The Genetic Variability of Prolactin and Signal Transducers and Activators of Transcription 5A (STAT5A) Genes in Bali Cattle

Abstract

The aim of this study was to identify the genetic variability of PRL and STAT5A genes in Bali cattle from Bali, West Nusa Tenggara (NTB), and South Sulawesi (SS) using PCR-RFLP method. A total of 262 Bali cattle were identified using RsaI (PRL) and AvaI (STAT5A) restriction enzymes. PRL gene exon 3, PRL gene exon 4, and STAT5A gene exon 7 amplifications resulted fragments with the lengths of 156 bp, 294 bp, 215 bp, respectively. Genotyping of PRL gene both at exon 3 and 4 produced three genotypes in Bali population and two genotypes in NTB and SS population. For the PRL gene, frequencies of A allele (exon 3) and G allele (exon 4) were dominant to the B allele (exon 3) and A allele (exon 4) across all populations. The results showed that STAT5A|AvaI loci had monomorphic C allele. Heterozygosity values were found low at both exons 3 and 4 of PRL gene in all population. Sequence analysis results of PRL gene both for exons 3 and 4 showed that there was a mutation between adenine (A) and guanine (G) bases in the RsaI recognized site, whereas in STAT5A gene we can confirm the existence of AvaI restriction site (C|CCGAG).

A Prl/RsaI Polymorphism in Exon 3 and 4 of Prolactin Gene in Dairy Cattle

“…However, it is detected that the DNA sequence of the region examined is similar to exon 4. Furthermore, in the similar studies conducted by different researchers, the exon 3 and exon 4 regions have been shown to produce 156 bp and 294 bp fragment of the Prl gene, respectively, but different genotype frequencies have been determined (Dayal Das et al, 2012;Paramitasari et al, 2015;Sonmez and Ozdemir, 2015). Although the both primer pairs indicating the same region have been studied together in these studies, the observation that the gene and genotype frequencies are found to be different can probably be explained as an experimental or sampling error.…”

Investigating new SNPs of CAST, CAPN and SCD genes in 5’UTR of Bali cattle

“…The investigation of SNPs in 5'UTR, especially the SNPs g.232G>T and g.1890A>del in the CAPN and SCD genes, can be a key for future research; they are specific to Bali cattle with high polymorphism. SNPs that are found in beef cattle (Bos taurus) applied in Bali cattle (Bos javanicus) and displayed low diversity (monomorphic), as observed in the growth hormone receptor (GHR) gene (Zulkharnaim et al 2010), growth hormone (GH) gene (Jakaria and Noor 2011), STAT5 gene (Paramitasari et al 2015), and ADIPOQ and EDG1 genes (Sutikno et al 2018), including 5'UTR region in the EDG1 gene. Developing SNPs as genetic markers for the CAPN and SCD genes of Bali cattle offers a promising method to increase the economic value of the cattle, primarily in producing high-quality meat, especially meat tenderness and fatty acid composition.…”

Peningkatan Limfosit, Monosit, dan Basofil pada Sapi Bali yang Digembalakan di Tempat Pembuangan Akhir Sampah Kota Denpasar (THE ELEVATION LEVEL OF LIMPHOCYTE, MONOCYTE, AND BASOPHIL ON BALI CATTLE GRAZING AT WASTE DISPOSAL CITY OF DENPASAR)

“…Saat ini populasi sapi bali di Provinsi Bali mencapai 637.800 ekor (Disnak Keswan.Bali, 2013). Sapi bali mempunyai potensi genetik yang dapat dijadikan salah satu dasar dalam pelaksanaan program pengembangan sapi bali di seluruh Indonesia (Istiqomah et al, 2010;Septian et al, 2015;Paramita et al, 2015).…”