The genetic architecture of 3′untranslated region of the MICA gene: Polymorphisms and haplotypes

Luo, Jia; Tian, Wei; Liu, Xue Xiang; Yu, Jun; Li, Lixin; Pan, Fenghua

doi:10.1016/j.humimm.2013.06.024

Cited by 8 publications

(20 citation statements)

References 18 publications

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“…There are currently nine known polymorphic positions within the MICA 3'UTR as determined by Luo et al, which form 7 distinct 3'UTR types designated MICA‐UTR1‐7, as detailed in Table and Figure , showing polymorphic positions or full sequence, respectively. Among these, MICA‐UTR1, −2, −4, and −7 were identified in IHW reference cell line DNA in this study.…”

Section: Resultssupporting

confidence: 93%

“…MICA‐UTR5 and −6 were also not observed in our study, possibly because of being rare as these types were only seen in one individual each in the previous study. In accordance with the previous study where MICA‐UTR1 and UTR2 had allele frequencies of 70% and 24%, respectively, MICA‐UTR1 was observed most often, followed by UTR2, in this study.…”

Section: Resultsmentioning

confidence: 92%

“…Among these, MICA‐UTR1, −2, −4, and −7 were identified in IHW reference cell line DNA in this study. MICA‐UTR3 was assigned to the MICA null haplotype and had a relatively high allele frequency of 4% in the previous study of Chinese Han individuals but was not observed in our study of mostly European IHW reference DNA samples. MICA‐UTR5 and −6 were also not observed in our study, possibly because of being rare as these types were only seen in one individual each in the previous study.…”

Section: Resultsmentioning

confidence: 95%

“…The purpose of this study was to investigate polymorphism of the 3'UTR of MICA and MICB genes in European IHW cell line DNA, as previous studies have focussed on a specific population of Chinese Han . We previously investigated the 5'UTR of MICA and MICB using the same reference DNA samples and this study extends those findings and provides a more complete picture of the relationship between 5'UTR promoter, coding and 3'UTR polymorphisms and implications for ligand expression levels and functional potential of the various MICA/B alleles.…”

Section: Discussionmentioning

confidence: 95%

“…We identified 17 MICA extended haplotypes when considering the allele type and 3'UTR polymorphism. Four haplotypes were unique to this study among IHW DNA and together with the 21 haplotypes already described, a total of 25 are now known. Eleven MICB extended haplotypes were found in this study, including five unique among IHW reference DNA.…”

Section: Discussionmentioning

confidence: 98%

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Diversity and characterisation of polymorphic 3' untranslated region haplotypes of MICA and MICB genes

Cox

Hernandez

Danby

et al. 2018

HLA

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MICA and MICB genes encode ligands that interact with the natural killer (NK) cell activating receptor, NKG2D. These ligands display a highly polymorphic allelic repertoire, although the true functional significance of this polymorphism remains elusive. We previously reported additional polymorphism in the 5' untranslated region (UTR) proximal promoter region of these genes by sequencing international histocompatibility workshop (IHW) cell line DNA promoter and coding regions. The present study extends this analysis by further characterising the 3'UTR region of the same IHW reference panel to achieve a more complete understanding of MICA and MICB haplotype diversity and possible functional relevance. We found 17 extended MICA haplotypes encompassing the coding region and 3'UTR, including four novel haplotypes identified in IHW cell line DNA. This increased to 21 when also considering the 5'UTR proximal promoter region. Analysis of the MICB 3'UTR revealed two novel sequences in cell lines KLO and WIN designated MICB‐UTR8 and UTR9, respectively. A total of 11 MICB haplotypes were identified in this study and five were unique. The present study, characterising MICA/B 3'UTR polymorphism utilising IHW reference cell lines, could be useful for future studies investigating the role of microRNA in post‐transcriptional repression of gene expression and for immunotherapy strategies to combat cancer progression.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 92%

Section: Resultsmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 98%

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