The golden mussel proteome and its response to niclosamide: Uncovering rational targets for control or elimination

Sanson, Ananda Lima; Cosenza-Contreras, Miguel; DeMarco, Ricardo; Neves, Leandro Xavier; Mattei, Bruno; Silva, Gustavo Gonçalves; Magalhães, Paulo Henrique Vieira; Andrade, Milton Hércules Guerra de; Castro-Borges, William

doi:10.1016/j.jprot.2020.103651

Cited by 7 publications

(3 citation statements)

References 42 publications

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“…Commonly used chemical oxidants include sodium hypochlorite, chloramine T, potassium permanganate, hydrogen peroxide and chlorine dioxide (Li et al, 2019). Additionally, MXD‐100, BULAB 6002, H‐130 (Didecyl Dimethyl Ammonium Chloride) and chlorfenapyr have also been tested as L. fortunei extermination agents (Moreira et al, 2023; Pereyra et al, 2011; Pereyra et al, 2012; Sanson et al, 2020). While chemical methods can achieve prolonged eradication with simple processes, they inevitably cause environmental pollution.…”

Section: Control Methods Of Limnoperna Fortuneimentioning

confidence: 99%

Distribution, tolerance, growth, behaviour and control methods of Limnoperna fortunei (Dunker, 1857) (Bivalvia: Mytilidae): A review

Liu,

He,

Yang

et al. 2024

Aquatic Conservation

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Limnoperna fortunei (L. fortunei) (Dunker, 1857), the golden mussel, is an invasive species possessing exceptional environmental adaptability and a destructive impact on water systems and artificial structures. To better conservation of freshwater ecosystems by controlling this mussel, it is essential to study its distribution, potential for invasion, environmental tolerance and biological traits. This paper outlines the distribution of L. fortunei in Asia and South America, as well as its potential for future invasion in freshwater habitats, along with the research methods employed. We find that the mussel had a temperature tolerance range of 8–35 °C and a pH range was 5–10, with some individuals surviving in extreme conditions for extended periods. We find that temperature was a decisive factor for success at reproductive stages, larval development and adult growth. The biological traits of L. fortunei are discussed, focusing on feeding habits, attachment characteristics and locomotion, with an emphasis on byssus structure, foot proteins and adhesion mechanisms. The study elucidates the impact of flow velocity, light exposure and substrate material on their attachment, with the optimal attachment being identified at a flow velocity of 0.3–0.9 m/s. Additionally, the mussel exhibits strong light avoidance performances and a preference for rough surfaces. Extensively‐researched control strategies are classified and summarized, primarily categorized into physical, chemical and biological control, with an outline of their respective strengths and limitations.

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Section: Control Methods Of Limnoperna Fortuneimentioning

confidence: 99%

Distribution, tolerance, growth, behaviour and control methods of Limnoperna fortunei (Dunker, 1857) (Bivalvia: Mytilidae): A review

Liu,

He,

Yang

et al. 2024

Aquatic Conservation

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“…Purified toxins were subjected of their N-terminus by employing the automated Edman degradation sequencing technique, using a Shimadzu PPSQ-21A (Tokyo, Japan). Subsequently, toxins were subjected to an in-solution digestion protocol was used as described by Sanson et al [ 62 ]. Briefly, samples containing 50 µg protein were solubilized in 25 mM ammonium bicarbonate (AMBIC) followed by addition of RapiGest SF Surfactant (Waters, UK) to a final concentration of 0.06% (w/v), and heated at 80 ºC for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Comparative venomic profiles of three spiders of the genus Phoneutria

Fernandes

Moraes

Santos

et al. 2022

J. Venom. Anim. Toxins incl. Trop. Dis

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Background: Spider venoms induce different physio-pharmacological effects by binding with high affinity on molecular targets, therefore being of biotechnological interest. Some of these toxins, acting on different types of ion channels, have been identified in the venom of spiders of the genus Phoneutria, mainly from P. nigriventer. In spite of the pharmaceutical potential demonstrated by P. nigriventer toxins, there is limited information on molecules from venoms of the same genus, as their toxins remain poorly characterized. Understanding this diversity and clarifying the differences in the mechanisms of action of spider toxins is of great importance for establishing their true biotechnological potential. This prompted us to compare three different venoms of the Phoneutria genus: P. nigriventer (Pn-V), P. eickstedtae (Pe-V) and P. pertyi (Pp-V). Methods: Biochemical and functional comparison of the venoms were carried out by SDS-PAGE, HPLC, mass spectrometry, enzymatic activities and electrophysiological assays (whole-cell patch clamp). Results: The employed approach revealed that all three venoms had an overall similarity in their components, with only minor differences. The presence of a high number of similar proteins was evident, particularly toxins in the mass range of ~6.0 kDa. Hyaluronidase and proteolytic activities were detected in all venoms, in addition to isoforms of the toxins Tx1 and Tx2-6. All Tx1 isoforms blocked Nav1.6 ion currents, with slight differences. Conclusion: Our findings showed that Pn-V, Pe-V and Pp-V are highly similar concerning protein composition and enzymatic activities, containing isoforms of the same toxins sharing high sequence homology, with minor modifications. However, these structural and functional variations are very important for venom diversity. In addition, our findings will contribute to the comprehension of the molecular diversity of the venoms of the other species from Phoneutria genus, exposing their biotechnological potential as a source for searching for new active molecules.

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“…Recently label-free quantitative proteomics and isobaric tags for relative and absolute quantitation based proteomics have become important technologies, they are able to not only identify a larger number of proteins but also quantify the relative abundances of protein in various samples. Previous studies have used these technologies to determine the proteomic responses of bivalves to niclosamide, ocean acidi cation and environmental variation (Sanson et al, 2020;Quan et al, 2015;Venkataraman et al, 2019). Ionomics has been gradually applied to the discussion of the relationship between elements in organisms.…”

Section: Introductionmentioning

confidence: 99%

Effect of cadmium in the gonads of mussel (Mytilus coruscus): an ionomics and proteomics study

Meng

Wang

Zhang

et al. 2023

Environ Sci Pollut Res

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The mussel Mytilus coruscus is an important and very popular seafood in China and widespread along the eastern coast of China. In this study, we investigated the molecular response of mussel gonads to cadmium hyperaccumulation at two concentrations (80 and 200 µg/L) for 30 days using ionomics and proteomics techniques. The shrinkage of the cells and moderate hemocytic in ltration were observed in the Cd-treated groups. The strontium, selenium (Se), and zinc contents were signi cantly altered, and the relationships between iron, copper, Se, manganese, calcium, sodium, and magnesium were also signi cantly altered. Label-free quantitative proteomics analysis revealed a total of 227 differentially expressed proteins. These proteins were associated with multiple biological processes, including the tricarboxylic acid cycle, structural reorganization of cells, biosynthesis of amino acids, in ammatory response of cells and tumorigenesis. Nonetheless, our ionomics and proteomics analysis revealed that mussels could partly alleviate the adverse effects of Cd by altering the metal contents and correlations between minerals, thereby enhancing the biosynthesis of some amino acids and activity of antioxidant enzymes. Overall, this study provides insight into the mechanism underlying Cd toxicity in mussel gonads from a metal and protein perspective.

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The golden mussel proteome and its response to niclosamide: Uncovering rational targets for control or elimination

Cited by 7 publications

References 42 publications

Distribution, tolerance, growth, behaviour and control methods of Limnoperna fortunei (Dunker, 1857) (Bivalvia: Mytilidae): A review

Distribution, tolerance, growth, behaviour and control methods of Limnoperna fortunei (Dunker, 1857) (Bivalvia: Mytilidae): A review

Comparative venomic profiles of three spiders of the genus Phoneutria

Effect of cadmium in the gonads of mussel (Mytilus coruscus): an ionomics and proteomics study

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