The genetic structure of endemic plant<i>Pteroceltis tatarinowii</i>by ISSR markers

Xiaohong, 李晓红 Li; Hui, 张慧 Zhang; Deyuan, 王德元 Wang; Li, 张莉 Zhang; Jianwen, 邵剑文 Shao; Xiaoping, 张小平 Zhang

doi:10.5846/stxb201212191825

Cited by 8 publications

(1 citation statement)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hence, the genetic diversity was lower at the population than at the species level. Similar findings were reported in Pteroceltis tatarinowii [ 39 ]. The present study revealed that the genetic diversity estimated by value of H was lower than that calculated by value of I among the four populations of wild ancient tea tree.…”

Section: Discussionsupporting

confidence: 91%

Genetic diversity of the wild ancient tea tree (Camellia taliensis) populations at different altitudes in Qianjiazhai

Wang

Cheng

et al. 2023

PLoS ONE

View full text Add to dashboard Cite

In this study, the genetic diversity and population structure of 4 wild ancient tea tree (Camellia taliensis) populations at different altitudes (2,050, 2,200, 2,350, and 2,500 m) in Qianjiazhai Nature Reserve, Zhenyuan country, Yunnan province, were investigated using EST-SSR molecular markers to compare their genetic variation against altitude. In total, 182 alleles were detected across all loci, ranging from 6 to 25. The top one informative SSR was CsEMS4 with polymorphism information content (PIC) of 0.96. The genetic diversity of this species was high, with 100% of loci being polymorphic, an average Nei’s gene diversity (H) of 0.82, and Shannon’s information index (I) of 1.99. By contrast, at the population level, the genetic diversity of wild ancient tea tree was relatively low, with values of H and I being 0.79 and 1.84, respectively. Analysis of molecular variance (AMOVA) revealed a minor genetic differentiation (12.84%) among populations, and most of the genetic variation (87.16%) was detected within populations. Using population structure analysis, we found that the germplasm of wild ancient tea tree was divided into three groups, and there was a substantial gene exchange among these three groups at different altitudes. Divergent habitats caused by altitudes and high gene flow played important roles in genetic diversity of wild ancient tea tree populations, which will provide new opportunities for promoting their protection and potential utilization.

show abstract

Section: Discussionsupporting

confidence: 91%

Genetic diversity of the wild ancient tea tree (Camellia taliensis) populations at different altitudes in Qianjiazhai

Wang

Cheng

et al. 2023

PLoS ONE

View full text Add to dashboard Cite

show abstract

Development and characterization of 20 novel EST‐SSR markers for Pteroceltis tatarinowii, a relict tree in China

Zhang

Wang

et al. 2020

Appl Plant Sci

View full text Add to dashboard Cite

Premise Pteroceltis tatarinowii (Ulmaceae), the only species of the genus Pteroceltis, is an endangered tree in China. Here, novel expressed sequence tag–simple sequence repeat (EST‐SSR) markers were developed to illuminate its genetic diversity for conservation and assisted breeding. Methods and Results Based on Illumina transcriptome data from P. tatarinowii, a total of 70 EST‐SSR markers were initially designed and tested. Forty‐eight of 70 loci (68.6%) were successfully amplified, of which 20 were polymorphic. The number of alleles per locus ranged from two to six, and the levels of observed and expected heterozygosity ranged from 0.018 to 0.781 and from 0.023 to 0.702, respectively. Additionally, cross‐amplification was successful for 17 loci in two related species, Ulmus gaussenii and U. chenmoui. Conclusions These new EST‐SSR markers are valuable transcriptomic resources for P. tatarinowii and will facilitate population genetics and molecular breeding of this species and its relatives in Ulmaceae.

show abstract

Low within-population genetic diversity and high genetic differentiation among populations of the endangered plant Tetracentron sinense Oliver revealed by inter-simple sequence repeat analysis

Gan

et al. 2018

Annals of Forest Science

View full text Add to dashboard Cite

& Key message Tetracentron sinense Oliver, an endangered species from China, displays a low within-population genetic diversity and high genetic differentiation among populations, and the existing populations could be divided into three conservation and management units. & Context The endangered tree Tetracentron sinense Oliver has great value; however, little is known regarding the withinpopulation genetic diversity and differentiation among T. sinense populations. & Aims We examined the genetic diversity and differentiation of T. sinense wild populations, and we tested the effect of small-size population on the level of genetic diversity within these populations. & Methods Using inter-simple sequence repeat (ISSR), we assessed the genetic variation and structure among 174 individuals from 26 natural populations of T. sinense sampled across its distribution range in China. & Results The ISSR primers yielded 180 amplified loci (123 were polymorphic). At the species level, the percentage of polymorphic loci (PPL), Nei's gene diversity (H), and Shannon's information index (I) were 68.3%, 0.196 and 0.300, respectively. The average population level PPL was 20.0%, and the Na, Ne, H, and I were 1.20, 1.13, 0.076, and 0.112, respectively. AMOVA revealed high genetic differentiation among populations (52.0% of total variance, P = 0.001), consistent with the gene differentiation coefficient (Gst = 0.607) and gene flow (Nm = 0.326). The 174 individuals of the 26 T. sinense populations clustered into three groups, and T. sinense geographic and genetic distance were significantly correlated. & Conclusions T. sinense exhibited intermediate within-species genetic diversity, indicating preserved evolutionary potential. The low within-population genetic diversity and high genetic differentiation among T. sinense populations may be one of important factors causing endangerment. Three conservation units were determined based on genetic difference and structure. Inter-population introduction of individuals within units via appropriate propagation and seedling management might be an effective strategy for increasing T. sinense within-population genetic diversity and population size.

show abstract

The genetic structure of endemic plantPteroceltis tatarinowiiby ISSR markers

Cited by 8 publications

References 11 publications

Genetic diversity of the wild ancient tea tree (Camellia taliensis) populations at different altitudes in Qianjiazhai

Genetic diversity of the wild ancient tea tree (Camellia taliensis) populations at different altitudes in Qianjiazhai

Development and characterization of 20 novel EST‐SSR markers for Pteroceltis tatarinowii, a relict tree in China

Low within-population genetic diversity and high genetic differentiation among populations of the endangered plant Tetracentron sinense Oliver revealed by inter-simple sequence repeat analysis

Contact Info

Product

Resources

About