A histochemical study on lectin binding in the immature enamel and secretory ameloblasts of rat incisors.

Lectin cytochemistry on the stratum intermedium and the papillary layer in the rat incisor enamel organ.

“…In this study and the observations using the HID-TCH-SP method (KOGAYA and FURUHASHI,1988) and lectin histochemistry (NAKAI et al, 1985;AKITA et al, 1988), the plasma membranes of stratum intermedium cells were revealed to be highly covered with glycocalyx. The glycocalyx of stratum intermedium cells would bear a net negative charge on their plasma membranes trapping cations (FARACH-CARSON et al, 1989) and/or positively charged organic materials.…”

“…Lectin has sometimes been used in the enamel organ (NAKAI et al, 1985;AKITA et al, 1988;NANCI et al, 1989), but the available reports fail to discuss in detail both the process of sugar incorporation as well as the role of glycocalyx.…”

Immunolocalization of CD44 and Heparan Sulfate Chains on the Stratum Intermedium and Papillary Layer in the Rat Enamel Organ.

“…Histochemistry (KOGAYA and FURUHASHI, 1988). and lectin histochemistry (NAKAI et al, 1985;AKITA et al, 1988;NAKAMURA andOZAWA, 1989, 1990) showed that the stratum intermedium and papillary layer cells are engaged in an active sugar metabolism (NAKAMURA andOZAWA, 1989, 1990). In this study, we revealed that the plasma membrane of stratum intermedium cells and papillary layer cells contains HS chains, as suggested by HID-TCH-SP method (KOGAYA and FURUHASHI, 1988) or biochemistry (GALBRAITH et al, 1992).…”

Subperiosteal lmplantation of octacalcium phosphate (OCP) stimulates both chondrogenesis and osteogenesis in the tibia, but only osteogenesis in the parietal bone of a rat

“…Wash in PBS, mount in a modified polyvinyl alcohol (PVA) medium (Lennette, 1978;Akita et al, 1988), and observe with a fluorescent microscope (Olympus BH2-ftFK).…”