The gene, expression pattern and subcellular localization of chitin synthase B from the insect Ostrinia furnacalis

A novel alternative splicing site of class A chitin synthase from the insect Ostrinia furnacalis – Gene organization, expression pattern and physiological significance

“…Until now, insect CHSB is known to have no alternative splicing sites, which is in good agreement with CHSB's restricted expression in midgut during the formation of peritrophic membrane at feeding stage (Arakane et al, 2004Bolognesi et al, 2005;Kato et al, 2006;Kumar et al, 2008;Qu et al, 2011). Unlike CHSB, insect CHSA has been revealed to contain one alternative splicing site in the middle of ORF region.…”

“…furnacalis (Asian corn borer) larvae used in this study were reared in an artificial diet as described previously (Qu et al, 2011).…”

Maize toxin degrades peritrophic matrix proteins and stimulates compensatory transcriptome responses in fall armyworm midgut

“…Our targeted and global transcriptome analyses detected differential expression of MG transcripts from genes encoding proteins that function in PM production. These proteins include chitin synthase and chitin deacytlases known to be involved in the production of PM chitin fibrils (Bolognesi et al, 2005;Toprak et al, 2008;Qu et al, 2011). They also include IIMs and other peritrophins with chitin-binding domains that crosslink chitin fibrils in the PM (Toprak et al, 2010a,b).…”

Fully Deacetylated Chitooligosaccharides Act as Efficient Glycoside Hydrolase Family 18 Chitinase Inhibitors

“…furnacalis larvae were reared using an artificial diet under a photo period of 16 h light and 8 h darkness and a relative humidity of 70 -90% at 26 -28°C as described previously (41). In the experimental group, 100 g of mixed (GlcN) 2-7 that was dissolved in 2 l of distilled water was injected into the penultimate abdominal segment of the fifth instar day 5 larvae using a microsyringe.…”