The Golgi Apparatus in Neurons and Epithelial Cells of the Common Limpet Patella Vulgata

Abstract

1. In view of widely diverse views held about the identity and structure of the Golgi apparatus in neurons of Mollusca, particularly gastropods, a study has been made on neurons of the common limpet, Patella vulgata, both by light and electron microscopy. A report is given also of observations made on epithelial cells of Patella by electron microscopy. 2. As revealed by Kolatchev's method, the Golgi apparatus in neurons consists basically of black filaments lying to one side of the nucleus. The filaments generally anastomose to form networks of various complexity. Rarely some cells contain only discrete filaments. Associated with some of the filaments is a weakly osmiophilic substance identified as archoplasm. Kolatchev's method also revealed spheroidal bodies (neutral red bodies, "lipochondria," etc.). 3. It has not been possible to demonstrate the Golgi apparatus using either iron-haematoxylin or Sudan black. 4. Examination of Kolatchev's preparations by electron microscopy has revealed that some of the Golgi filaments consist of chromophilic and chromophobic components. The chromophilic component consists of dense lamellae. 5. After fixation in buffered osmium tetroxide solution and examination by electron microscopy, it has been concluded that (a) the chromophilic component of the Golgi apparatus corresponds to a system of paired membranes (which usually enclose an inner dense substance), (b) the chromophobic component corresponds to a substance lying within small dilations of the paired membrane, and (c) the archoplasm corresponds to numerous small vesicles. 6. The paired membranes branch, anastomose, and can often be traced back to a common source. They are interpreted as lamelliform folds, and occasionally tubular processes, of essentially a single Golgi membrane. In cells containing a Golgi network it is suggested that the membrane extends through the whole of the apparatus in such a way that the substance it encloses may be regarded as being in a continuous phase. 7. Epithelial cells of Patella contain a juxtanuclear Golgi apparatus with an ultrastructure similar to that described for neurons.

New Problems in the Functional Activity of the Pacinian Corpuscle

“…It is of interest that in this animal neighbouring bundles of flattened Golgi vesicles are sometimes connected by 344 BRIEF NOTES a few vesides. A somewhat similar condition was previously observed in the neurons of Patella ~Igata (7,9). According to Lacy (7) the Golgi lamellae of Patella branch and anastomose to form a network.…”

“…Further, it is claimed that the dictyosomes of germ cells and of somatic cells of invertebrates basically resemble in their fine structure the Golgi complex of vertebrate somatic cells (2)(3)(4)(5)(6)(7). We believe that the Golgi complex of the cells forming the wall of the small blood vessels of Helix is made up of flattened vesicles, large vacuoles, and minute vesicles, and that it, therefore, closely resembles the complex of the somatic cells of vertebrates.…”

Cell Polarity as a Regulator of Cancer Cell Behavior Plasticity

“…In polarized epithelial cells, the Golgi complex is positioned in juxtaposition to the face of the nuclei proximal to the apical membrane (Barr & Egerer 2005, Lacy 1957) (Figure 3 a ). Golgi fragmentation does not significantly affect general vesicle transport but significantly impairs polarized secretion of VSVG particles to the apical membrane (Yadav et al 2009).…”

AN ELECTRON MICROSCOPE STUDY OF SOME CYTOPLASMIC INCLUSIONS OF THE NEURONES OF THE PRAWN, LEANDER SERRATUS

“…9). The rods and crescents are commonly identified as Golgi apparatus in electron micrographs of the neurones of invertebrates (Lacy, 1957 ;Hess, 1958;compare Chou & Meek, 1958;Baker, 1959). They are presumably the objects described as "dictyosomes" in studies by light microscopy.…”