-treatments of larvae, such as puncturing, blanching (40 s) and scalding (2, 4, 6 and 8 min) in boiling water reduced drying times, most likely by affecting the integrity of the wax-coated cuticle that protects the larvae against desiccation. Overall, the larvae dried two to six times faster using hot-air compared to freeze-drying, and larvae pre-treatments were proven to effectively improve drying efficacy. Pre-treating larvae in boiling water followed by a shorter drying time with hot air was effective at reducing primary and secondary oxidation as well as darkening/browning (colour lightness, L* value) compared to the untreated control (raw-thawed) larvae. The larvae pre-treatments in boiling water also led to a significant reduction in microbial load (3.21 to 4.83 log) in the dry product compared to the control. BSFL powder, produced from grinding thawed larvae that were pre-treated for 4 min in boiling water before being dried in hot air (60 °C; 6 h), had a water activity below 0.4. This led to a relatively stable product with limited colour changes over a 30-day storage period. These processing treatments also resulted in a product with no detectable Salmonella and Escherichia coli counts ranging from 100 to 1000 cfu/g. Overall, the powdered BSFL product was deemed suitable to incorporate into pelleted feed under the current regulations in Canada.

show abstract

“…The process performance of biowastes with BSFL was estimated using values provided in the literature of typical BSFL performance metrics, for example, larval mass, bioconversion rate, and waste reduction (Banks et al, 2014;Bava et al, 2019;Diener et al, 2009;Gold et al, 2021;Gold et al, 2020a;Jucker et al, 2017; ., 2019;Liu et al, 2018;Meneguz et al, 2018;Miranda et al, 2019;Nguyen et al, 2013;Nyakeri et al, 2019;Rehman et al, 2017;Somroo et al, 2019;Spranghers et al, 2017;Tschirner & Simon, 2015). The feeding rate and treatment time within these studies were 40-250 mg waste/larva/ day and 6-27 days, respectively.…”

Section: Review Of Performance Metricsmentioning

confidence: 99%

“…Samples were pit latrine sludge (n = 3), human faeces with sawdust (n = 4), fruit and vegetable waste (n = 1), vegetable waste (n = 2), poultry feed (n = 1), food waste from hotels (n = 9), food waste from restaurants (n = 2), cereal-based by-products (n = 1), fruit waste (n = 4), and slaughterhouse waste (n = 2). To generalise our approach, values provided in the literature for the same parameters were summarised (Giromini et al, 2017;Gold et al 2020a;Gold et al, 2020b;Gold et al, 2021;Liu et al, 2018;Meneguz et al, 2018;Shumo et al, 2019;Vruggink, 2020).…”

Section: Biowaste Analyses For Mixture Formulationmentioning

confidence: 99%

Efficient and safe substrates for black soldier fly biowaste treatment along circular economy principles

Gold

Ireri²,

Zurbrügg

et al. 2021

Detritus

Self Cite

View full text Add to dashboard Cite

Black soldier fly larvae (BSFL) treatment is an emerging technology for the valorisation of nutrients from biowaste. Selecting suitable substrates for BSFL treatment is a frequent challenge for researchers and practitioners. We conducted a systematic assessment of BSFL treatment substrates in Nairobi, Kenya to source more substrate for upscaling an existing BSFL treatment facility. The applied approach is universal and considers four criteria: 1) substrate availability and costs, 2) BSFL process performance, 3) product safety, and 4) waste recovery hierarchy. Data were collected from previous waste assessments or semi-structured key informant interviews and sight tours of waste producers. Waste nutritional composition and BSFL process performance metrics were summarised in the “BSFL Substrate Explorer”, an open-access web application that should facilitate the replication of such assessments. We show that most biowaste in Nairobi is currently not available for facility upscaling due to contamination with inorganics and a lack of affordable waste collection services. A mixture of human faeces, animal manure, fruit/vegetable waste, and food waste (with inorganics) should be pursued for upscaling. These wastes tend to have a lower treatment performance, but in contrast to cereal-based byproducts, food industry byproducts, and segregated food waste, there is no conflict with animal feed utilization. The traceability of substrates, source control, and post-harvest processing of larvae are required to ensure feed safety. The criteria presented here ensures the design of BSFL treatment facilities based on realistic performance estimates, the production of safe insect-based products, and environmental benefits of products compared to the status quo.

show abstract

Identification of Bacteria in Two Food Waste Black Soldier Fly Larvae Rearing Residues

Cited by 49 publications

References 95 publications

Comparison of the Intestinal Microbiota During the Different Growth Stages of Red Swamp Crayfish (Procambarus clarkii)

Comparison of the Intestinal Microbiota During the Different Growth Stages of Red Swamp Crayfish (Procambarus clarkii)

Comparison of black soldier fly larvae pre-treatments and drying techniques on the microbial load and physico-chemical characteristics

Efficient and safe substrates for black soldier fly biowaste treatment along circular economy principles

Contact Info

Product

Resources

About