The Genetic Structure of the Field Pea Landrace “Roveja di Civita di Cascia”

Abstract

“Roveja di Civita di Cascia” is a landrace of Pisum sativum grown in marginal land habitats of the Apennines, Central Italy, and is one of the eleven herbaceous crop landraces listed in the Regional Register of local varieties. The objective of the present paper was to assess its genetic structure using 62 morphological traits and five microsatellites. As many as 55 traits showed significant differences with the control entries (P. sativum subsp. sativum var. arvense and var. sativum). We tested P. sativum Simple Sequence Repeats (SSRs) for their transferability to “Roveja”, and found that only 12 out of 35 performed well. Of these, we demonstrated that five were sufficient to assess the genetic structure of this landrace, characterized by several private alleles, differentiating it from Paladio and Bluemoon, which were used as controls. Phenotypic and genotypic data evidenced a genetic structure based on a blend of several pure-bred lines. The sustainability of on-farm landrace conservation is discussed.

Genetic Characterization and Population Structure of Pea (Pisum sativum L.) by Molecular Markers against Rust (Uromyces viciae-fabae) in Newly Developed Genotypes

“…However, the genetic variance explained among the population was low (24%) regardless of their geographical distance that may be due to an increase in the spread of alleles among various populations. Similar low variance among the population was also reported by Ram et al [26] and Ferradini et al [37].…”

“…Ahmad et al [36] found four groups for 34 pea genotypes of different origins. However, Ferradini et al [37] noticed two peaks at delta K graph, i.e.,K = 2. Hanci and Cebeci [38] evaluated wild pea accessions, local varieties, and commercial pea varieties.…”

“…This genetic differentiation among the individuals in the population may be due to the inclusion of genotypes from the different places of origin for rust resistance. Ferradini et al [37] made a brief account of pea genotypes and found 68% genetic diversity among individuals. Alike, Scaerano et al [35] found 68% genetic difference among the landraces and 32% within the landraces of common bean.…”

Discrimination of Potato (Solanum tuberosum L.) Accessions Collected in Majella National Park (Abruzzo, Italy) Using Mid-Infrared Spectroscopy and Chemometrics Combined with Morphological and Molecular Analysis

“…The genetic resources of a specific territory are generally characterized by morphological descriptors that are useful marker types accepted by the International Union for Protection of New Varieties of Plants [6]. However, many morphological characters are influenced by environmental factors; for this reason, the genetic characterization of the local germplasm is assessed via a combination of morphological and molecular markers [7,8].…”