Genetic Characterization and Population Structure of Pea (Pisum sativum L.) by Molecular Markers against Rust (Uromyces viciae-fabae) in Newly Developed Genotypes

Abstract

The understanding of the genetic diversity of germplasm of any crop is necessary for genetic improvement. Pea (Pisum sativum L.) is a very important legume crop that provides protein and several essential vitamins, carbohydrates, and minerals. The genetic diversity and population structure of pea germplasm consisted of 115 entries of Australian accessions and 4 entries of Indian varieties used as checks with varying responses and severities of rust, which were analysed using 31 polymorphic SSR (Simple Sequence Repeats) markers. The combination of the markers revealed that 78 alleles were present at 32 loci. It was also observed that each marker had three alleles with an average PIC (Polymorphic Information Content) value of 0.272. The population structure analysis showed the genetic differentiation of the entries. The model-based population structure grouped the entries into three sub-populations of SP1, SP2, and SP3 having 37, 35, and 32 entries, respectively with 15 entries as admixtures. AMOVA (Analysis of Molecular Variance) disclosed that there was 56% variation among the individuals and 20% within the population. A mean fixation index (Fst) of 0.240 among the pea entries exhibited relatively significant variation in population. This study provides basic information to select parental lines for developing rust resistant varieties to meet the ultimate goal of sustainable agriculture.

Rust (Uromyces viciae-fabae Pers. de-Bary) of Pea (Pisum sativum L.): Present Status and Future Resistance Breeding Opportunities

“…Now, the classification of resistant and susceptible is also based on microscopic observations of pre-penetration events and the development of a colony of rust in the host visualized after the tryptan blue staining technique developed by Sillero and Rubiales [46]. Recently, Yadav et al [79] have completed the genetic characterization and population structuring of 119 pea genotypes based on SSR (simple sequence repeat) markers and AUDPC values.…”

Priority directions of modern breeding of vegetable peas (<i>Pisum sativum</i> L.)

“…4). Знания ученых иммунологов о генетике устойчивости расширяются, и теперь у гороха идентифицированы молекулярные маркеры, связанные с генами/QTL (локусами количественных признаков) устойчивости ко многим заболеваниям и абиотическим стрессам [20][21][22][23]. Применение таких знаний по картированию на культуре гороха дает возможность использовать в селекционных программах молекулярные маркеры генов, связанных с устойчивостью ко многим патогенам [24][25][26][27].…”