The Genus Phytophthora (Peronosporales) in Argentina

Palmucci, H. E.; Wolcan, S. M.

doi:10.31055/1851.2372.v55.n2.25537

Cited by 3 publications

(2 citation statements)

References 49 publications

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“…To assess the infectivity of Phytophthora cinnamomi zoospore stocks, we developed a rapid inoculation method using Solanum lycopersicum seedlings. Solanum lycopersicum has been reported as a host for P. cinnamomi [ 4 , 67 – 69 , 72 ], enabling swift evaluation of zoospore infectivity. Fourteen-day-old Solanum lycopersicum seedlings were inoculated with previously frozen zoospore stocks directly onto soil.…”

Section: Resultsmentioning

confidence: 99%

An improved method to study Phytophthora cinnamomi Rands zoospores interactions with host

Del Castillo-González,

Soudani,

De La Cruz-Gómez

et al. 2024

BMC Plant Biol

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Phytophthora cinnamomi Rands is a highly prevalent phytopathogen worldwide, ranking among the top ten in terms of distribution. It inflicts crown rot, canker, and root rot on numerous plant species, significantly impacting the biodiversity of both flora and fauna within affected environments. With a host range spanning over 5,000 species, including important plants like Quercus suber, Quercus ilex, Castanea sativa, and commercially significant crops such as avocado (Persea americana), maize (Zea mays), and tomato (Solanum lycopersicum), Phytophthora cinnamomi poses a substantial threat to agriculture and ecosystems. The efficient dissemination of the oomycete relies on its short-lived asexually motile zoospores, which depend on water currents to infect host roots. However, managing these zoospores in the laboratory has long been challenging due to the complexity of the life cycle. Current protocols involve intricate procedures, including alternating cycles of growth, drought, and flooding. Unfortunately, these artificial conditions often result in a rapid decline in virulence, necessitating additional steps to maintain infectivity during cultivation. In our research, we sought to address this challenge by investigating zoospore survival under various conditions. Our goal was to develop a stable stock of zoospores that is both easily deployable and highly infective. Through direct freezing in liquid nitrogen, we have successfully preserved their virulence. This breakthrough eliminates the need for repeated culture transfers, simplifying the process of plant inoculation. Moreover, it enables more comprehensive studies of Phytophthora cinnamomi and its interactions with host plants.

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Section: Resultsmentioning

confidence: 99%

An improved method to study Phytophthora cinnamomi Rands zoospores interactions with host

Del Castillo-González,

Soudani,

De La Cruz-Gómez

et al. 2024

BMC Plant Biol

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“…To determine whether Zs stocks retain the original capacity to infect plants, we established a rapid method for inoculating tomato seedlings with Pc's Zs. Tomato has previously been described as a host for Pc 64,65,70,71 allowing us to rapidly determine the ability of Zs to infect a plant. Fourteen day old tomato seedlings were inoculated on soil with Zs stocks of previously frozen Zs.…”

Section: Survival and Recovery Of Zs To Freezing Pointmentioning

confidence: 99%

An improved method to study Phytophthora cinnamomi Rands zoospores interactions with host

Lucía,

Serine,

Noelia

et al. 2024

Preprint

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The pathogen Phytophthora cinnamomi Rands (Pc) is one of the ten most widespread phytopathogens in the world causing crown rot, canker and root rot in multi-functional land plants. Pc has a disastrous impact on the surrounding environment and biodiversity of flora, fauna and soil. Pc infects more than 5,000 species, including Quercus suber, Quercus ilex, Castanea sativa, Eucalyptus globulus, Persea americana (avocado), Zea mays (maize) and Solanum lycopersicon (tomato). The efficient spread of Pc depends on the short survival of asexually motile zoospores (Zs), which move through water currents until they penetrate the host roots. Due to the complexity of the life cycle, the management of Zs in the laboratory has remained quite complex for decades. The production of Zs requires mimicking nature by following a complex protocol of circular steps of growth, drought and flooding. Similar to other pathogens, the artificial growth conditions induce a rapid loss of virulence, making it necessary to add additional steps of plant infection in between these other steps. In this work, the study of the survival of Zs under different conditions has allowed us to obtain a "ready-to-use" stable stock of Zs with a high capacity to infect the host by direct freezing in liquid nitrogen. Obtaining this stock prevents the loss of virulence of Pc between cultures, thus greatly simplifying the process of inoculating plants and studying Pc with their host.

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