The Genome Organization of Potato Virus M RNA

Abstract

The 8534 nucleotide sequence of the genome of the carlavirus, potato virus M (PVM), has been determined. The sequence contains six large open reading frames (ORFs) and non-coding regions consisting of 75 nucleotides at the 5' end, 70 nucleotides followed by a poly(A) tail at the 3' end and 38 and 21 nucleotides between three large blocks of coding sequences. The ORF beginning at the first initiation codon at nucleotide 76 encodes a polypeptide of 223K which, according to its primary sequence analysis, seems to be a virus RNA replicase. The next coding block consists of three ORFs encoding polypeptides of 25K, 12K and 7K. The third block consists of two ORFs encoding polypeptides of 34K (PVM coat protein) and llK. The llK polypeptide contains a pattern resembling the consensus for a metal-binding nucleic acid-binding 'finger'. The nucleotide sequence EMBL accession number is X53062.

Nucleotide sequence and gene organization of the 3'-terminal region of chrysanthemum virus B genomic RNA

“…They consist of multiple copies of a coat protein (Mr 37000) encapsidating the viral RNA (Hollings & Stone, 1972). Recently, there have been reports of the complete nucleotide sequence of the genomic RNA of carlavirus potato virus M (PVM) (Zavriev et al, 1991) and the 3'-terminal regions of other carlavirus genomic RNAs such as those of potato virus S (PVS), lily symptomless virus (LSV), helenium virus S (HelVS) and carnation latent virus (CLV) (MacKenzie et al, 1989;Memelink et al, 1990;Foster et al, 1990;Haylor et al, 1990). These carlaviruses have an identical genome organization, which is similar to that of potexviruses.…”

“…4) upstream of the AUG codons of the 25K protein and the coat protein genes. In all carlavirus RNA sequences known to date (MacKenzie et al, 1989;Memelink et al, 1990;Foster et al, 1990;Zavriev et al, 1991), this consensus sequence is found in only the two regions indicated above and may be essential for the initiation of carlavirus subgenomic RNA synthesis.…”

Intermolecular Interactions of Chrysanthemum virus B Coat Protein: Implications for Capsid Assembly

“…CVB has slightly flexuous rod shaped particle of 680 9 12 nm and is transmitted by aphid Myzus persiceae in a non-persistent manner and is also sap transmissible. Carlavirus genome comprises of single stranded monopartite, plus sense ssRNA molecule *8.8 kb in size with a poly (A) tail at 3 0 -terminus and a cap structure or a monophosphate at their 5 0 -terminus [4,5,15,31]. The genomic RNA is encapsidated by a single type of coat protein (CP) with molecular weight 34 kDa.…”

Inference on Coat Protein Evolution of Lily Symptomless Carlavirus in India and Abroad Based on Motifs Study and Phylogenetic Analysis

“…CP subunits are of one type, and 31-36 KDa in size (Adams et al, 2004). The carlaviral genomes have a poly (A) tract at their 3´-terminus and a cap structure or a monophosphate at their 5´-terminus (Zavriev et al, 1991). The genus comprises of more than 50 viruses such as Chrysanthemum virus B (CVB), Potato virus M (PVM), Hop latent virus (HpLV), Lily symptomless virus (LSV), Daphne virus S (DVS), Helenium virus S (HelVS), Garlic common latent virus (GarCLV), etc.…”

“…The genome is a single stranded RNA 7.4-8.5 Kb in size (Cavileer et al, 1994;Fugi et al, 2002;Zavriev et al, 1991) and comprises six ORFs, encoding, in order, the replication related proteins, the putative movement proteins (MP) i.e. triple gene block (TGB), the coat protein (CP) and a putative nucleic acid binding regulatory protein (NABP).…”