The genomic sequence of cowpea aphid-borne mosaic virus and its similarities with other potyviruses

Mlotshwa, Sizolwenkosi; Verver, J.; Sithole-Niang, Idah; Kampen, Tineke; Kammen, A. van; Wellink, J.

doi:10.1007/s00705-002-0800-z

Cited by 17 publications

(5 citation statements)

References 35 publications

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“…Complete CP coding sequence containing 828 nucleotides and 275 amino acids were identified in all ten CABMV-Lns isolates, in agreement with the sequenced CABMV genomes (Mlotshwa et al, 2002;Barros et al, 2011). Very little variation was found amongst the nucleotide sequence of CABMV-Lns isolates (identity from 97 to 99%) ( Table 1).…”

Section: Analysis Of Coat Protein Sequencesupporting

confidence: 77%

Recent characterization of cowpea aphid-borne mosaic virus (CABMV) in Bahia State, Brazil, suggests potential regional isolation

Melo¹,

Figueira²,

Moreira³

et al. 2015

Afr. J. Biotechnol.

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Woodiness disease is the most important disorder of passion fruit worldwide. The causal agent in Brazil is the Cowpea aphid-borne mosaic virus (CABMV), and despite the economic relevance of passion fruit for agriculture there have been recently very few studies about this virus in Brazil and worldwide. This work reveals the phylogenetic relationships of 10 newly identified CABMV isolates from Bahia State, the region where CABMV was first identified (in that time reported as PWV) in South America before its outbreak. The coat protein of 10 CABMV isolates (CABMV-Lns1-CABMV-Lns10) from Livramento de Nossa Senhora Country, Bahia State, were sequenced and presented very close identity between themselves (nucleotide: 97 to 99%, amino acid: 95 to 100%). They are phylogenetically closely related to Brazilian CABMV, however forming an isolated cluster within the Brazilian clade. According to previous evidences, our data demonstrate that CABMV-Lns are more closely related to isolates from Southern rather than from Northern Africa. Other two isolates from Bahia State clustered separately from CABMV-Lns, but together with isolates from other Brazilian regions thus suggesting that CABMV-Lns are a strain likely restricted to Bahia. The characterization of new populations of CABMV enables greater resolution of the evolution of viruses causing woodiness disease in passion fruit vines. Our data shed light on an as yet unexplored population of CABMV in Brazil and contributes to the understanding of its evolutionary history.

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Section: Analysis Of Coat Protein Sequencesupporting

confidence: 77%

Recent characterization of cowpea aphid-borne mosaic virus (CABMV) in Bahia State, Brazil, suggests potential regional isolation

Melo¹,

Figueira²,

Moreira³

et al. 2015

Afr. J. Biotechnol.

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“…The first inframe translation start codon AUG (130–132) of the three isolates genomic RNA was situated in the appropriate context of AGCAAUGGC (CCLB and LWLB isolates) and AGAAAUGGC (WFLB14 isolate), which were similar to the corresponding sequence AACAAUGGC in plants [ 22 ]. The −3 and +4 nucleotides were both purines that might be required for initiation of translation [ 23 ].…”

Section: Resultsmentioning

confidence: 99%

Molecular Characterization of the Complete Genome of Three Basal-BR Isolates of Turnip mosaic virus Infecting Raphanus sativus in China

Zhu

Sun

Wang

et al. 2016

IJMS

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Turnip mosaic virus (TuMV) infects crops of plant species in the family Brassicaceae worldwide. TuMV isolates were clustered to five lineages corresponding to basal-B, basal-BR, Asian-BR, world-B and OMs. Here, we determined the complete genome sequences of three TuMV basal-BR isolates infecting radish from Shandong and Jilin Provinces in China. Their genomes were all composed of 9833 nucleotides, excluding the 3′-terminal poly(A) tail. They contained two open reading frames (ORFs), with the large one encoding a polyprotein of 3164 amino acids and the small overlapping ORF encoding a PIPO protein of 61 amino acids, which contained the typically conserved motifs found in members of the genus Potyvirus. In pairwise comparison with 30 other TuMV genome sequences, these three isolates shared their highest identities with isolates from Eurasian countries (Germany, Italy, Turkey and China). Recombination analysis showed that the three isolates in this study had no “clear” recombination. The analyses of conserved amino acids changed between groups showed that the codons in the TuMV out group (OGp) and OMs group were the same at three codon sites (852, 1006, 1548), and the other TuMV groups (basal-B, basal-BR, Asian-BR, world-B) were different. This pattern suggests that the codon in the OMs progenitor did not change but that in the other TuMV groups the progenitor sequence did change at divergence. Genetic diversity analyses indicate that the PIPO gene was under the highest selection pressure and the selection pressure on P3N-PIPO and P3 was almost the same. It suggests that most of the selection pressure on P3 was probably imposed through P3N-PIPO.

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“…Some authors underlined the necessity to use transgenic resistant varieties, due to the limited number of natural resistance sources and problems of stability of this resistance (Hampton and Thottappilly, 2003). This approach could take advantage of possibilities given by the determination of the complete sequence of the CABMV genome (Mlotshwa et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Yield Losses Caused by Cowpea Aphid-borne mosaic virus (CABMV) in 21 Cowpea (Vigna unguiculata (L.) Walp.) Varieties in Burkina Faso

Néya¹,

Zida²,

Sérémé³

et al. 2015

Pakistan J. of Biological Sciences

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02/08 Fax : (226) 25 31 92 75 A B S T R A C T Cowpea (Vigna unguiculata (L.) Walp.) is one the main leguminous crop plants world wide, particularly in sub-Saharan Africa where the rainfall is low and often unevenly distributed. However, its production is limited by insect attacks and numerous diseases including those caused by viruses. In Burkina Faso, the Cowpea Aphid-Borne Mosaic Virus (CABMV) is one of the viruses capable of causing 7-60% of yield losses. The aim of this study is to assess the susceptibility of 21 cowpea varieties against CABMV and to also assess the yield losses caused by this virus on each of the varieties. In this context trial was conducted on INERA research stations at Kamboinsé and Farako-Bâ in 2012 and 2013. Twenty one varieties were used for this study. The experimental design used is a split-plot with 4 repetitions for which the first 2 repetitions were inoculated with the CABMV. Symptoms caused by the CABMV on the 21 varieties of cowpea were of a great diversity. The assessment of the virus in infected plant samples from these varieties by ELISA test allowed detecting the mosaic virus in all samples that presented mosaic symptoms. The number of flowers and pods strongly varied depending to the type of plant (inoculated or non-inoculated plant) and the variety. Yields from the different varieties, comprised between 187 and 6250 kg haG 1 , were influenced by the site, the year, the variety and the type of plant. Yields losses were ranged from 3-64% depending to the variety.

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The genomic sequence of cowpea aphid-borne mosaic virus and its similarities with other potyviruses

Cited by 17 publications

References 35 publications

Recent characterization of cowpea aphid-borne mosaic virus (CABMV) in Bahia State, Brazil, suggests potential regional isolation

Recent characterization of cowpea aphid-borne mosaic virus (CABMV) in Bahia State, Brazil, suggests potential regional isolation

Molecular Characterization of the Complete Genome of Three Basal-BR Isolates of Turnip mosaic virus Infecting Raphanus sativus in China

Evaluation of Yield Losses Caused by Cowpea Aphid-borne mosaic virus (CABMV) in 21 Cowpea (Vigna unguiculata (L.) Walp.) Varieties in Burkina Faso

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