The Genus Nocardioides

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

et al. 2008

supporting

confidence: 63%

mentioning

confidence: 99%

Nocardioides koreensis sp. nov., Nocardioides bigeumensis sp. nov. and Nocardioides agariphilus sp. nov., isolated from soil from Bigeum Island, Korea

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

et al. 2008

“…Members of the genus Nocardioides possess iso-C 16 : 0 as the major fatty acid and have DNA G+C contents in the range 67-74 mol% (Prauser, 1976(Prauser, , 1984Yoon & Park, 2006). Strain MSL-01 T possesses phenotypic characteristics typical of the genus Nocardioides.…”

mentioning

confidence: 99%

Nocardioides sediminis sp. nov., isolated from a sediment sample

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

et al. 2009

A strictly aerobic, motile, short-rod-shaped, Gram-positive-staining actinomycete strain, designated MSL-01 T , was isolated from a sediment sample from Bigeum Island of Korea. 16S rRNA gene sequence analysis revealed that the isolate MSL-01 T belonged to the genus Nocardioides, with the highest sequence similarity to Nocardioides terrigena DS-17 T (98.54 %), but the DNA-DNA relatedness to this type strain was 34 %. The physiological properties of strain MSL-01 T differ from those of Nocardioides terrigena DS-17 T and other species of Nocardioides. The diamino acid in the cell-wall peptidoglycan of strain MSL-01 T is LL-diaminopimelic acid, the major menaquinone is MK-8(H 4 ) and iso-C 16 : 0 is the major fatty acid component. The name Nocardioides sediminis sp. nov. is proposed for the novel species, with the type strain MSL-01 T (5DSM 19263 T 5KCTC 19271 T ).The genus Nocardioides was proposed by Prauser (1976) for Gram-positive-staining, non-acid-fast, aerobic and mesophilic nocardioform actinomycetes that develop a mycelium that fragments into irregular rod-coccus-like elements. Originally, the genus contained the two species Nocardioides albus and Nocardioides luteus (Prauser, 1984). The number of species belonging to the genus Nocardioides has increased considerably, revealing the morphological heterogeneity of the genus. Presently, the genus includes 28 recognized species, including the recently described species Nocardioides terrigena (Yoon et al., 2007), Nocardioides marinisabuli , Nocardioides daphniae (Tó th et al., 2008) and Nocardioides hankookensis (Yoon et al., 2008). In this study, we report the taxonomic characterization of a Nocardioides-like strain, MSL-01 T , which was isolated from a sediment sample collected on Bigeum Island, Jeollanam-do Province, Korea.Strain MSL-01 T was isolated from a sediment sample taken during May 2006 at a depth of 60-70 m. The top 10 cm of the sediment surface was obtained aseptically for sampling and then processed for cultivation experiments by using standard dilution techniques at 28 u C on tenfold-diluted R2A agar (Reasoner & Geldreich, 1985; Difco). To investigate the morphological, physiological and biochemical characteristics of strain MSL-01 T , the strain was routinely cultivated at 28 uC on twofold-diluted R2A agar and the strain was stored as glycerol suspensions (20 %, v/v) at 270 u C. Gram staining was performed using a bioMérieux Gram-stain kit according to the manufacturer's instructions. Cell morphology and motility were studied using phase-contrast and scanning electron microscopy (JEOL apparatus Philips SEM 515). The physiological characteristics of strain MSL-01 T were examined by growing the isolate on diluted R2A medium. Growth at various temperatures (4-50 u C) was measured on twofolddiluted R2A agar. Growth at different pH levels (pH 6.0-12.0) was determined after adjusting the pH of the medium by the addition of Na 2 CO 3 prior to sterilization. Growth at various NaCl concentrations (0-20 % w/v) was investigated on twofold-diluted R2A aga...

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“…Strain T exhibited characteristics of the genus Nocardioides (iso-C 16 : 0 as the major fatty acid and DNA G+C contents in the range of 67-74 mol%; Prauser, 1976Prauser, , 1984Yoon & Park, 2006).…”

Section: Strain Msl-22mentioning

confidence: 99%

Nocardioides mesophilus sp. nov., isolated from soil

International Journal of Systematic and Evolutionary Microbiology

Pandey

et al. 2010