The genotoxicity of iron and chromium in electroplating effluents

Godet, F. Arnaud; Babut, Marc; Burnel, D; Veber, Anne-Marie; Vasseur, Paule

doi:10.1016/s0165-1218(96)90123-8

Cited by 43 publications

(20 citation statements)

References 9 publications

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“…Hexavalent Cr was reported to induce chromosomal aberrations, micronuclei, and singlestrand DNA breaks in mammalian cells [Wise et al, 2002], and gene mutation in bacteria [De Flora et al, 1990]. Trivalent Fe also was reported to induce a high level of micronuclei in newt larvae at concentrations of 12.5 and 25 mg/l [Godet et al, 1996]. Exposure of mice to Zn results in single-strand breaks in DNA as measured Environmental and Molecular Mutagenesis.…”

Section: Discussionmentioning

confidence: 99%

“…The combined effects of Fe and Cr produced an elevated percentage of micronucleated red blood cells and other genotoxic effects in assays conducted with newt larvae [Godet et al, 1996]. Cu produces free radicals, and when present in an unbound form, it produces reactive oxygen species that cause DNA, protein, and lipid damage [Galaris and Evangelou, 2002].…”

Section: Discussionmentioning

confidence: 99%

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DNA damage induced in human peripheral blood lymphocytes by industrial solid waste and municipal sludge leachates

Bakare

Pandey

Bajpayee

et al. 2006

Environ and Mol Mutagen

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Exposure of humans to toxic compounds occurs mostly in the form of complex mixtures. Leachates, consisting of mixtures of many chemicals, are a potential risk to human health. In the present study, leachates of solid wastes from a polyfiber factory (PFL), an aeronautical plant (AEL), and a municipal sludge leachate (MSL) were assessed for their ability to induce DNA damage in human peripheral blood lymphocytes using the alkaline Comet assay. The leachates also were examined for their physical and chemical properties. Lymphocytes were incubated with 0.5-15.0% concentrations (pH range 7.1-7.4) of the test leachates for 3 hr at 37 degrees C, and treatment with 1 mM ethyl methanesulfonate served as a positive control. All three leachates induced significant (P < 0.05), concentration-dependent increases in DNA damage compared with the negative control, as measured by increases in Olive tail moment (arbitrary units), tail DNA (%), and tail length (mum). A comparison of these variables among the treatment groups indicated that the MSL induced the most DNA damage. Inductively coupled plasma emission spectrometry analysis of the leachates indicated that they contained high concentrations of heavy metals, viz. iron, manganese, nickel, zinc, cadmium, chromium, and lead. The individual, synergistic, or antagonistic effects of these chemicals in the leachates may be responsible for the DNA damage. Our data indicate that the ever-increasing amounts of leachates from waste landfill sites have the potential to induce DNA damage and suggest that the exposure of human populations to these leachates may lead to adverse health effects.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

DNA damage induced in human peripheral blood lymphocytes by industrial solid waste and municipal sludge leachates

Bakare

Pandey

Bajpayee

et al. 2006

Environ and Mol Mutagen

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“…Vagrants arise as a result of irregular separation and dislocation of chromosomes: thereby constituting a risk of aneuploidy (Maluszynska and Juchimiuk, 2005). C-mitosis is one of the consequences of inactivation of spindle apparatus connected with delay in the division of centromere (Mann, 1977) Trace elements and other chemical pollutants acting singly or in their combined states have been implicated in induction of genetic abnormalities in biological systems (Godet et al, 1993;Chauhan et al, 1999;Kong and Ma, 1999;Babatunde and Bakare, 2006). Lead has been known to cause reduction in root growth and frequency of mitotic cells in the meristematic zone of onions.…”

Section: Discussionmentioning

confidence: 99%

Toxicity Evaluation and Cytogenetic Screening of Process Water Using a Plant Bioassay

Olorunfemi¹,

Olomukoro²,

Anani³

2015

Nig J Bas App Sci

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ABSTRACT:The effect of toxic substances on aquatic lives from a wastewater indiscriminately discharged into the environment during oil and gas exploration activities in Nigeria is the focus of this study. A plant bioassay, the Allium cepa test, was used for the cytogenotoxicity screening of process water on root growth inhibition and chromosome abnormalities in the meristematic roots of the plant. Results of the physicochemical analysis of the wastewater collected from Nigerian Agip Oil Company (NAOC) facility at Ogboinbiri in Bayelsa State at the point of discharge into the environment showed that it had an unpleasant odour, was slightly alkaline (pH 7.72) and had high electrical conductivity (2793.33 µS/cm). Lead, manganese, iron, chromium and nickel were present at amounts (0.13, 0.33, 4.47, 0.17 and 0.10 mg/l respectively) above national (NESREA) and international (USEPA) limits for effluent discharge. Macroscopic evaluation of A. cepa cultivated in the wastewater resulted in significant (p<0.05) concentration-dependent root growth inhibition with an EC 50 value of 14.8 % at 96 h. Root tip cells of the onion bulbs processed for cytological studies by the aceto-orcein squash technique after exposure to the wastewater for 48 h at concentrations of 0.5, 1.0, 2. 5, 5.0, and 10% (v/v; wastewater/tap water) showed chromosomal aberrations at all concentrations. Statistical analysis of microscopic results show significant (p<0.05) concentration-dependent frequency of aberrant chromosomes and reduction of mitotic index. The findings in this study calls for proper treatment of process water before its discharge into water bodies to avoid cyto-genetic damages to aquatic lives.

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“…O potencial de toxicidade destas misturas complexas ainda esta sobre discussão na literatura cientifica, porém, a maioria dos estudos foram realizados principalmente em bactérias (Claxton et al, 1998;Siddiqui e Ahmad, 2003), e outros organismos como leveduras (Schmitt et al, 2005), plantas (Smaka-Kincl et al, 1996;Grover e Kaur, 1999), anfíbios (Godet et al, 1996;Zoll-Moreux e Ferrier, 1999), células de mamíferos (Reifferscheid et al, 2008) e peixes (Dizer et al, 2002). O efeito mutagênico e carcinogênico de substâncias individuais que podem estar presentes nestas misturas são bem conhecidos (Cabrera et al, 1999).…”

Section: Introductionunclassified

Indução de micronúcleos e toxicidade por efluente doméstico em duas populações de Bathygobius soporator (Valenciennes, 1837) (Teleostei, Gobbidae) no litoral de Salvador (BA), Brasil

Galindo¹,

Silva²,

Rosário³

2012

Braz. J. Aquat. Sci. Technol.

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Fish micronuclei tests were used to evaluate the potential of municipal wastewater sewage to cause genetic change in Bathygobius soporator. The levels of toxicity of municipal wastewater sewage were evaluated in populations of B. soporator originating from metal-contaminated and metal-free areas. Blood samples were taken from ten fish caught in sites with low anthropogenic influence to measure the levels of spontaneous micronucleated erythrocytes. An additional 30 fish specimens were captured to conduct laboratory testing. After a 30-day acclimation period, blood samples were collected under controlled conditions, while fish that were not sampled from blood were exposed to concentration of 10 % of the urban effluents for 10 days. Acute toxicity experiments were also carried out at different concentrations (0, 1, 3, 7, 10, and 30%) of urban effluent for 48-h in using fish specimens of the two sampled populations of B. soporator. The results of this study showed that mortality was threefold higher for fish exposed to the urban effluents in the population of metal-free area compared with fish from the metal-contaminated area. There were micronucleus inductions in fish after 10 days of exposures to the urban effluents and significant differences (p< 0.01) after only 10 days when comparing to spontaneous micronucleus levels. We concluded that were microncleus inductions from urban effluents leading to higher mortality of individuals from the population of metal-free area, and this was higher than for individuals of the population exposed to metal contaminants.

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The genotoxicity of iron and chromium in electroplating effluents

Cited by 43 publications

References 9 publications

DNA damage induced in human peripheral blood lymphocytes by industrial solid waste and municipal sludge leachates

DNA damage induced in human peripheral blood lymphocytes by industrial solid waste and municipal sludge leachates

Toxicity Evaluation and Cytogenetic Screening of Process Water Using a Plant Bioassay

Indução de micronúcleos e toxicidade por efluente doméstico em duas populações de Bathygobius soporator (Valenciennes, 1837) (Teleostei, Gobbidae) no litoral de Salvador (BA), Brasil

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