The growth-inhibition effect of tamoxifen in the combination chemotherapeutics on the human cholangiocarcinoma cell line QBC939

Liu, Zhihua; He, Ying; Qin, Huanlong

doi:10.1007/s11033-009-9801-2

Cited by 12 publications

(6 citation statements)

References 29 publications

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“…In addition, we identified that the expression level of miR-122 in QBC939 was significantly lower than those of HCCC-9810 and RBE cells, while there was no significant difference between HCCC-9810 and RBE. These results supported that QBC939 cells were promising for further in vitro experiment; the reason might be that QBC939 cells widely exist in metastatic foci in liver of extrahepatic bile duct carcinoma, while HCCC-9810 and RBE cells mainly exist in the primary foci in the liver, so QBC939 had a higher degree of malignancy 31 .…”

Section: Discussionsupporting

confidence: 57%

The Roles of MicroRNA-122 Overexpression in Inhibiting Proliferation and Invasion and Stimulating Apoptosis of Human Cholangiocarcinoma Cells

Liu

Jiang

et al. 2015

Sci Rep

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Our study investigated whether microRNA-122 (miR-122) played important roles in the proliferation, invasion and apoptosis of human cholangiocarcinoma (CC) cells. QBC939 and RBE cells lines were chosen and divided into five groups: miR-122 mimic group, anti-miR-122 group, negative control (NC) group, mock group and blank group. MiR-122 expression was measured by qRT-PCR. Roles of miR-122 in cell proliferation, apoptosis and invasion were investigated using MTT assay, flow cytometer and Transwell invasion assay, respectively. MiR-122 expression was lower in CC tissues and QBC939 cell than that in normal bile duct tissues, HCCC-9810 and RBE cells. In both QBC939 and RBE cells lines, miR-122 expression was higher in miR-122 mimic group than that in NC group, mock group and blank group; opposite results were found in anti-miR-122 group. Cell proliferation and invasion were remarkably inhibited in miR-122 mimic group after 48 h/72 h transfection, while apoptotic cells numbers were much greater in miR-122 mimic group; the opposite results were obtained from antimiR-122 group (all P < 0.05). MiR-122 expression was significantly weaker in CC tissues, and miR-122 overexpression might play pivotal roles in inhibiting proliferation, stimulating apoptosis and suppressing invasion of CC cells, suggesting a new target for CC diagnosis and treatment.Cholangiocarcinoma (CC) is the second commonest primary liver malignancy after hepatocellular carcinoma (HCC) and is generally classified into three forms including intrahepatic cholangiocarcinoma (ICC), hilar cholangiocarcinoma and extrahepatic cholangiocarcinoma (ECC) 1 . CC is defined as a rare and fatal tumor, and the main challenge for treating CC is considered to be the accurate diagnosis at the early stage 2 . Incidence rate and mortality rate of ICC have steadily increased over the world in the past few decades while those of ECC decreased 3 . CC widely occurs in different regions with the highest in Southeast Asia and the lowest in Australia, and the rates are 96 per 100,000 in man and 38 per 100,000 in women 4 . The incidence rate is not controlled by improvements in diagnosis because the clinical characteristics of this cancer is not easy to identify 5 . Patients with respectable CC are usually recommended

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Section: Discussionsupporting

confidence: 57%

The Roles of MicroRNA-122 Overexpression in Inhibiting Proliferation and Invasion and Stimulating Apoptosis of Human Cholangiocarcinoma Cells

Liu

Jiang

et al. 2015

Sci Rep

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“…It is widely accepted that many antitumor agents influence growth inhibition effect on malignant cells through inducing apoptosis, which is characterized with DNA fragmentation and loss of plasma membrane asymmetry (22)(23)(24). In the present study, transfection of miR-451 mimics promoted significantly DNA fragmentation and morphological changes in HCC cells.…”

Section: Discussionsupporting

confidence: 51%

miR-451: Potential role as tumor suppressor of human hepatoma cell growth and invasion

Liu

Zhang

Xiang

et al. 2014

International Journal of Oncology

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Malignant hepatoma is the leading cause of morbidity and mortality in primary liver cancer. MicroRNAs are widely accepted to act as tumor regulators to mediate tumorigenesis. Recently, miRNA-451 (miR-451) has attracted increasing attention due to its critical roles in the development of several types of cancers. Unfortunately, its function and underlying mechanism(s) of action in hepatoma remain unclear. In this study, a significant downregulation of miR-451 was observed in hepatoma cell lines. Its overexpression by administration of miR-451 mimics decreased cell viability and promoted cell apoptosis, indicating a critical role of miR-451 in cell growth. Further mechanistic analysis suggested that miR-451 overexpression accelerated cell death in a caspase-3-dependent manner, as pretreatment with its inhibitor z-VAD-fmk notably attenuated miR-451-induced cell apoptotic rates. Moreover, miR-451 upregulation abrogated cell invasive ability, accompanied with the decrease of matrix metalloproteinase-9 (MMP-9) expression levels, which may contribute to miR-451-triggered cell apoptosis. Taken together, these results reveal a prominent role of miR-451 as a tumor suppressor regulating hepatoma cell growth and invasion in a caspase-3- and MMP-9-dependent manner. Thus, our research supports this promising therapeutic agent against hepatoma.

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“…Above results agreed with previous reports on MDR reversion by SERM, such as Tamoxifen, Toremifene and Droloxifene. 8,9,[38][39][40] Our results also suggested that raloxifene could reverse MDR independent of antagonizing ER mediated signal pathway. P-glycoprotein is overexpressed in many multidrug resistant cells as an energy-dependent membrane transporter, including Bats-72 and Bads-200 cells.…”

Section: Discussionmentioning

confidence: 61%

Reversal effects of Raloxifene on paclitaxel resistance in 2 MDR breast cancer cells

Lei

Jiang

et al. 2015

Cancer Biology & Therapy

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Raloxifene hydrochloride (RAL), one of second generation of selective estrogen receptor modulators (SERMs), is usually used in preventing osteoporosis and breast cancer. The present study evaluated whether Raloxifene might sensitize multidrug resistant (MDR) breast cancers to chemotherapies, especially in estrogen receptor negative (ER-) breast cancer. The results showed that RAL could significantly sensitize ER- MDR breast tumors to paclitaxel both in vitro and in vivo. Combination of Raloxifene could significantly enhance paclitaxel-induced cell apoptosis, G2-M arrest as well as inhibition of cell proliferation in MDR tumors. Further studies showed that the combined treatment did not alter P-glycoprotein expression but increased P-gp ATPase activity. These results suggested that raloxifene might be a valuable chemosensitizer agent for breast cancer therapy.

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The growth-inhibition effect of tamoxifen in the combination chemotherapeutics on the human cholangiocarcinoma cell line QBC939

Cited by 12 publications

References 29 publications

The Roles of MicroRNA-122 Overexpression in Inhibiting Proliferation and Invasion and Stimulating Apoptosis of Human Cholangiocarcinoma Cells

The Roles of MicroRNA-122 Overexpression in Inhibiting Proliferation and Invasion and Stimulating Apoptosis of Human Cholangiocarcinoma Cells

miR-451: Potential role as tumor suppressor of human hepatoma cell growth and invasion

Reversal effects of Raloxifene on paclitaxel resistance in 2 MDR breast cancer cells

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