Indução de micronúcleos e toxicidade por efluente doméstico em duas populações de Bathygobius soporator (Valenciennes, 1837) (Teleostei, Gobbidae) no litoral de Salvador (BA), Brasil

Abstract

Fish micronuclei tests were used to evaluate the potential of municipal wastewater sewage to cause genetic change in Bathygobius soporator. The levels of toxicity of municipal wastewater sewage were evaluated in populations of B. soporator originating from metal-contaminated and metal-free areas. Blood samples were taken from ten fish caught in sites with low anthropogenic influence to measure the levels of spontaneous micronucleated erythrocytes. An additional 30 fish specimens were captured to conduct laboratory testing. After a 30-day acclimation period, blood samples were collected under controlled conditions, while fish that were not sampled from blood were exposed to concentration of 10 % of the urban effluents for 10 days. Acute toxicity experiments were also carried out at different concentrations (0, 1, 3, 7, 10, and 30%) of urban effluent for 48-h in using fish specimens of the two sampled populations of B. soporator. The results of this study showed that mortality was threefold higher for fish exposed to the urban effluents in the population of metal-free area compared with fish from the metal-contaminated area. There were micronucleus inductions in fish after 10 days of exposures to the urban effluents and significant differences (p< 0.01) after only 10 days when comparing to spontaneous micronucleus levels. We concluded that were microncleus inductions from urban effluents leading to higher mortality of individuals from the population of metal-free area, and this was higher than for individuals of the population exposed to metal contaminants.

Análise Do Polimorfismo De Deleção Dos Genes Gstm1 E Gstt1 E Diabetes Mellitus Em Idosos: Estudo Piloto

“…Os resultados obtidos para o Bacanca estão em consonância com os de outros estudos desenvolvidos no local. Martins (2008) A frequência de micronúcleos e deformidades nucleares pode ser considerada uma resposta complexa entre a atividade genotóxica e a eficiência do mecanismo fisiológico de defesa do organismo teste (MERSCH, BEAUVAIS, 1997;GALINDO et al, 2012), uma vez que as células sofrem alterações na distribuição ou quebra de suas cromátides durante a anáfase, onde os fragmentos cromossômicos não acompanham a migração para os pólos da célula, e, após a telófase, tais fragmentos não são incluídos nos núcleos das células filhas, formando um único ou múltiplos micronúcleos no citoplasma dessas células (VON LEDEBUR, SCHMID, 1973), ou anomalias no contorno da carioteca, devido a ação de agentes tóxicos (FERREIRA, 2004).…”

A review of ancestrality and admixture in Latin America and the caribbean focusing on native American and African descendant populations

“…However, no more than 30 communities have been analyzed regarding their genomic background ( Kimura et al, 2017 ). In general, Quilombola communities are deprived of treated water sources, garbage collection, and sewage ( Galindo et al, 2012 ), which could expose the residents to harmful substances and mutagenic agents. A genotoxicity and mutagenicity analysis revealed evidence of genomic damage in Quilombola communities in the State of Goiás, Brazil ( de Moraes Filho et al, 2020 ).…”

Evaluation of aquatic contamination in an urban lagoon environment in a coastal region in the north of Brazil

“…The micronucleus test for the biomonitoring of aquatic environments has been used by several authors in different species due to its practicality and effectiveness. Bombail et al, (2001) applied the test in Pholis gunnellus along a pollution gradient in the Firth of Forth, Scotland, UK; Galindo and Moreira (2009) used it on species of tropical marine fish (Bathygobius soporator); and Galindo et al, (2012) evaluated the potential of sewage to cause genetic alterations in blood cells in Bathygobius soporator populations from the coast of Salvador, Bahia, Brazil.…”