The Genome Sequence of Avian Pathogenic
            <i>Escherichia coli</i>
            Strain O1:K1:H7 Shares Strong Similarities with Human Extraintestinal Pathogenic
            <i>E. coli</i>
            Genomes

Johnson, Timothy J.; Kariyawasam, Subhashinie; Wannemuehler, Yvonne; Mangiamele, Paul; Johnson, Sara J.; Doetkott, Curt; Skyberg, Jerod A.; Lynne, Aaron M.; Johnson, James R.; Nolan, Lisa K.

doi:10.1128/jb.00537-07

Cited by 114 publications

(178 citation statements)

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“…Some of the genes that were found to be widely distributed among both UPEC and APEC have been localized to large transmissible plasmids and pathogenicity islands (PAIs). This similarity supports the hypothesis that birds may act as a reservoir for potentially zoonotic E. coli strains (Ewers et al 2007, Johnson et al 2007.…”

Section: Introductionsupporting

confidence: 75%

“…The epidemiological link between human and animal disease are well established in some instances but remain unclear in others (Johnson et al 2007). Recent isolation reports of diarrheagenic Escherichia coli in wild birds reinforce the idea of bacterial transmission from humans to birds (Saidenberg 2008, Knöbl & Menão 2010 (Vidal et al 2005).…”

Section: Introductionmentioning

confidence: 99%

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Serogroups and virulence genes of Escherichia coli isolated from psittacine birds

et al. 2011

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916Pesq. Vet. Bras. 31(10):916-921, outubro 2011 RESUMO.-[Sorogrupos e genes de virulência em Escherichia coli isoladas de psitacídeos.] Amostras de Escherichia coli isoladas de 24 psitacídeos doentes foram sorogrupadas e investigadas para a presença de genes que codiϐicam os seguintes fatores de virulência: attaching e effacing (eae), plasmídeo EAF (EAF), pili associado à pielonefrite (pap), ϐímbria S (sfa), adesina aϐimbrial (afa), cápsula K1 (neu), curli (crl, csgA), hemaglutinina termosensível (tsh), enterotoxina termo-estável 1 de E. coli enteroagregativa (astA), toxina termolábil (LT) e toxina termoestável (STa e STb), Shiga-like toxinas (stx1 e stx2), fator citotóxico necrotizante 1 (cnf1), hemolisina (hly), produção de aerobactina (iuc) e resistência sérica (iss). Os resultados mostraram que os isolados pertenciam a 12 sorogrupos: O7; O15; O21; O23; O54; O64; O76; O84; O88; O128; O152 e O166. Os genes de virulência encontrados foram: crl em todos os isolados, pap em 10 isolados, iss em sete isolados, csgA em cinco isolados, iuc e tsh em três isolados e eae em dois isolados. A combinação dos genes de virulência revelou 11 perϐis genotípicos distintos. Todas as amostras foram negativas para os genes que codiϐicam EAF, EAEC, K1, sfa, afa, hly, cnf, LT, STa, STb, stx1 e stx2. Estes resultados demonstraram que algumas amostras de E. coli isoladas de psitacídeos apresentam os mesmos fatores de virulência presentes nos patotipos de E. coli patogênicas para aves (APEC), uropatogênicas (UPEC) e E. coli enteropatogênicas (EPEC). Escherichia coli isolates from 24 sick psittacine birds were serogrouped and investigated for the presence of genes encoding the following virulence factors: attaching and effacing (eae), enteropathogenic E. coli EAF plasmid (EAF), pili associated with pyelonephritis (pap), S ϐimbriae (sfa), aϐimbrial adhesin (afa), capsule K1 (neu), curli (crl, csgA), temperature--sensitive hemagglutinin (tsh), enteroaggregative heat-stable enterotoxin-1 (astA), heat--stable enterotoxin -1 heat labile (LT) and heat stable (STa and STb) enterotoxins, Shiga-like toxins (stx1 and stx2), cytotoxic necrotizing factor 1 (cnf1), haemolysin (hly), aerobactin production (iuc) and serum resistance (iss). The results showed that the isolates belonged to 12 serogroups: O7; O15; O21; O23; O54; O64; O76; O84; O88; O128; O152 and O166. The virulence genes found were: crl in all isolates, pap in 10 isolates, iss in seven isolates, csgA in ϐive isolates, iuc and tsh in three isolates and eae in two isolates. The combination of virulence genes revealed 11 different genotypic patterns. All strains were negative for genes encoding for EAF, EAEC, K1, sfa, afa, hly, cnf, LT, STa, STb, stx1 and stx2. Our ϐindings showed that some E. coli isolated from psittacine birds present the same virulence factors as avian pathogenic E. coli (APEC), uropathogenic E. coli (UPEC) and Enteropathogenic E. coli (EPEC) pathotypes. Serogroups and virulence genes of

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Section: Introductionsupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Serogroups and virulence genes of Escherichia coli isolated from psittacine birds

et al. 2011

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“…The phylogenetic relationships found among ExPEC (Extraintestinal pathogenic E. coli) APEC, UPEC (Uropathogenic E. coli) and NMEC (Newborn meningitis-causing E. coli) strains highlight the zoonotic potential of strains of avian origin (JOHNSON et al, 2007). Thus, the study of antimicrobial susceptibility of these isolates becomes an important health public issue.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial susceptibility and pathogenicity of Escherichia coli strains of environmental origin

et al. 2015

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The study aimed to evaluate the antimicrobial susceptibility of 109 samples of Escherichia coli (E. coli) of environmental origin and to characterize these isolates according to the degree of pathogenicity in vivo, verifying a possible relationship between this variable and susceptibility to the active principles tested. The isolates were subjected to disc diffusion test to 14 antibiotics. From 16.5% to 90% of the samples were sensitive; 1 - 28.5% showed intermediate degree of susceptibility and between 9 to 78% of E. coli analyzed were resistant. The highest resistance percentages were seen in the class of quinolones and tetracyclines (>75%), and for sensitivity in the class of amphenicols (68.8%). By inoculating 1- day - old chicks, the isolates were classified as highly pathogenic (2.7%), intermediate (10.1%), low (42.2%) and apathogenic (45%). It was observed a wide variation in the susceptibility profile of isolates in relation to antimicrobials. It was also found that most of the samples had pathogenic potential (55%), thus being considered as APEC (avian pathogenic E. coli). No relationship between pathogenicity and antimicrobial susceptibility (P≤0.05) was observed.

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“…A considerable amount of information on avian colibacillosis now exists and the molecular virulence determinants of APEC are slowly being unravelled. The recent availability of an APEC O1:K1:H7 complete genome sequence (Johnson et al, 2007a) provides a turning point in our understanding of APEC and also an appropriate launching pad for the application of genetic methods to define the APEC pathotype and virulence. Novel approaches to survey the repertoire, sequence, expression and role in virulence of APEC genes in controlled infection models provide an excellent opportunity to improve our knowledge on APEC virulence mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Colibacillosis in poultry: unravelling the molecular basis of virulence of avian pathogenicEscherichia coliin their natural hosts

Dziva¹,

Stevens²

2008

Avian Pathology

283

210

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Avian colibacillosis is caused by a group of pathogens designated avian pathogenic Escherichia coli (APEC). Despite being known for over a century, avian colibacillosis remains one of the major endemic diseases afflicting the poultry industry worldwide. Autologous bacterins provide limited serotype-specific protection, yet multiple serogroups are associated with disease, especially O1, O2 and O78 among many others. Experimental infection models have facilitated the identification of some key APEC virulence genes and have allowed testing of vaccine candidates. Well-recognized virulence factors include Type 1 (F1) and P (Pap/ Prs) fimbriae for colonization, IbeA for invasion, iron acquisition systems, TraT and Iss for serum survival, K and O antigens for anti-phagocytic activity, and a temperature-sensitive haemagglutinin of imprecise function. Intriguingly, these factors do not occur universally among APEC, suggesting the presence of multiple alternative mechanisms mediating pathogenicity. The recent availability of the first complete APEC genome sequence can be expected to accelerate the identification of bacterial genes expressed during infection and required for virulence. High-throughput molecular approaches like signature-tagged transposon mutagenesis have already proved invaluable in revealing portfolios of genes expressed by pathogenic bacteria during infection, and this has enabled identification of APEC O2 factors required for septicaemia in the chicken model. Complimentary approaches, such as in vivo-induced antigen technology, exist to define the activities of APEC in vivo. In recent years, reverse vaccinology and immuno-proteomic approaches have also enabled identification of novel vaccine candidates in other bacterial pathogens. Collectively, such information provides the basis for the development or improvement of strategies to control APEC infections in the food-producing avian species.

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The Genome Sequence of Avian Pathogenic Escherichia coli Strain O1:K1:H7 Shares Strong Similarities with Human Extraintestinal Pathogenic E. coli Genomes

Cited by 114 publications

References 0 publications

Serogroups and virulence genes of Escherichia coli isolated from psittacine birds

Serogroups and virulence genes of Escherichia coli isolated from psittacine birds

Antimicrobial susceptibility and pathogenicity of Escherichia coli strains of environmental origin

Colibacillosis in poultry: unravelling the molecular basis of virulence of avian pathogenicEscherichia coliin their natural hosts

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