The Gene Organization, Chromosome Location, and Expression of a 55-kDa Matrix Protein (PRELP) of Human Articular Cartilage

Grover, Judy; Chen, Xiao Ning; Korenberg, Julie R.; Recklies, Anneliese D.; Roughley, Peter J.

doi:10.1006/geno.1996.0605

Cited by 101 publications

(128 citation statements)

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“…Goat polyclonal antibodies to fibromodulin were obtained from Santa Cruz Biotechnology. Rabbit polyclonal antibodies to lumican and biglycan were prepared as described previously (29,30). Blots were then treated with the appropriate peroxidaseconjugated secondary antibody: donkey anti-goat (Jackson) at 1:5000 dilution or donkey anti-rabbit (Santa Cruz) at 1:2000 dilution.…”

Section: Methodsmentioning

confidence: 99%

Analysis of Intimal Proteoglycans in Atherosclerosis-prone and Atherosclerosis-resistant Human Arteries by Mass Spectrometry

Talusan

Bedri

Yang

et al. 2005

Molecular & Cellular Proteomics

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The propensity to develop atherosclerosis varies markedly among different sites in the human vasculature. To determine a possible cause for such differences in atherosclerosis susceptibility, a proteomics-based approach was used to assess the extracellular proteoglycan core protein composition of intimal hyperplasia from both the atherosclerosis-prone internal carotid artery and the atherosclerosis-resistant internal thoracic artery. The intimal proteoglycan composition in these preatherosclerotic lesions was found to be more complex than previously appreciated with up to eight distinct core proteins present, including the large extracellular proteoglycans versican and aggrecan, the basement membrane proteoglycan perlecan, the class I small leucine-rich proteoglycans biglycan and decorin, and the class II small leucine-rich proteoglycans lumican, fibromodulin, and prolargin/ PRELP (proline arginine-rich end leucine-rich repeat protein). Although most of these proteoglycans seem to be present in similar amounts at the two locations, there was a selective enhanced deposition of lumican in the intima of the atherosclerosis-prone internal carotid artery compared with the intima of the atherosclerosis-resistant internal thoracic artery. The enhanced deposition of lumican in the intima of an atherosclerosis prone artery has important implications for the pathogenesis of atherosclerosis.

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Section: Methodsmentioning

confidence: 99%

Analysis of Intimal Proteoglycans in Atherosclerosis-prone and Atherosclerosis-resistant Human Arteries by Mass Spectrometry

Talusan

Bedri

Yang

et al. 2005

Molecular & Cellular Proteomics

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“…Because these class-II small leucine-rich proteoglycans are demonstrated to function in regulating collagen fibril growth in various connective tissues, 19,20 PRELP may have a similar function in intraarticular connective tissues. Grover et al 21 characterized the expression pattern and genomic structure of human PRELP. The messages of PRELP are abundant in cartilage, but are not present in cultured fibroblasts.…”

Section: Similar Gene Expressions Of Intraarticular-mscsmentioning

confidence: 99%

Mesenchymal stem cells derived from synovium, meniscus, anterior cruciate ligament, and articular chondrocytes share similar gene expression profiles

Segawa

Muneta

Makino

et al. 2009

Journal Orthopaedic Research

188

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Mesenchymal stem cells (MSCs) can be obtained from various tissues, and contain common features. However, an increasing number of reports have described variant properties dependent of cell sources. We examined (1) whether MSCs existed in several intraarticular tissues, (2) whether gene expression profiles in intraarticular tissue MSCs closely resembled each other, and (3) whether identified genes were specific to intraarticular tissue MSCs. Human synovium, meniscus, intraarticular ligament, muscle, adipose tissue, and bone marrow were harvested, and colony-forming cells were analyzed. All these cells showed multipotentiality and surface markers typical of MSCs. Gene profiles of intraarticular tissue MSCs and chondrocytes were closer to each other than those of extraarticular tissues MSCs. Among three characteristic genes specific for intraarticular tissue MSCs, we focused on proline arginine-rich end leucine-rich repeat protein (PRELP). Higher expression of PRELP was confirmed in chondrocytes and intraarticular tissue MSCs among three elderly and three young donors. Synovium MSCs stably expressed PRELP, contrarily, bone marrow MSCs increased PRELP expression during in vitro chondrogenesis. In conclusion, MSCs could be isolated from various intraarticular tissues including meniscus and ligament, gene expression profiles of intraarticular tissue MSCs closely resembled each other, and the higher expression of PRELP was characteristic of intraarticular tissue MSCs. ß

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“…This early work also revealed that lumican belongs to the family of small leucine-rich repeat proteoglycans (SLRPs), which constitute an important fraction of noncollagenous extracellular matrix (ECM) proteins (2). It has now been established that lumican is expressed in a variety of tissues, including skin (3), artery (4), lung (5), invertebral discs (6), kidney (7), bone (8), aorta (9), and articular cartilage (10). In these tissues lumican may exist in a glycoprotein form, being substituted with short oligosaccharides or poorly sulfated/unsulfated polylactosamine chains rather than KS.…”

Section: Introductionmentioning

confidence: 99%

“…In these tissues lumican may exist in a glycoprotein form, being substituted with short oligosaccharides or poorly sulfated/unsulfated polylactosamine chains rather than KS. Furthermore, it has been demonstrated in some tissues that the structure of the carbohydrate substituents of lumican varies with age (3,10).…”

Section: Introductionmentioning

confidence: 99%

Lumican, a small leucine‐rich proteoglycan

et al. 2008

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SummaryLumican belongs to the family of small leucine-rich repeat proteoglycans. Recent studies have shown that lumican participates in the maintenance of tissue homeostasis and modulates cellular functions including cell proliferation, migration, and differentiation. The expression of lumican has been correlated to the growth and metastasis of various malignancies; however, its exact role in tumorogenesis remains elusive. This review focuses upon the role of lumican in cell biology, providing insights into molecular mechanisms that lumican likely utilizes to control processes relevant to tumorogenesis. IUBMBIUBMB Life, 60(12): 818-823, 2008

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The Gene Organization, Chromosome Location, and Expression of a 55-kDa Matrix Protein (PRELP) of Human Articular Cartilage

Cited by 101 publications

References 1 publication

Analysis of Intimal Proteoglycans in Atherosclerosis-prone and Atherosclerosis-resistant Human Arteries by Mass Spectrometry

Analysis of Intimal Proteoglycans in Atherosclerosis-prone and Atherosclerosis-resistant Human Arteries by Mass Spectrometry

Mesenchymal stem cells derived from synovium, meniscus, anterior cruciate ligament, and articular chondrocytes share similar gene expression profiles

Lumican, a small leucine‐rich proteoglycan

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