Lectin cytochemistry on the stratum intermedium and the papillary layer in the rat incisor enamel organ.

Nakamura, Hiroaki; Ozawa, Hiroki

doi:10.1679/aohc.53.351

Cited by 13 publications

(12 citation statements)

References 37 publications

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“…The temporal expression in all phases of dentinogenesis and different spatial expression mainly observed in the membrane glycogonjugates founded of UEA-I ligands are in agreement with literature results (Nakai et al;Nakamura & Ozawa;Lemus et al, 1997;Dan et al, 1997;Jowett et al, 1992). Our results suggest that L-fucose expression in dentinogenesis is similar between human and rodents models and we hipotyze that this monosaccharide was intensily required on dentinogenesis.…”

Section: Iglesias D P P; Rego M J B M; Beltrão E I C and Ssupporting

confidence: 92%

CONA and UEA-I Ligands During Tooth Development Reveals Differences between Human and Rodents Models and Shows the Beginning of Mineral Extracelular Matrix Secretion

et al. 2012

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SUMMARY:Many of Glycobyological aspects of human development remain still unclear, mainly in oral science, and this could be observed in a lack of literature with few and old papers about this subject. During tooth histo-morphogenesis changes occur in basement membrane composition, expression of signaling molecules and in localization of cell surface components, where glyco components could be involved. In this sense, this work aimed to analyze the ConA ligands commonly founded in glycoproteins cores and UEA-I ligands since fucolization is a Key event in many physiological and pathological processes. Therefore 15 jaws of human fetuses were ethically obtained, histologicaly processed and then submitted to lectin histochemistry with appropriated controls. The results showed that lectins staining increase their intensity during dental development; ConA only starts to recognize glucose/mannose residues on ectomesechymal cells in the crow phase revealing its ligands when the enamel matrix starts to be secreted. Interestingly, Con A ligands were not founded in the basement membrane of the stratum intermedium of the enamel differing from rodents models. The staining pattern of UEA-I was different, starting to be positive in the ectomesenchyma since the bud stage and shown variable expression in cell type and staining intensity, which appeared be directly proportional to the progress of odontogenesis. Thus, this work shows that Con A and UEA-I exhibit a growing staining directly proporcional to ameloblasts and odontoblasts cytodiferenciation and revels some glycan differences between human odontogenesis and rodents models.

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Section: Iglesias D P P; Rego M J B M; Beltrão E I C and Ssupporting

confidence: 92%

CONA and UEA-I Ligands During Tooth Development Reveals Differences between Human and Rodents Models and Shows the Beginning of Mineral Extracelular Matrix Secretion

et al. 2012

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“…The stratum intermedium is an epithelial cell layer located between the inner enamel epithelium and stellate reticulum, but its function remains largely unknown. It has been reported that the stratum intermedium produces Shh (11) and participates in the differentiation of ameloblasts (18). Our data further revealed that signaling via IGF-1/IGF-1R is present there and it may affect the differentiation of the adjacent ameloblast layer.…”

Section: Fig 5 Expression Of Igf-1 Igf-2 Igfbp3supporting

confidence: 77%

A systematic analysis for localization of predominant growth factors and their receptors involved in murine tooth germ differentiation using in situ hybridization technique

Hisamoto¹,

Goto²,

Muto³

et al. 2015

Biomed. Res.

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Tooth development is regulated by various growth factors and their receptors. However, the overall mechanism of growth factor-mediated odontogenesis remains to be elucidated. The present study examined expression sites and intensities of major growth factors and receptors in the tooth germ of murine fetuses and neonates. Signals of TGF-β and CTGF in fetuses were released from the enamel epithelium, while their neonatal signals arose in odontoblasts. Moreover, BMP/Smad signaling may affect the differentiation of ameloblasts, in contrast to PDGFα whose signals may cause odontoblast differentiation. Growth factors associated with the formation of the periodontium were IGF1, IGF2, IGFBP3, CTGF, and PDGFα. Concerning cusp formation, the enamel knot selectively expressed FGF4, BMP2, and BMP4 with an expression of PDGFα in the enamel-free area. It is concluded that many molecules play critical roles in the epithelium-mesenchyme interaction of tooth germ differentiation, and their expressions are precisely controlled.Tooth germ differentiation commonly progresses in the following order: the initiation, bud, cap, bell, and root formation stages. According to the stages, progenitor constituents of the tooth germ proliferate and are differentiated to form specialized parts of teeth and associated tissues. Tooth development is precisely regulated by cross-talk between adjacent tissues and various growth factors secreted in autocrine and paracrine modes. Recent studies in this research field have identified many molecules which play important roles in signaling associated with tooth germ differentiation, but it is largely unknown which growth factors are the main contributors to odontogenesis, partially due to insufficient in vivo systematic analyses of the expression of such factors. The transforming growth factor-β (TGF-β) superfamily may take a leading role in the regulation of cell proliferation, differentiation, and apoptosis, also being one of the major signals for tooth and periodontium morphogenesis. Immunohistochemical studies of the mouse tooth germ detected TGF-β1 in the inner enamel epithelium, ameloblasts, and odontoblasts; the expression of TGF-β receptor-1 (TGF-βR1) was weak in the enamel epithelium but increased in intensity in differentiated ameloblasts (6). An in situ hybridization study using mouse embryos identified an intense mRNA expression of TGF-β2/3 in the odontoblast layer (20). Among bone morphogenetic proteins (BMPs) which belong to the TGF-β family, it has been reported that the expression sites of BMP2 and BMP4 mRNA in the mouse tooth germ shift from the enamel knot to dental papilla and odontoblasts, and their signalings are required for the epithelium-mesenchyme interac-

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“…Thus, it appears that the cells of SI are engaged in calcium regulation by the enzyme actions of Ca 2ϩ -ATPase and Na ϩ -K ϩ -ATPase, and play an indirect role in mineral transport to the growing enamel, coupled with the secretory ameloblasts. Based on findings with lectin cytochemistry of rat incisor enamel organ, Nakamura and Ozawa (1990) suggested that Ca ions may be trapped on the plasma membranes of these cells by highly negatively charged glycocalyx and that these cells play a role in ion translocation from the capillary to the secretory ameloblasts. CO-reactive mitochondria in the cells of SI are probably related to oxidative phosphorylation required for mineral transport.…”

Section: Discussionmentioning

confidence: 99%

Cytochrome oxidase activity in the enamel organ during amelogenesis in rat incisors

1998

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Cytochrome oxidase (CO), one of the membrane-bound mitochondrial enzymes involved in oxidative phosphorylation, reflects the functional activity of mitochondria. Mitochondria in the enamel organ show drastic changes in localization during amelogenesis (Smith. INSERM, 1984;125:273-282). In understanding the functional aspects of the enamel organ, it is essential that one knows the exact CO activity in the respective mitochondria. The present study examines the CO activity of mitochondria in the enamel organ of rat incisors throughout the various stages of amelogenesis using light and transmission electron microscopy. CO activity was examined histochemically according to Seligman et al. (J. Cell. Biol., 1968;38:1-14) in decalcified sections of the upper and lower incisors of the rat. In the secretory stage, half of the mitochondria in the ameloblasts accumulated in the infranuclear region were reactive for CO. Both the population and CO activity of the infranuclear mitochondria of ameloblasts decreased significantly in the later stage where the enamel matrix secretion was almost complete. The CO-reactive mitochondria in the cells of the stratum intermedium (SI) gradually increased in number throughout the secretory stage. In the maturation stage, the ameloblasts contained intensively CO-reactive giant mitochondria in the proximal region and regular sized ones in the distal cytoplasm that were mostly devoid of detectable CO reactivity. The proportion of CO-reactive mitochondria in the supranuclear region and the population of mitochondria in the infranuclear regions of the smooth-ended ameloblasts were significantly higher as compared with the respective values in the ruffle-ended ameloblasts. In the late stages of enamel maturation, ameloblasts containing a large number of ferritin-filled pigment vesicles possessed numerous CO-reactive mitochondria between those vesicles in the supranuclear region, implicating an active role of the ameloblasts in iron transfer into the maturing enamel. The papillary layer cells possessed numerous intensively CO-reactive mitochondria throughout the maturation stage. A stage-related variation in the localization of CO-reactive mitochondria in the enamel organ of rat incisors was quantitatively demonstrated. It is conceivable that maturation stage ameloblasts form a functional unit with the papillary layer cells, and operate in energy-requiring events such as active ion transport to, and water and

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Lectin cytochemistry on the stratum intermedium and the papillary layer in the rat incisor enamel organ.

Cited by 13 publications

References 37 publications

CONA and UEA-I Ligands During Tooth Development Reveals Differences between Human and Rodents Models and Shows the Beginning of Mineral Extracelular Matrix Secretion

CONA and UEA-I Ligands During Tooth Development Reveals Differences between Human and Rodents Models and Shows the Beginning of Mineral Extracelular Matrix Secretion

<b>A systematic analysis for localization of predominant growth factors and their receptors involved in murine tooth germ differentiation using </b><i><b>in situ</b></i><b> hybridization </b><b>technique </b>

Cytochrome oxidase activity in the enamel organ during amelogenesis in rat incisors

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