Development and characterization of 20 novel EST‐SSR markers for Pteroceltis tatarinowii, a relict tree in China

Abstract

Premise Pteroceltis tatarinowii (Ulmaceae), the only species of the genus Pteroceltis, is an endangered tree in China. Here, novel expressed sequence tag–simple sequence repeat (EST‐SSR) markers were developed to illuminate its genetic diversity for conservation and assisted breeding. Methods and Results Based on Illumina transcriptome data from P. tatarinowii, a total of 70 EST‐SSR markers were initially designed and tested. Forty‐eight of 70 loci (68.6%) were successfully amplified, of which 20 were polymorphic. The number of alleles per locus ranged from two to six, and the levels of observed and expected heterozygosity ranged from 0.018 to 0.781 and from 0.023 to 0.702, respectively. Additionally, cross‐amplification was successful for 17 loci in two related species, Ulmus gaussenii and U. chenmoui. Conclusions These new EST‐SSR markers are valuable transcriptomic resources for P. tatarinowii and will facilitate population genetics and molecular breeding of this species and its relatives in Ulmaceae.

Genetic diversity and population structure analysis in a large collection of white clover (Trifolium repens L.) germplasm worldwide

“…The markers associate with the non-repetitive regions of the genome and exhibit high mutation rates ( Kalia et al, 2011 ; Morgante, Hanafey & Powell, 2002 ). SSR markers have been widely used to evaluate the genetic variation for various plants, e.g., Medicago truncatula ( Eujayl et al, 2004 ), Lolium multiflorum ( Nie et al, 2019 ), Rhdodendron arboretum ( Sharma et al, 2020 ), Pteroceltis tatarinowii ( Zhang et al, 2020 ).…”

Genetic Diversity and Population Structure of an Arctic Tertiary Relict Tree Endemic to China (Sassafras tzumu) Revealed by Novel Nuclear Microsatellite (nSSR) Markers

“…Furthermore, many previous studies indicated that SSR markers have high cross-species transferability among closely related species in plants [ 7 , 36 , 37 , 39 ]. For the genus Sassafras here, 20 nSSRs from S. randaiense has been successfully transferrable to S. tzumu using one individual [ 7 ]; in our study, the tests of interspecies transferability of the 27 nSSRs from S. tzumu to the other two Sassafras species yielded a transfer success rate of 85.19% (23 loci), and the proportion of polymorphic primers for S. albidum and S. randaiense was relatively high (62.96% and 51.85%, respectively) ( Tables S1 and S2 ).…”