The genetic stock structure of larval and juvenile winter flounder larvae in Connecticut waters of eastern Long Island Sound and estimations of larval entrainment

Abstract

Variability at six microsatellite loci was examined among 536 winter flounder Pseudopleuronectes americanus larvae collected from three locations in eastern Long Island Sound shown to be nursery areas for the species. Substantial genetic differences were seen among the putative source populations and thus appeared to be geographically based. These differences were used to characterize the most likely sources of winter flounder larvae entrained at the Millstone Power Station as well as recruitment to juvenile winter flounder collected in the Niantic River. Samples were classified to the most likely geographical source population both by a conditional maximum likelihood method and by a multi-layer feed-forward neural net trained on the differences in microsatellite allele frequencies. The classification of samples by both methods is compared and discussed in the context of winter flounder management. # 2004 The Fisheries Society of the British Isles

Diet Composition and Feeding Habits of Common Fishes in Long Island Bays, New York

“…Research is needed to link local productivity to competing drivers of density-dependent compensation and predation-based mortality in Winter Flounder. Finally, the conflicting genetic evidence for local population structure (Crivello et al 2004;Buckley et al 2008;O'Leary et al 2013) and against such structure (Wirgin et al 2014) …”

Winter Flounder Larval Genetic Population Structure in Narragansett Bay, RI: Recruitment to Juvenile Young-of-the-Year

“…Winter flounder microsatellite loci were characterized as described in Crivello et al (2004). Genomic DNA was extracted from each sample by the method of Kaplan et al (2001).…”

“…However, tagging studies and recent molecular genetics studies have suggested otherwise. A study using six microsatellite loci in winter flounder from Long Island Sound (Crivello et al 2004) has revealed substantial gene frequency differences among putative source populations over relatively small spatial scales (<5 km). Tagging studies within Narragansett Bay (Saila 1962;Powell 1991) have suggested the presence of three discrete winter flounder spawning groups (East Passage/upper Narragansett Bay, Sakonnet River, Mt.…”

Mitochondrial genomics and Northwestern Atlantic population genetics of marine annelids

“…Johnson and Black 1984, Johnson and Wernham 1999, Drouin et al 2002, Crivello et al 2004, there currently exist no population genetic models that treat stages separately or that consider the possible genetic effects of invertebrate life cycles. Classic population genetics models such as Wright's island model (1943) Kimura and Weiss's stepping stone model (1964), or the phylogeographic method of Avise et al (1987) still provide the basis for estimating gene flow, but new models should be developed to accommodate the new markers, sub-adult samples, and finer-scale questions becoming more common in population genetics.…”

“…Johnson and Wernham (1999) suggest that seasonal differences in the genetic composition of settling larvae could cause settlers to be genetically differentiated from adults (see also Crivello et al 2004). The genetic composition of larvae reaching any particular site would also change in time, which would lead to differentiation in adult populations as well.…”