The genus Cryptosphaeria in the western United States: taxonomy, multilocus phylogeny and a new species, C. multicontinentalis

Trouillas, Florent P.; Hand, Francesca Peduto; Inderbitzin, Patrik; Gubler, W. D.

doi:10.3852/15-115

Cited by 26 publications

(13 citation statements)

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“…and cause similar disease symptoms in these hosts (Hinds ; Vasilyeva and Ma ; Trouillas et al. ). However, Cr.…”

Section: Discussionmentioning

confidence: 85%

“…pullmanensis (Trouillas et al. ). Cryptosphaeria subcutanea is morphologically the closest taxon to Cr.…”

Section: Discussionmentioning

confidence: 99%

“…fraxini (Richon) F. Rappaz have so far been accepted in Cryptosphaeria (Vasilyeva , ; Hyde ; Romero and Carmarán ; Vasilyeva and Ma ; Trouillas et al. ). The evolutionary relationships among the members of this genus mainly remain uncertain and it is likely that dissimilar fungi have been placed under the generic name Cryptosphaeria (Rappaz ; Trouillas et al.…”

Section: Introductionmentioning

confidence: 99%

“…The evolutionary relationships among the members of this genus mainly remain uncertain and it is likely that dissimilar fungi have been placed under the generic name Cryptosphaeria (Rappaz ; Trouillas et al. ).…”

Section: Introductionmentioning

confidence: 99%

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Cryptosphaeria Canker of Populus nigra Caused by Cryptosphaeria pullmanensis, a New Threat to Poplar Industry in Iran

Aghdam

Babai-Ahari

Karimi

et al. 2017

Journal of Phytopathology

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Trunk diseases are potential threats to the poplar industry worldwide, including Iran. A survey on trunk diseases of Populus nigra in north‐western Iran revealed a new canker disease associated with dieback and decline of this host in West Azarbaijan Province of Iran. Wood samples were collected from poplar trees showing canker, dieback and decline symptoms and taken to the laboratory. A total of 173 fungal isolates were recovered from symptomatic tissues, of those 147 isolates had similar cultural and morphological features on potato dextrose agar. Based on a combination of morphological characteristics and phylogenetic inferences including DNA sequence data from the internal transcribed spacer regions (ITS1, 5.8S rDNA, and ITS2), all 147 isolates were identified as Cryptosphaeria pullmanensis. The remaining 26 isolates were identified as Cytospora chrysosperma. Pathogenicity of Cr. pullmanensis on two‐year‐old P. nigra and Populus alba saplings under glasshouse conditions confirmed that Cr. pullmanensis is pathogenic on P. nigra and P. alba. Cryptosphaeria pullmanensis is here reported from Iran causing Cryptosphaeria canker on poplar trees for the first time. However, its host range, the extent of geographical distribution and management strategies remain to be examined.

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“…and cause similar disease symptoms in these hosts (Hinds ; Vasilyeva and Ma ; Trouillas et al. ). However, Cr.…”

Section: Discussionmentioning

confidence: 85%

“…pullmanensis (Trouillas et al. ). Cryptosphaeria subcutanea is morphologically the closest taxon to Cr.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cryptosphaeria Canker of Populus nigra Caused by Cryptosphaeria pullmanensis, a New Threat to Poplar Industry in Iran

Aghdam

Babai-Ahari

Karimi

et al. 2017

Journal of Phytopathology

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“…A taxonomic review of the genus in the western United States was recently conducted by Trouillas, Hand, Inderbitzin, and Gubler (2015). According to the Dictionary of Fungi, the genus Cryptosphaeria is a member of Diatrypaceae, which consists of eight main genera.…”

Section: Introductionmentioning

confidence: 99%

First report of Cryptosphaeria pullmanensis as causal agent of Cryptosphaeria canker of Populus nigra in Iran

2017

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A diatrypaceous fungus was isolated consistently from cankers on Populus nigra trees showing dieback symptoms in Kohgiluyeh Boyer-Ahmad, Zanjan and Esfahan provinces in Iran. Morphological characteristics and phylogenetic analyses of the ITS region of the rDNA identified the taxon as Cryptosphaeria pullmanensis. Pathogenicity tests conducted in potted 3-month-old cuttings of P. nigra confirmed Koch's postulates and revealed that C. pullmanensis caused canker on this host. This is the first report of C. pullmanensis causing a canker disease on P. nigra in Iran.2 weeks in the dark at 24°C, covering a 60-mm Petri dish after 8 days ( Figure 1i). Teleomorph (sexual stage) and anamorph (asexual stage) morphologies of the isolates from Iran were in close agreement with morphological descriptions of Glawe (1984) and Trouillas et al. (2010). The anamorph of the fungus also resembles the form genus CytosporaEhrenb. with respect to the multichambered conidiomata and allantoid conidia (Glawe, 1984). However, C. pullmanensis can be distinguished from species of Cytospora by having annellides, sympodulate, longer and larger conidia (Glawe, 1984;. A total of 20 strains associated with Cryptosphaeria canker were isolated from P. nigra trees growing in three provinces of Iran. Single isolates of both types of morphs yielded identical colonies, showing that both types of morphs belonged to the same fungus. Three isolates (one from each district surveyed) isolated from cankers and two additional strains (IRAN 2426C and IRAN 2512C) isolated from conidiomata and ascomata on the host tissue were sequenced. Five new ITS sequences were obtained and aligned with 21 reference sequences retrieved from GenBank (Table 1). The length of our sequences was between 530 and 540 bp and was identical among the five isolates. Based on a BLASTn search of NCBI GenBank nucleotide database, the closest sequence to our fungus was C. pullmanensis (GenBank KT428044; identities = 530/531(99%), gaps = 1/531(0%)). Isolates obtained in this study grouped in a well-supported clade (bootstrap value = 100%) with C. pullmanensis (Figure 2). Based on both morphology and ITS sequencing, the occurrence of this pathogen in Iran was confirmed.In the pathogenicity test, cankers with an average size of 1-3 cm in length, 1-2 cm wide and 0.3-0.6 mm depth formed after 5 months and were similar to those occurring on naturally infected stems.Following removal of the bark, the necrotic phloem was exposed and a dark brown discoloration of the wood was visible (Figure 1f-h).The fungus produced asexual fruiting bodies and spore tendrils around the inoculation site after 5 months that were similar to those occurring on infected hosts described above (Figure 1f). The pathogen was re-isolated consistently from the lesions, which confirmed Koch's postulates. Percent fungal recovery for all of three isolates tested was 100%, whereas no fungi were isolated from the control plants. Pathogenicity tests using three isolates of different origin showed no differences in virulence or pathoge...

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Fungal pathogens associated with branch and trunk cankers of nut crops in Iran

et al. 2020

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Branch and trunk canker diseases have become prevalent on nut crops in Iran. During 2015 to 2018, extensive field surveys were conducted on 58 almond, 43 pistachio and 80 walnut orchards in Iran to study fungal pathogens associated with symptomatic trees. One hundred and fifty-six representative fungal isolates were selected and identified based on morphological characteristics and by phylogenetic comparison of DNA sequence data. Fungal species found were Collophorina hispanica, Pleurostoma richardsiae, nine species of Phaeoacremonium (namely P. angustius, P.

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The genus Cryptosphaeria in the western United States: taxonomy, multilocus phylogeny and a new species, C. multicontinentalis

Cited by 26 publications

References 3 publications

Cryptosphaeria Canker of Populus nigra Caused by Cryptosphaeria pullmanensis, a New Threat to Poplar Industry in Iran

Cryptosphaeria Canker of Populus nigra Caused by Cryptosphaeria pullmanensis, a New Threat to Poplar Industry in Iran

First report of Cryptosphaeria pullmanensis as causal agent of Cryptosphaeria canker of Populus nigra in Iran

Fungal pathogens associated with branch and trunk cankers of nut crops in Iran

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