The Golgi Apparatus of the Ameloblast of the Rat at the Stage of Enamel Matrix Formation

Kallenbach, Ernst; Sandborn, Edmund B.; Warshawsky, H.

doi:10.1083/jcb.16.3.629

Cited by 33 publications

(18 citation statements)

References 5 publications

(3 reference statements)

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“…Therefore rat enamel proteins are highly possible candidates for the MPA-and WGAbinding glycoconjugates. This view is reinforced with our findings that the distal cytoplasm of secretory ameloblasts was also stained with MPA-F and WGA-R. That is because secretory ameloblasts synthesize and secrete enamel proteins as a main product, and because they have well-developed rough-endoplasmic retioulum and Golgi apparatus in their distal cytoplasm where enamel proteins are produced (Reith 1960(Reith , 1961Kallenbach et al 1963 ;Warshawsky 1968 ;Weinstock and Leblond 1971). Previous reports conflicts on whether or not immature enamel contains galatose residues (Seyer and Glimcher 1969 ;Fukae 1970b ;Elwood and Apostolopoulos 1975b).…”

Section: Discussionsupporting

confidence: 68%

A histochemical study on lectin binding in the immature enamel and secretory ameloblasts of rat incisors.

Akita

Kobayashi

Kagayama

1988

Tohoku J. Exp. Med.

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Section: Discussionsupporting

confidence: 68%

A histochemical study on lectin binding in the immature enamel and secretory ameloblasts of rat incisors.

Akita

Kobayashi

Kagayama

1988

Tohoku J. Exp. Med.

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“…The functional roles of secretory ameloblasts in the synthesis and release of the enamel matrix precursor and the control of mineralization have been reported in previous papers [Frank, 1970;Nagai and Frank, 1975;Warshawsky and Vugman, 1977;Eisenmann et al, 1979;Ozawa et al, 1979]. Some researchers have suggested that secretory ameloblasts are involved in the resorption of organic mate rials from the enamel matrix [Kallenbach et al, 1963;Garant and Nalbandian, 1968;Holt, 1969, 1972;Kallen bach, 1977Kallen bach, , 1980Smith, 1979;Skobeet al, 1981]. A great deal of morphological evi dence suggests a resorptive function for the secretory ameloblasts: presence of many coated vesicles, pinocytotic vesicles, lyso somes, extensive GERL system, and tubular structures at the cell surface of the Tomes' process.…”

Section: Introductionmentioning

confidence: 84%

Ultrastructural and Cytochemical Studies of Resorptive and Digestive Functions of Secretory Ameloblasts in Kitten Tooth Germs

Sasaki¹

1983

Cells Tissues Organs

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Ultrastructural and cytochemical studies of kitten secretory ameloblasts were made in order to clarify their functions in the resorption and digestion of extracellular organic materials. The secretory ameloblast had triangular Tomes’ processes whose profile was divided into type 1 and type 2 faces. Type 1 face was associated with tubular structures, coated pits, coated vesicles, and irregularly shaped vesicles presumably representing phago-somes. Freeze-fracture replicas clearly showed the presence of large, particle-rich depressions and small depressions on the cell membrane P face in the type 1 face of the Tomes’ process. Exocytosis of secretory granules was seldom observed. In both thin sections and replicas, the type 2 face possessed cell membrane microinvaginations. From the supranuclear region to a zone near the Tomes’ process, many dense bodies, multivesicular bodies, and vacuoles were present; and many of them showed intense acid phosphatase reactions. Reaction products of acid phosphatase were demonstrated in the Golgi apparatus, GERL, and the lateral cell membrane. These results suggest that kitten secretory ameloblasts resorb and digest extracellular organic materials.

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“…The infranuclear cytoplasm houses mast of the ceilla mitochondria (Watson and Avery, 1954), sdme rER, and the proxiIMI junctional complex and ceU web (Ronnholm, 1962). The supranuclear r.j cytoplasm contains much rER orientated paraI leI ta the long axis of the cell and an extensive tubular-shaped Golgi apparatus (Kallenbach et al, 1963). Smooth membrane vesicles, coated veslcles, secretion granules, lysosomes, and a few profiles of rough and smooth ER are présent in the Bupranuc lear cytoplasm (Warshawsky, 1968).…”

Section: The Enamel Organ In the Secretion Zonementioning

confidence: 99%

In vivo experimentation on rat incisor enamel organs through a surgical window

McKee

Warshawsky

1984

Anat. Rec.

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Experimental agents administered systemically are costly and often toxic to animals. An in vivo technique has been developed whereby a surgical window in the alveolar bone allows selected areas of the rat incisor enamel organ and underlying enamel to be exposed to various drugs, radiolabeled molecules, and molecular weight markers. Sherman rats weighing 100 gm were anesthetized and the inferior surface of each hemimandible was surgically exposed. A slow‐speed dental hand drill was used to drill a small hole through the alveolar bone overlying the secretion or maturation zones of the enamel organ. The wound was closed and during recovery the mechanical trauma to the underlying tissue moved away from the hole due to the continuous eruption of the tooth. Two to 5 days later the hole was reexposed and microinjections of 3H‐proline, 125I‐salmon calcitonin, vinblastine sulphate, and normal saline (as control) were administered through the hole with a micro‐manipulator and a microliter syringe. Radioautographic detection of 3H‐proline incorporation in secretory ameloblasts and enamel at 10 minutes, 30 minutes, 1 hour, 4 hours, 1 day, and 2 days after microinjection was identical to that obtained previously by systemic injection. Two hours after microinjection of vinblastine sulphate the cellular response was again identical to that following systemic injection; 125I‐salmon calcitonin (M.W. ∼ 3,600D) was used as a molecular weight marker and was seen to diffuse into the enamel of the maturation zone at 10 minutes after microinjection. This study has demonstrated the feasibility of this new technique for experimentation on rat incisor enamel organs.

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The Golgi Apparatus of the Ameloblast of the Rat at the Stage of Enamel Matrix Formation

Cited by 33 publications

References 5 publications

A histochemical study on lectin binding in the immature enamel and secretory ameloblasts of rat incisors.

A histochemical study on lectin binding in the immature enamel and secretory ameloblasts of rat incisors.

Ultrastructural and Cytochemical Studies of Resorptive and Digestive Functions of Secretory Ameloblasts in Kitten Tooth Germs

In vivo experimentation on rat incisor enamel organs through a surgical window

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