The growth, physiology and toxigenic potential of Bacillus cereus in cooked rice during storage temperature abuse

Cronin, Ultan P.; Wilkinson, Martin G.

doi:10.1016/j.foodcont.2008.10.018

Cited by 14 publications

(7 citation statements)

References 31 publications

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“…. This value is slightly higher than that reported by [15] in which the total aerobic count for B. cereus from PEMBA plates incubated at between 10 and 18 o C for 6 days was observed to be about 1.0 ×10 6 cfu/g and higher than values between 1.2 × 10 3 -3.5 × 10 3 cfu/g reported by [18]. The difference observed could be attributed to the variation in the temperature and total period of incubation as opposed to the current experiment where PEMBA plates were incubated at 30ºC for a one-week period before colonies were counted.…”

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confidence: 64%

“…The PCR method used was a slightly modified version of that adopted by [15,16] using primer M13 (50-GAGGGTGGCGGCTCT-30) as according to [17]. To sterile UV irradiated Eppendorf tubes, 1 mL of the original stomached rice sample devoid of debris and 1 mL each of the sample dilutions prepared in Experiment 1 above was transferred and tubes were centrifuged at the maximum speed for 5 min on a bench microfuge (MSE Micro Centaur, Ralston Scientific / Analytical Services, Larnarkshire, UK) so as to pellet the bacteria.…”

Section: Direct Pcr For Hblementioning

confidence: 99%

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Preliminary Studies on the Detection of Bacillus cereus and Its Toxins: Comparing Conventional and Immunological Assays with a Direct Polymerase Chain Reaction Method

Abel

2019

CJAST

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Aims: Experiments were conducted to evaluate the specificity and rapidity of the application of conventional, immunoassay and direct Polymerase Chain Reaction (dPCR) techniques in the detection of Bacillus cereus and its toxins in contaminated rice. Place and Duration of Study: Department of Life Sciences, Glasgow Caledonian University, UK, between May 2015 and December 2015. Methodology: Conventional testing for the presence of B. cereus and associated toxins was achieved using Polymixin Egg-yolk Mannitol Agar (PEMBA) culture plates while immunoassays were conducted using a commercially available Reverse Passive Latex Agglutination (TD0950 BCET-RPLA, Oxoid, UK) kit. Direct PCR was used to detect the HBL-E gene in the food samples and the PCR amplicons were visualised after separation by gel electrophoresis. Results: Total Mean B. cereus count was recorded as 5.3 ×107 cfu / g of the rice sample from the PEMBA plate culture. The PEMBA method was the least in terms of rapidity of assay completion. Further assays such as the RPLA and dPCR assays were tested for potential in overcoming this limitation. Results from the immunological study showed that sample agglutination with sensitised latex beads was only positive up to the 10-2 dilution which is an indication of the presence of the Haemolysin BL enterotoxin (HBL-E). The PCR assay had the lowest limit of detection (5.3 ×106 cfu / g) thereby suggesting that the method has very low sensitivity for the bacteria. However, the PEMBA method was more sensitive but had highest detection limit (100 cfu / g) than the RPLA assay (5.3 ×105 cfu / g). Conclusion: Although the dPCR had the advantage of producing results within a short time, it was less sensitive to B. cereus than RPLA and PEMBA assays. A development of improved highly sensitive assay for the toxin has the potential to enhance food safety.

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contrasting

confidence: 64%

Section: Direct Pcr For Hblementioning

confidence: 99%

Preliminary Studies on the Detection of Bacillus cereus and Its Toxins: Comparing Conventional and Immunological Assays with a Direct Polymerase Chain Reaction Method

Abel

2019

CJAST

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“…Monitoring of changes in sub-populations of Lactic Acid Bacteria (LAB) starters and probiotic strains in fermented dairy products has been undertaken in a number of studies. The application of FCM assays using multiple stain combinations in the monitoring of pathogen survival in other food types (Cronin & Wilkinson, 2009;Kennedy & Wilkinson, 2017) has also provided fascinating insights into the degree of heterogeneity which develops in microbial populations during storage under various conditions. However, in many of the published studies few direct comparisons have been carried out between data obtained by FCM enumeration and that obtained by traditional plate counting.…”

Section: Immuno-fcm and Bacterial Enumerationmentioning

confidence: 99%

Flow cytometry as a potential method of measuring bacterial viability in probiotic products: A review

Wilkinson

2018

Trends in Food Science & Technology

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102

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“…Bacterial responses and physiological states are of particular interest to the food industry as bacteria in various physiological states can be incorporated into food matrices where they are exposed to rapid and dynamic changes in environmental conditions which can allow recovery and outgrowth (Cronin and Wilkinson, 2009;Doherty et al, 2010). Pianetti et al (2008) The application of staining probes for physiological evaluation of the target microorganism for certain samples also requires a detailed knowledge of the effect of the dye itself on the cell itself as well as the physiology and structure of the microorganism (Kennedy et al, 2011;Shi et al, 2007).…”

Section: Physiology Of Microbial Pathogens and Fcm Analysismentioning

confidence: 99%

Application of Flow Cytometry to the Detection of Pathogenic Bacteria

Kennedy¹,

Wilkinson²

2017

Current Issues in Molecular Biology

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Outbreaks of infections have emphasized the necessity for rapid and economic detection methods for pathogens in samples ranging from those of clinical origin to food products during production and retail storage, and increasingly, in environmental samples. Flow cytometry (FCM) allows the rapid acquisition of multi-parametric data regarding cell populations within fluidised samples. However, the application of FCM to pathogen detection depends on the availability of specific fluorescent probes such as antibodies and RNA probes capable of detecting and isolating pathogens from these diverse samples. A particular issue for FCM methodology is the ability to recover and discriminate bacteria from the sample matrix which may pose a major technical hurdle towards accurate and sensitive analysis. This review article focuses on detection of pathogens using FCM in samples originating from food, water, environmental and clinical sources and outlines the current state of the art and potential future applications.

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The growth, physiology and toxigenic potential of Bacillus cereus in cooked rice during storage temperature abuse

Cited by 14 publications

References 31 publications

Preliminary Studies on the Detection of Bacillus cereus and Its Toxins: Comparing Conventional and Immunological Assays with a Direct Polymerase Chain Reaction Method

Preliminary Studies on the Detection of Bacillus cereus and Its Toxins: Comparing Conventional and Immunological Assays with a Direct Polymerase Chain Reaction Method

Flow cytometry as a potential method of measuring bacterial viability in probiotic products: A review

Application of Flow Cytometry to the Detection of Pathogenic Bacteria

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