New Facts About Ovarian Stem Cells: The Origin and the Fate

Seghinsara, Abbas Majdi; Banimohammad, Majid

doi:10.15296/ijwhr.2018.21

Cited by 3 publications

(3 citation statements)

References 51 publications

(54 reference statements)

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“…[1][2][3][4] Numerous factors affect ovulation and fertility, and it is a challenge to deal with infertility and infertility-causing diseases, such as polycystic ovary syndrome. [5][6][7] In vitro maturation (IVM) of immature or preantral ovarian follicles can be used as an accepted model in order to perceive the mechanisms that may occur during folliculogenesis and also in order to study or find out the effects of multiple factors such as endocrines, antioxidants, nutrients, vitamins, and locally acting factors on follicular development. 3 On the other hand, in vitro follicular culture systems, two-dimensional (2D) and three-dimensional (3D), not only provide optimal culture models for ovarian physiology research but also support the clinical practice to achieve competent mature oocytes for in vitro fertilization (IVF).…”

Section: Introductionmentioning

confidence: 99%

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Hesperidin improves the follicular development in 3D culture of isolated preantral ovarian follicles of mice

Shoorei

Banimohammad

Kebria

et al. 2019

Exp Biol Med (Maywood)

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In vitro follicular culture systems provide optimal culture models for research about the physiology of the ovary and support the clinical practices to achieve competent mature oocytes for in vitro fertilization. In vitro maturation of preantral follicles makes it possible to study the effects of therapeutic agents on various conditions or disorders of the ovary. Nowadays, preventive bioflavonoids against cancer, hypercholesterolemia, fatty liver, or a variety of toxic agents are in focus. The aim of this study was to design and investigate the impacts of different concentrations of hesperidin, a glycoside flavonoid, on the in vitro preantral follicle growth and maturation in the three-dimensional (3D) culture system which was made with sodium alginate. Preantral follicles (n = 1363) were mechanically isolated from immature mice ovaries, then, after capsulating, they were randomly divided into four groups: the control group received no concentration of hesperidin, and three experimental groups were supplemented with 10, 22.5, and 50 µmol/L of hesperidin. All groups were cultured for 12 days. At the end of the culture period, the percentage of survival rate, antrum formation, obtained metaphase II oocytes, and the secretion of 17β-estradiol and progesterone were significantly higher in the group Hesp 50 (50 µmol/L hesperidin). Moreover, the mean average of follicular diameter cultured in the group Hesp 50 was also increased and the mRNA expression levels of PCNA, FSH-R, and Bcl-2 genes were higher, while Bax mRNA expression was significantly reduced compared with the other groups. Follicles cultured in the presence of 50 µmol/L of hesperidin had a higher fertilization rate and embryo development. Adding hesperidin at the concentration of 50 µmol/L to the culture medium resulted in higher follicular growth and maturation and increased the rate of in vitro fertilization and embryo development. Impact statement It has been stated that hesperidin has many pharmacological effects, such as anti-inflammatory and antioxidant effects, antimicrobial activity, and anti-carcinogenic activity; but hesperidin and its derivatives have been under investigation as anti-fertility factors for a very long time. However, our results show that hesperidin can improve mice follicular growth and maturation during in vitro 3D culture. Hesperidin as an antioxidant factor could enhance the mRNA expression levels of two important genes involved in folliculogenesis, PCNA, and FSH-R. Our results prove for the first time that hesperidin not only has deleterious effects on follicular development but can also increase rates of in vitro fertilization and embryo development.

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Section: Introductionmentioning

confidence: 99%

“…1–4 Numerous factors affect ovulation and fertility, and it is a challenge to deal with infertility and infertility-causing diseases, such as polycystic ovary syndrome. 5–7…”

Section: Introductionmentioning

confidence: 99%

Hesperidin improves the follicular development in 3D culture of isolated preantral ovarian follicles of mice

Shoorei

Banimohammad

Kebria

et al. 2019

Exp Biol Med (Maywood)

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“…Ovaryum yüzey epitelinin farklılaşma potansiyeli son yıllarda tartışmalı bir alan olmuş, overyan kök hücre (OKH) varlığından söz edilmeye başlanmıştır. Daha sonra birçok farklı görüş olmasına rağmen postnatal memeli ovaryumunda yeni oosit yapımının (neoogenez) olup olmadığı konusu belirsizliğini sürdürmüştür 2,6 .…”

Section: Introductionunclassified

Does the ovarian surface epithelium differentiate into primordial follicle and primary follicle precursor structures?

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2022

Cukurova Medical Journal

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Purpose: The aim of this study is to investigate the differentiation capacity of ovarian surface epithelial cells both in cell culture conditions and in ovarian tissue sections. Materials and Methods: The ovaries of two prepubertal (4 weeks old) female rats were divided into small pieces and explant cell culture was created. Ovarian surface epithelium proliferating together with ovarian stromal cells in mixed cell culture was isolated and reproduced. In addition, ovarian surface epithelium was examined in histological sections of ovarian tissue and images were taken under the microscope. Results: The morphological appearance of the ovarian surface epithelium was found to be cobblestone. In the count performed under phase contrast microscopy, it was observed that 2x106 and 3x106 cells were grown in the culture dishes, respectively. Primordial follicle-like structures were observed in some areas of the petri dishes. On the histological sections, primordial and primary follicle precursor structures were observed on the basement membrane. Conclusion: Showing oocyte markers (Gdf-9, C-Mos, Zpc, Stella) and germ cell markers (Dazl, Vasa, Blimp1, Fragilis) both in cell cultures and in histological sections can give us valuable information in terms of monitoring the differentiation capacity of these cells.

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