The gonadotropin receptors FSH-R and LH-R of Atlantic halibut (Hippoglossus hippoglossus)—2. Differential follicle expression and asynchronous oogenesis

Kobayashi, Tamae; Pakarinen, Pirjo; Torgersen, Jacob; Huhtaniemi, Ilpo; Andersen, Øivind

doi:10.1016/j.ygcen.2008.02.010

Cited by 46 publications

(23 citation statements)

References 42 publications

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“…The observation that in isolated mature ovarian follicles, lhcgrba expression is very low when compared to that in vitellogenic follicles indicates than in the mature ovary, the increased lhcgrba transcripts come from follicles at late vitellogenesis. Although we are still lacking information about the circulating levels of Fsh and Lh in Senegalese sole, these observations agree with those reported for other teleosts with group-synchronous ovaries [18,24,25,27,85], and they seem to support the concept proposed in salmonids for the roles of Fsh and Lh during the ovarian cycle [8,11].…”

Section: Discussionsupporting

confidence: 89%

“…In the present study, we cloned the full-length cDNAs for both fshra and lhcgrba in the Senegalese sole, which represents the second Pleuronectiform species in addition to Atlantic halibut [26,27], with both receptors cloned and characterized.…”

Section: Discussionmentioning

confidence: 99%

“…Sole Lhcgrba also displays greater ligandspecificity compared to Fshra. By contrast, Fsh has been reported to activate Atlantic halibut Lhcgrbb and rainbow trout Lhcgrba [22,27]. However, Sambroni et al [22] discussed that rainbow trout Lhcgrba responds only to supraphysiological doses of Fsh, such that trout Lhcgrba most likely has a strong preference for Lh.…”

Section: Discussionmentioning

confidence: 99%

“…In Teleostei, cDNAs encoding two distinct gonadotropin receptors, Fshr and Lhcgr, have been identified within a broad range of species, including members of the Elopomorpha [13], Ostariophysi [14][15][16][17][18], Protacanthopterygii [19][20][21][22][23], Paracanthoptherygii [24], and Acanthopterygii [25][26][27][28]. In each case, the overall structural homology to mammalian counterparts was conserved.…”

Section: Introductionmentioning

confidence: 99%

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Functional and Evolutionary Analysis of Flatfish Gonadotropin Receptors Reveals Cladal- and Lineage-Level Divergence of the Teleost Glycoprotein Receptor Family1

Chauvigné

Tingaud‐Sequeira

Agulleiro

et al. 2010

Biology of Reproduction

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Pituitary gonadotropins, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) act via their cognate glycoprotein hormone receptors (GpHRs), FSH receptor (FSHR), and LH/choriogonadotropin receptor (LHCGR) to regulate gonad physiology. Here, we show that the flatfish Senegalese sole (Solea senegalensis) expresses functional isoforms of fshr and lhcgr, but the genomic origin, ligand activation, and tissue distribution of the receptor transcripts are more complex than expected. By integrating the molecular phylogeny of GpHRs with the syntenic loci of vertebrate orthologs, and by subsequently characterizing the physical maps with the phylogeny of flanking genes, we found that vertebrate GpHRs have undergone a divergent evolution. In Teleostei, fshr genes have a common descent and can be classified as fshra, whereas lhcgrb genes exist as alternatively coded genes even in closely related species. Structural analyses of the receptors revealed that Fshra has an elongated ligand-binding domain, containing an extra leucine-rich repeat that specifically arose in the Acanthomorpha because of exon duplication. Ectopic expression in Xenopus laevis oocytes demonstrated that sole Fshra responded to piscine Fsh and Lh, whereas Lhcgrba was preferentially activated by its cognate hormone. The expression pattern of sole fshra and lhcgrba in gonads during the reproductive cycle was consistent with earlier observations wherein Fshra regulates ovarian growth and spermatogenesis and Lhcgrb triggers gamete maturation, respectively. However, contrary to observations in other teleosts, fshra was localized exclusively in Sertoli cells of the testis, whereas lhcgrba was expressed in Leydig cells as well as in spermatids. These results demonstrate the presence of alternatively coded lhcgr isoforms (lhcgrba and lhcgrbb) in teleosts and suggest a role of the lhcgrba receptor in the differentiation of spermatids into spermatozoa in Senegalese sole.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Functional and Evolutionary Analysis of Flatfish Gonadotropin Receptors Reveals Cladal- and Lineage-Level Divergence of the Teleost Glycoprotein Receptor Family1

Chauvigné

Tingaud‐Sequeira

Agulleiro

et al. 2010

Biology of Reproduction

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“…Therefore, information on the molecular and biochemical characterization, and regulation of gene expression is essential to fully understanding the differential roles of Gths. Due to recent considerable attention, cDNAs encoding Gth receptors (Gthrs) have been cloned in several species of teleosts (Oba et al, 1999;Bogerd et al, 2001;Kumar et al, 2001a;Maugars and Schmitz, 2006;So et al, 2005;Kobayashi et al, 2008). Molecular cloning of cDNA putatively encoding fshr and lhr in Japanese eel (Anguilla japonica) has also been reported (Ohta et al, 2007;Jeng et al, 2007) although lhr cDNA reported was a partial sequence (Jeng et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Gene Expression of Japanese Eel (Anguilla japonica) Gonadotropin Receptors

et al. 2012

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A luteinizing hormone receptor (lhr) cDNA with high identity to other fish lhrs was fully cloned from the ovary of the Japanese eel (Anguilla japonica). The genes for two gonadotropin receptors (Gthr), follicle-stimulating hormone receptor (fshr) and lhr, were differentially expressed during oogenesis, which was artificially induced by salmon pituitary extract, a gonadotropin-rich source. Transcript abundance of fshr was significantly elevated at the early vitellogenic stage and peaked at the late vitellogenic stage, while lhr gene expression rapidly induced at the late vitellogenic stage and thereafter remained at a high level. The abundance of fshr and lhr transcripts was highest in the ovary in female eels. In addition to the ovary, forebrain was a major site for the fshr transcript, although the level did not change with reproductive status. Furthermore, it was examined how eel Gthrs were activated by two mammalian chrionic gonadtropin (CG), equine CG (eCG) and human CG (hCG), that have been used for study of fish reproduction as substitutes for homologous Gths. Both CGs specifically activated eel Lhr, but not Fshr, although the degree of effectiveness was different; thus the concentration of hCG (0.1 ng/ml) required for significant activation of Lhr was much lower than that of eCG (100 ng/ml). These data on gene expression and ligand-activation of Gthrs suggest that Fsh and Lh act differentially in the regulation of reproductive function in Japanese eel.

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Molecular cloning and expression analysis of Fshr and Lhr in relation to Fshb and Lhb subunits during the period of temperature‐dependent sex determination in pejerrey Odontesthes bonariensis

Shinoda

Miranda

Okuma

et al. 2010

Molecular Reproduction Devel

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In this study, we cloned and characterized the follicle stimulating hormone receptor (Fshr) and luteinizing hormone receptor (Lhr) cDNAs of pejerrey Odontesthes bonariensis, a species with temperature-dependent sex determination (TSD), and analyzed their expression in relation to Fshb and Lhb subunits during gonadogenesis at temperatures producing only females (17 degrees C, FPT), both sexes (25 degrees C, MixPT), and only males (29 degrees C, MPT). The pejerrey Fshr cDNA had 3,069 bp for a mature protein of 694 amino acids (aa) and a signal peptide of 22 aa; the Lhr cDNA had 2,936 bp for a mature protein of 676 aa and a signal peptide of 25 aa. With the exception of Lhr in fish at the MPT, all genes showed significant increases and/or peaks of expression before histological differentiation of the gonads regardless of temperature. Larvae at the FPT had lower Fshb and Lhb but higher Lhr expression during the TSD period than those at the MPT; a clear pattern could not be ascertained for Fshr. At the MixPT, Fshb, Lhb, and Lhr mRNA increased in approximately half of the fish during TSD and sex differentiation and the sex ratio was 55.2% male. Based on the above results, it is suggested that animals with high Fshb and Lhb and low Lhr values represent putative males. These evidences, together with other studies, suggest that temperature may signal through the pituitary (differential expression of Fshb and Lhb) down to the gonads (differential expression of Lhr), probably affecting the regulation of steroidogenesis during the TSD process of pejerrey.

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The gonadotropin receptors FSH-R and LH-R of Atlantic halibut (Hippoglossus hippoglossus)—2. Differential follicle expression and asynchronous oogenesis

Cited by 46 publications

References 42 publications

Functional and Evolutionary Analysis of Flatfish Gonadotropin Receptors Reveals Cladal- and Lineage-Level Divergence of the Teleost Glycoprotein Receptor Family1

Functional and Evolutionary Analysis of Flatfish Gonadotropin Receptors Reveals Cladal- and Lineage-Level Divergence of the Teleost Glycoprotein Receptor Family1

Molecular Characterization and Gene Expression of Japanese Eel (Anguilla japonica) Gonadotropin Receptors

Molecular cloning and expression analysis of Fshr and Lhr in relation to Fshb and Lhb subunits during the period of temperature‐dependent sex determination in pejerrey Odontesthes bonariensis

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