Intermolecular Interactions of Chrysanthemum virus B Coat Protein: Implications for Capsid Assembly

Abstract

Chrysanthemum virus B coat protein constitutes the viral capsid which, besides other functions, encapsulates and protects the viral nucleic acid. We have demonstrated homotypic interaction of the coat protein subunits, essentially important for dimer formation, which is the first step during capsid assembly in vivo and in vitro. Interaction capacity of full length and truncated protein has been investigated and important regions have been identified through protein-protein interaction in yeast and by coimmunoprecipitation assays. Complete coat protein was found to interact strongly with similar subunits. Constructs with 102 amino acids from the N-terminal and 64 amino acids from C-terminal were found to be inconsequential for dimer formation as they did not show any interaction with similar subunits or with full length protein when analysed for b-galactosidase or histidine prototrophy. Results suggest that the region of 98-184 amino acids from the middle plays an important role in the process, probably without the involvement of N-and C-terminals.

Current Achievements and Future Prospects in Virus Elimination Technology for Functional Chrysanthemum

“…Nine types of chrysanthemum viruses [19,28,44], namely, chrysanthemum virus B (CVB) [44,45], cucumber mosaic virus (CMV) [25], tomato mosaic virus (TMV) [25], tomato aspermy virus (TAV) [46], potato virus X (PVX) [46], potato virus Y (PVY) [47], tomato spotted wilt virus (TSWV) [48], chrysanthemum stunt viroid (CSVd) [49][50][51] and chrysanthemum chloritic mottle viroid (CChMVd) [52,53], have been reported in major functional chrysanthemum-producing areas. Most chrysanthemum viral diseases are quarantine hazards and can spread to a large area.…”

A multiplex RT-PCR for rapid and simultaneous detection of viruses and viroids in chrysanthemum

“…; Singh et al . ), tomato aspermy virus (TAV) (Raj et al . ), cucumber mosaic virus (CMV) (Kumar et al .…”