The Genetic Basis of Morphological Diversity in Domesticated Goldfish

Kon, Tetsuo; Omori, Yoshihiro; Fukuta, Kentaro; Wada, Hironori; Watanabe, Masakatsu; Chen, Zelin; Iwasaki, Miki; Mishina, Tomobumi; Matsuzaki, Shin Ichiro S.; Yoshihara, Daiki; Arakawa, Jumpei; Kikuchi, Kôichi; Toyoda, Atsushi; Burgess, Shawn M.; Noguchi, Hideki; Furukawa, Takahisa

doi:10.1016/j.cub.2020.04.034

Cited by 59 publications

(84 citation statements)

References 113 publications

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“…Although our GWAS analysis identified some genes previously known to be associated with the development of related traits in teleosts, we also identified many loci of unknown function. Very recently, the potentially associated genes relative to the dorsal fin loss phenotype were also identified in goldfish, and lrp6S was found to be located in subgenome S (corresponding to subgenome B in our notation) (SI Appendix, Table S11) (44). Based on classical genetics (crossing experiments), GWAS, and population history analyses in this study, we consider that the dorsal fin trait is likely controlled by multiple loci, as supported by our much wider sampling of goldfish; thus, the discrepancy between the current study and Kon et al (44) is possibly caused by the differences of the two GWAS pupations.…”

Section: Discussionmentioning

confidence: 84%

The evolutionary origin and domestication history of goldfish ( Carassius auratus )

Chen

Zhang

Tang

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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Goldfish have been subjected to over 1,000 y of intensive domestication and selective breeding. In this report, we describe a high-quality goldfish genome (2n = 100), anchoring 95.75% of contigs into 50 pseudochromosomes. Comparative genomics enabled us to disentangle the two subgenomes that resulted from an ancient hybridization event. Resequencing 185 representative goldfish variants and 16 wild crucian carp revealed the origin of goldfish and identified genomic regions that have been shaped by selective sweeps linked to its domestication. Our comprehensive collection of goldfish varieties enabled us to associate genetic variations with a number of well-known anatomical features, including features that distinguish traditional goldfish clades. Additionally, we identified a tyrosine-protein kinase receptor as a candidate causal gene for the first well-known case of Mendelian inheritance in goldfish—the transparent mutant. The goldfish genome and diversity data offer unique resources to make goldfish a promising model for functional genomics, as well as domestication.

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Section: Discussionmentioning

confidence: 84%

The evolutionary origin and domestication history of goldfish ( Carassius auratus )

Chen

Zhang

Tang

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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“…In contrast to eye size expansion resulting from increased retinal cell numbers, zebrafish and goldfish bugeye mutants exhibit enlarged eyes due to increased intraocular pressure (Kon et al, 2020;Stujenske et al, 2011;Veth et al, 2011). The bugeye phenotype is adult-onset and shows retinal cell density decrease and thinning of all nuclear layers.…”

Section: Enlargement Of the Eye Had Been Reported In Few Experimentalmentioning

confidence: 99%

Igf signaling couples retina growth with body growth by modulating progenitor cell division

2021

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How the body and organs balance their relative growth is of key importance for coordinating size and function. This is of particular relevance in organisms, which continue to grow over their entire life span. We addressed this issue in the neuroretina of medaka fish (Oryzias latipes), a well-studied system to address vertebrate organ growth. We reveal that a central growth regulator, Igf1 receptor (Igf1r), is necessary and sufficient for proliferation control in the postembryonic retinal stem cell niche, the ciliary marginal zone (CMZ). Targeted activation of Igf1r signaling in the CMZ uncouples neuroretina growth from body size control, and we demonstrate that Igf1r operates on progenitor cells stimulating their proliferation. Activation of Igf1r signaling increases retinal size while preserving its structural integrity, revealing a modular organization where progenitor differentiation and neurogenesis are self-organized and highly regulated. Our findings position Igf signaling as key module for controlling retinal size and composition with important evolutionary implications.

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“…In this paper, we propose the use of the goldfish heart as an alternative novel experimental model to study excitation–contraction coupling. Goldfish ( Carassius auratus ) belong to the same family as zebrafish ( Málaga-Trillo et al, 2002 ; Kon et al, 2020 ), presenting many physiological similarities. Goldfish hearts are similar to zebrafish hearts, presenting similar advantageous characteristics, such as the possibility of being used as an embryological model ( Grivas et al, 2014 ), being altered via transgenesis using CRISPR/Cas9 ( Yin et al, 2018 ), or being used in electrophysiological and Ca 2+ signaling experiments ( Chen et al, 2005 ; Leo et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Excitation–Contraction Coupling in the Goldfish (Carassius auratus) Intact Heart

Bazmi

Escobar

2020

Front. Physiol.

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Cardiac physiology of fish models is an emerging field given the ease of genome editing and the development of transgenic models. Several studies have described the cardiac properties of zebrafish ( Denio rerio ). The goldfish ( Carassius auratus ) belongs to the same family as the zebrafish and has emerged as an alternative model with which to study cardiac function. Here, we propose to acutely study electrophysiological and systolic Ca 2+ signaling in intact goldfish hearts. We assessed the Ca 2+ dynamics and the electrophysiological cardiac function of goldfish, zebrafish, and mice models, using pulsed local field fluorescence microscopy, intracellular microelectrodes, and flash photolysis in perfused hearts. We observed goldfish ventricular action potentials (APs) and Ca 2+ transients to be significantly longer when compared to the zebrafish. The action potential half duration at 50% (APD 50 ) of goldfish was 370.38 ± 8.8 ms long, and in the zebrafish they were observed to be only 83.9 ± 9.4 ms. Additionally, the half duration of the Ca 2+ transients was also longer for goldfish (402.1 ± 4.4 ms) compared to the zebrafish (99.1 ± 2.7 ms). Also, blocking of the L-type Ca 2+ channels with nifedipine revealed this current has a major role in defining the amplitude and the duration of goldfish Ca 2+ transients. Interestingly, nifedipine flash photolysis experiments in the intact heart identified whether or not the decrease in the amplitude of Ca 2+ transients was due to shorter APs. Moreover, an increase in temperature and heart rate had a strong shortening effect on the AP and Ca 2+ transients of goldfish hearts. Furthermore, ryanodine (Ry) and thapsigargin (Tg) significantly reduced the amplitude of the Ca 2+ transients, induced a prolongation in the APs, and altogether exhibited the degree to which the Ca 2+ release from the sarcoplasmic reticulum contributed to the Ca 2+ transients. We conclude that the electrophysiological properties and Ca 2+ signaling in intact goldfish hearts strongly resembles the endocardial layer of larger mammals.

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The Genetic Basis of Morphological Diversity in Domesticated Goldfish

Cited by 59 publications

References 113 publications

The evolutionary origin and domestication history of goldfish ( Carassius auratus )

The evolutionary origin and domestication history of goldfish ( Carassius auratus )

Igf signaling couples retina growth with body growth by modulating progenitor cell division

Excitation–Contraction Coupling in the Goldfish (Carassius auratus) Intact Heart

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