The Glycogen Content of the Human Female Genital Tract in Cycling, Menopausal, and Women with Encometrial and Cervical Carcinoma

Gregoire, A. T.; Ledger, W.D.; Morán, María

doi:10.1016/s0015-0282(16)39553-x

Cited by 15 publications

(7 citation statements)

References 13 publications

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“…Interestingly, this phenomenon only affects ectocervical cells, while in endocervical cells, changes in glycogen levels during the menstrual cycle are small, and its level after menopause is not lower than on most days of the cycle in menstruating women [43]. In turn, in the case of cervical cancer, the level of glycogen decreases in both ecto-and endocervical cells, compared to the average level in the secretory phase [43].…”

Section: Introductionmentioning

confidence: 97%

“…In the case of cervical epithelial cells, glycogen levels vary in different phases of the menstrual cycle, and after menopause it decreases significantly [43]. Interestingly, this phenomenon only affects ectocervical cells, while in endocervical cells, changes in glycogen levels during the menstrual cycle are small, and its level after menopause is not lower than on most days of the cycle in menstruating women [43]. In turn, in the case of cervical cancer, the level of glycogen decreases in both ecto-and endocervical cells, compared to the average level in the secretory phase [43].…”

Section: Introductionmentioning

confidence: 99%

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HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular Resolution

Sitarz

Czamara

Bialecka³

et al. 2020

IJMS

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Using Raman microscopy, we investigated epithelial cervical cells collected from 96 women with squamous cell carcinoma (SCC) or belonging to groups I, IIa, IIID-1 and IIID-2 according to Munich III classification (IIID-1 and IIID-2 corresponding to Bethesda LSIL and HSIL groups, respectively). All women were tested for human papillomavirus (HPV) infection using PCR. Subcellular resolution of Raman microscopy enabled to understand phenotypic differences in a heterogeneous population of cervical cells in the following groups: I/HPV−, IIa/HPV−, IIa/HPV−, LSIL/HPV−, LSIL/HPV+, HSIL/HPV−, HSIL/HPV+ and cancer cells (SCC/HPV+). We showed for the first time that the glycogen content in the cytoplasm decreased with the nucleus size of cervical cells in all studied groups apart from the cancer group. For the subpopulation of large-nucleus cells HPV infection resulted in considerable glycogen depletion compared to HPV negative cells in IIa, LSIL (for both statistical significance, ca. 45%) and HSIL (trend, 37%) groups. We hypothesize that accelerated glycogenolysis in large-nucleus cells may be associated with the increased protein metabolism for HPV positive cells. Our work underlines unique capabilities of Raman microscopy in single cell studies and demonstrate potential of Raman-based methods in HPV diagnostics.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular Resolution

Sitarz

Czamara

Bialecka³

et al. 2020

IJMS

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“…Di-to hexasaccharides were evident by FACE analysis and accounted for the majority of the total carbohydrates. It is presumed that the oligosaccharides are of the malto series derived from glycogen, since the nonkeratinized squamous epithelial cells lining the vagina are rich in glycogen (9). The in situ concentration of the glucose oligosaccharides in the vaginal fluid based on the glucose content was calculated to be between 80 and 160 mM.…”

Section: Discussionmentioning

confidence: 99%

Roles of Glycoproteins and Oligosaccharides Found in Human Vaginal Fluid in Bacterial Adherence

Rajan¹,

Cao²,

Anderson

et al. 1999

Infect Immun

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Adherence of type 1-piliated Escherichia coli to carbohydrate structures of vaginal mucosa plays a major role in the pathogenesis of ascending urinary tract infections in women. Colonization of the vaginal introitus is influenced by interactions between pathogens, vaginal fluid, and vaginal epithelium. In this study, the type and amount of carbohydrates and glycoproteins present in vaginal fluid were determined. Free and protein-bound oligosaccharides in vaginal fluid specimens were analyzed by fluorophore-assisted carbohydrate electrophoresis (FACE) and high-pressure liquid chromatography (HPLC). Two-dimensional electrophoretic separations of vaginal fluid glycoproteins were performed together with bacterial overlay assays. The results of FACE showed that the majority of the oligosaccharides are in the free state and the bound oligosaccharides are undetectable. HPLC analysis of free sugars revealed glucose as the major sugar (3.3 ؎ 0.3 mM), and the concentrations of mannose and glucosamine were 0.065 ؎ 0.04 and 0.02 ؎ 0.001 mM, respectively. Radiolabeled E. coli bound three vaginal fluid glycoproteins with the following molecular masses and pIs: 82 kDa and pI 5.5, 55 kDa and pI 4.5, and 55 kDa and pI 6.5. The binding was inhibited by mannose and by deglycosylation of the proteins prior to the overlay assay. One of these putative receptors was identified to be the heavy chain of secretory IgA (S-IgA). These data suggest that the free mannose in the fluid is less than that required to affect E. coli-epithelial cell binding interactions and that S-IgA may bind E. coli in the vaginal introitus.

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“…Failure to do so will lead to the development of poor quality of endometrium resulting in death of ovum either before or after implantation 14 . Genital tract glycogen is unique in that unlike muscular glycogen, it is unaffected by either carbohydrate intake or exercises, but is controlled by ovarian steroids 15 . Maeyama M et al found the glycogen content of the endometrium of normal and infertile patients was measured during the menstrual cycle.…”

Section: Glycogen Content Of Endometriummentioning

confidence: 99%

A Correlative Study of Endometrial Glycogen Content and Other Contributory Factors on Female Infertility

Girish¹,

Kotur²,

Nagarajappa³

et al. 2012

Int J of Biomed & Adv Res

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Background and Objective: Endometrial glycogen is one of the most important factors for development of the blastocyst in the early stages of gestation which is reconverted into a simple monosaccharide like glucose at the time of implantation. Hence in the present study, the role of glycogen content in the endometrium and other factors like age, duration of infertility pattern of menstruation and other clinical features in causing infertility was studied. Materials and Methods: This study was carried out in the Department of Pathology, Bangalore Medical College after the ethical clearance from the institutional ethics committee. The study included 90 cases with complaints of infertility (primary / secondary). The endometrial tissue was fixed; processed, sectioned and performed Periodic Acid Schiff (PAS) stain for detecting the amount of glycogen in endometrium. The present prospective study was a descriptive study and the values are mentioned in percentages. Result: We observed that, the majority of primary infertility patients were in the age group of 21-25 years and that of secondary were in 26-30 years. Most of the patients 55(74.3%) in primary & 12(75%) had regular cycles. The duration of primary infertility group was 2-3 years and that of secondary infertility group was 4-5 years. The glycogen content in the endometrium was grossly reduced in patients with Luteal Phase Defects. Conclusion: We found that the glycogen content of endometrium was very much deficient in late secretory phase might be one of the major causes for female sterility.

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The Glycogen Content of the Human Female Genital Tract in Cycling, Menopausal, and Women with Encometrial and Cervical Carcinoma

Cited by 15 publications

References 13 publications

HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular Resolution

HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular Resolution

Roles of Glycoproteins and Oligosaccharides Found in Human Vaginal Fluid in Bacterial Adherence

A Correlative Study of Endometrial Glycogen Content and Other Contributory Factors on Female Infertility

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