The gelatin particle indirect agglutination test, a means of simple and sensitive serodiagnosis of Chagas' disease.

Abstract

Abstract:An indirect agglutination test using a Trypanosoma cruzi antigen-coated gelatin particles was employed to diagnose trypanosomiasis in Paraguay.Results with this test were quite comparable to those obtained with enzyme-linked immunosorbent assay (ELISA) . Furthermore, nonspecific reaction to the gelatin particles alone was not found in either acute or chronic infection. This method is more convenient than the ELISA, since the antigen-conjugated particles is stable for at least 1 year at 4°C and since the test itself is short and simple to perform and does not require specialized equipment.

Application of The Gelatin Particle Indirect Agglutination Test in The Serodiagnosis of Human Opisthorchiosis.

“…When it is used routinely, a suitable serum dilution (1: 60) , a gel particle control, a control antiserum of known titer and a negative control should be included with each test run. This study also confirmed previous works (Sato and Ryumon, 1990;Yamashita et al, 1994;Yang et al, 1994;Kobayashi et al, 1995) that GPAT is much simpler because it is a one-step reaction between the antigen-coated particles and the test serum, and is indeed simple for an ordinary laboratory or for a field. survey to carry out for the mass screening of opisthorchiosis.…”

“…The test was performed in an U-shaped bottom microtiter plate (Falcon, Becton Dickinson and Co., USA) as described elsewhere (Sato and Ryumon, 1990;Yamashita et al, 1994) . One drop containing 25 ,u1 of 1% NRS in PBS was placed into each well using an automatically calibrated syringe dispenser (Nichiryo, Model 8100, Nichiryo Co., Japan) .…”

Detection of antibodies to Parastrongylus cantonensis in human sera by gelatin particle indirect agglutination test.

“…The recently developed inert gelatin particles are actively being employed as an antigen carrier for various diagnostic kits, and GPAT has already been successfully applied by a number of investigators for immunodiagnosis of various parasitic infections (Sato and Ryumon, 1990;Yamashita et al, 1994;Yang et al, 1994;Kobayashi et al, 1995;Wattanakulpanich et al, 1998) . In the present study, we confirmed the findings that GPAT could also be used for immunodiagnosis of human parastrongyliasis.…”

“…The test, nevertheless, requires specific materials, specialized equipment and expensive reagents. Recently, a newly developed agglutination test using gelatin particle as an antigen carrier has been shown to be a sensitive and specific method for the diagnosis of human strongyloidiasis (Sato and Ryumon, 1990) , schistosomiasis (Yang et al, 1994;Kobayashi, 1995) , Chagas' disease (Yamashita et al, 1994) and opisthorchiasis (Watthanakulpanich at al., 1998) . GPAT is technically simple and can be performed rapidly without specialized apparatus or facilities.…”