Hesperetin is the main pharmacological ingredient of fruit of the citrus family, rutaceae. It is a flavanone, which has potent antioxidation and anti‑inflammatory activities. The present study investigated the preventive effect of hesperidin in the modulation of acute myocardial infarction (AMI)‑induced inflammatory responses and antioxidant status in a mouse model. The levels of creatine kinase‑MB, tumor necrosis factor (TNF‑α), interleukin (IL)‑1β, IL‑6, monocyte chemoattractant protein 1 (MCP‑1), intercellular adhesion molecule 1 (ICAM‑1), malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD) and caspase‑3/9 were measured using ELISA kits. Western blot analysis analyzed p53 and B‑cell lymphoma 2 (Bcl‑2)‑associated X protein/Bcl‑2, and induced the expression of peroxisome proliferator‑activated receptor‑γ (PPAR‑γ). Hesperidin markedly decreased the myocardial infarction area, heart weight/body weight ratio and activity of creatine kinase‑MB in AMI mice. Hesperidin treatment caused a significant decrease in the levels of TNF‑α, IL‑1β, IL‑6, MCP‑1, ICAM‑1, MDA, CAT, SOD and caspase‑3/9 in mice with AMI. Hesperidin also significantly suppressed the protein expression levels of p53 and Bax/Bcl‑2, and induced the expression of peroxisome proliferator‑activated receptor‑γ (PPAR‑γ) in mice with AMI. The preventive effect of hesperidin modulated the inflammatory response and antioxidant status following AMI through downregulation of the expression of PPAR‑γ and Bcl‑2 in the model mice.

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Zn‐binding protein was obtained after purification from scallops (Mizuhopecten yessoensis) using gel permeation and ion‐exchange chromatography. Amino acid determination showed that the cysteine of the zinc‐binding protein accounted for one‐third of the total amino acids, which is a typical feature of metallothionein (MT). The spectra of Fourier Transform Infrared Spectroscopy (FTIR) and Circular Dichroism (CD) were analyzed to predict the secondary structure information of zinc‐binding protein: the α‐helix was 46.55%, the β‐sheets was 27.07%, the random coil was 16.48%, and the β‐turns was 9.89%. Using a commercial kit to measure its antioxidant activity in vitro, the result showed that it had good scavenging ability to 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH), hydroxyl radical (·OH), and reducing the ability to ferrous iron ions. With the process provided by this study, zinc‐binding protein can be prepared in large quantities, which is the basis for its future commercialization. Practical applications According to the extraction and purification process established in this study, a large amount of zinc‐bound MT from the viscera of scallops can be obtained. And the zinc‐bound MT had good antioxidant activity. In addition, the yield of each purification step has been calculated. The zinc‐bound MTs from scallop’ viscera can be prepared in large quantities by directly using the process in this manuscript or by equal magnification of this process. In the future, large‐scale production can be considered to increase the economic value of scallops’ viscera.

The expression levels of micro ribonucleic acid-183 (miR-183) and miR-141 in the lesion tissues of infected abdominal aortic aneurysm (IAAA) and their relationship with prognosis were investigated. Thirty-six patients with IAAA admitted and who underwent vascular surgery in People's Hospital of Shenzhen from June 2003 to June 2013 were selected. Reverse transcription polymerase chain reaction (RT-PCR) was utilized to detect the expression levels of miR-183 and miR-141 in lesion tissues and adjacent tissues 1 cm away from the aneurysm in 36 patients with IAAA. The relationship between the expression levels of miR-183 and miR-141 as well as the clinicopathological features of patients with IAAA were analyzed, and the factors influencing the prognosis of IAAA were analyzed by univariate and multiva-riate analysis. The expression levels of miR-183 and miR-141 were significantly downregulated in the lesions of patients with IAAA, and miR-183 and miR-141 levels in the lesion tissues of the IAAA patients were significantly lower than those in the adjacent tissues (P<0.05). The expression levels of miR-183 and miR-141 were not related to sex, age, history of hypertension, and alcoholism (P>0.05), but they were related to smoking history or aneurysm size (P<0.05). The overall survival rate of patients with IAAA was 41.6% (15/36). The multivariate analysis found that aneurysm size, low expression of miR-183, and low expression of miR-141 were independent factors affecting the prognosis of patients with IAAA. In conclusion, the expression levels of miR-183 and miR-141 in the lesion tissues of IAAA are low, and the lower the expression level is, the worse the prognosis gets. miR-183 and miR-141 can be used as predictors of prognosis in patients with IAAA.