The growth of follicles in the rat ovary under the influence of Busulphan and Endoxan�

Burkl, W; Schiechl, H

doi:10.1007/bf00225543

Cited by 17 publications

(13 citation statements)

References 20 publications

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“…They checked the histology of major internal organs of these transplanted mice, and found there were histopathological changes in the liver, pancreas, spleen, lungs, and heart, but not in the kidneys. About the effects of Bu or Cy on ovaries in animals, it was reported that treatment with Bu or Cy alone can cause loss of follicles (Hemsworth and Jackson, 1963;Burkl and Schiechl, 1978;Pelloux et al, 1988;Meirow et al, 1999;Shirota et al, 2003). Until now, there was no systematic study of the effects of the combination of Bu and Cy on major internal organs in mice, especially upon the ovaries, which seem to be the most sensitive to these two drugs.…”

Section: Introductionmentioning

confidence: 99%

Accelerated ovarian aging in mice by treatment of busulfan and cyclophosphamide

Jiang

Zhao

et al. 2013

J. Zhejiang Univ. Sci. B

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Busulfan/cyclophosphamide (Bu/Cy) conditioning regimen has been widely used to treat cancer patients, while their effects on major internal organs in females are not fully understood. We treated female mice with Bu/Cy, and examined the histopathology of major internal organs on Day 30 after the treatment. The results show that Bu/Cy treatment affected the ovaries most extensively, while it had less effect on the spleen, lungs, and kidneys, and no effect on the heart, liver, stomach, and pancreas. To better understand the effect of Bu/Cy on the ovaries, we counted follicles, and determined the levels of ovarian steroids. The Bu/Cy-treated mice showed a reduction of primordial and primary follicles (P<0.01) on Day 30 and a marked loss of follicles at all developmental stages (P<0.01) on Day 60. Plasma levels of estradiol and progesterone in Bu/Cy-treated mice decreased by 43.9% and 61.4%, respectively. Thus, there was a gradual process of follicle loss and low estradiol in Bu/Cy-treated mice; this is a profile similar to what is found in women with premature ovarian failure (POF). The Bu/Cy-treated mice may serve as a useful animal model to study the dynamics of follicle loss in women undergoing POF.

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Section: Introductionmentioning

confidence: 99%

Accelerated ovarian aging in mice by treatment of busulfan and cyclophosphamide

Jiang

Zhao

et al. 2013

J. Zhejiang Univ. Sci. B

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“…CTX has been used extensively for the treatment of different cancers and certain connective tissue and autoimmune diseases, for treatment of minimal lesion glomerulonephritis, and for the control of organ rejection after transplantation [10]. This compound has been shown to produce ovarian failure in humans [11,12] and animals [13,14].…”

Section: Introductionmentioning

confidence: 99%

Luteinizing Hormone-Releasing Hormone Agonist Inhibits Cyclophosphamide-Induced Ovarian Follicular Depletion in Rhesus Monkeys1

Ataya

Rao

Lawrence

et al. 1995

Biology of Reproduction

283

130

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Several investigators have demonstrated that LHRH agonists (LHRHa) inhibit ovarian follicular depletion induced by chemotherapeutic agents in rodents. It is not clear whether or not the same effects occur in primates. Six adult female rhesus monkeys underwent unilateral ovariectomy and were divided into two groups that received monthly injections of either Lupron depot (LHRHa) or placebo vehicle. Both groups received cyclophosphamide (CTX) injections. Weekly blood samples were assayed for FSH, estradiol and progesterone. Mean serum FSH levels significantly increased in the CTX group and significantly decreased in the LHRHa+CTX group. At the end of treatment, the remaining ovary was removed and serially sectioned, and ovarian follicle number and size were analyzed. CTX resulted in a significant reduction of nonprimordial follicles < 50 microns in diameter. The rate of loss of primordial follicles was expressed as a percentage of the original follicle count. During the treatment period, 64.6 +/- 2.8% of the total primordial follicles were lost in the CTX group compared to only 28.9 +/- 9.1% in the LHRHa+CTX group (p < 0.05). The percentage rate of decline per day was 0.120 +/- 0.012 for the CTX group compared to 0.057 +/- 0.019 (p < 0.05) for the LHRHa+CTX group. The findings indicate that LHRHa can protect the ovary against CTX-induced damage in rhesus monkeys.

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“…The oldest theory is "primary or base theory". This theory claims that many mammals have ability to produce a certain number of oocytes which is determined during the embryonic period and each oocyte is surrounded by somatic cells to form follicles (6,7). In recent years, several scientists have claimed that during reproductive age and years after it, there is no increase in the number of oocytes in the ovaries and all the oocytes develop in embryonic period (6).…”

Section: Resultsmentioning

confidence: 99%