Detection of antibodies to Parastrongylus cantonensis in human sera by gelatin particle indirect agglutination test.

Eamsobhana, Praphathip; Watthanakulpanich, Dorn; Punthuprapasa, Paibulaya; Yoolek, Adisakdi; Suvuttho, Somkuan

doi:10.2149/tmh1973.27.1

Cited by 6 publications

(11 citation statements)

References 11 publications

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“…Consequently, over the past decades a great number of immunological tests have been developed to enable the diagnosis of this human angiostrongyliasis [13], [14]. These approaches include an Indirect Enzyme Linked Immunosorbent Assay (ELISA) using a 31-kDa glycoprotein from the adult worm [8], [15], [16].…”

Section: Introductionmentioning

confidence: 99%

“…The suspected diagnosis can only be confirmed upon finding and identification of A. cantonensis worms from the cerebrospinal fluid of infected patients, but this rarely occurs [10] – [12] . Consequently, over the past decades a great number of immunological tests have been developed to enable the diagnosis of this human angiostrongyliasis [13] , [14] . These approaches include an Indirect Enzyme Linked Immunosorbent Assay (ELISA) using a 31-kDa glycoprotein from the adult worm [8] , [15] , [16] .…”

Section: Introductionmentioning

confidence: 99%

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Seroprevalence of Angiostrongylus cantonensis in Wild Rodents from the Canary Islands

et al. 2011

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Background Angiostrongylus cantonensis is a lungworm of rats (Muridae) that is the causative agent of human cerebral angiostrongyliasis. The life cycle of A. cantonensis involves rats and mollusks as the definitive and intermediate hosts, respectively. This study was designed to increase the knowledge about the occurrence and distribution of A. cantonensis in its definitive host in the Canary Islands, using parasitological and serological analysis in different areas and age groups.Methodology/Principal FindingsBetween 2009 and 2010, 54 black rats (Rattus rattus) from Tenerife were captured from six human-inhabited areas and sera samples were obtained. The lung nematodes were identified by morphological and molecular tools as A. cantonensis. The 31-kDa glycoprotein antigen was purified from A. cantonensis adult worms by electrophoresis and electroelution. Of the 54 tested rodents, 30 showed IgG antibodies against A. cantonensis 31-kDa antigen by ELISA. Therefore, the overall seroprevalence was 55.6% (95% CI: 42.4–68). Seroprevalent rodents were found in all the 6 areas. This 31-kDa antigen was not recognized by some sera of rats infected by other helminth species (but not A. cantonensis). Seroprevalence of IgG antibodies against A. cantonensis and prevalence based on the presence of adult worms showed significant correlation (R2 = 0.954, p<0.05).Conclusions/SignificanceThe present results could indicate a high prevalence of A. cantonensis in Tenerife and suggest the inclusion of two new zones in the distribution area of the parasite. The commonness and wide distribution of A. cantonensis in rats implies the presence of intermediate hosts, indicating that humans may be at risk of getting infected.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Seroprevalence of Angiostrongylus cantonensis in Wild Rodents from the Canary Islands

et al. 2011

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“…Indirect ELISA. ELISA was based on the methods described in Eamsobhana et al 19 with the following modifications: optimal concentration of antigen and serum (positive control) was determined by checkerboard titration. A concentration of 1:200 for primary antibody was derived by titration of positive and negative serum controls with a range of 1:100 to 1:1,000.…”

Section: Methodsmentioning

confidence: 99%

Estimating Human Exposure to Rat Lungworm (Angiostrongylus cantonensis) on Hawai’i Island: A Pilot Study

Jarvi

Eamsobhana

Quarta

et al. 2020

The American Journal of Tropical Medicine and Hygiene

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Angiostrongylus cantonensis is a zoonotic, parasitic nematode causing angiostrongyliasis or rat lungworm disease. Clinical diagnosis in humans is currently confirmed by detection of parasite DNA in cerebrospinal fluid. This study estimated human exposure to A. cantonensis in volunteer participants solicitated via public venues on east Hawai'i Island using blood-based tests. Antibodies were screened in sera by crude antigen ELISA, followed by a 31-kDa dot-blot test developed and validated in Thailand. Human participants (n = 435) donated blood samples and completed a questionnaire to self-report relevant symptomology or clinical diagnosis. Among symptoms reported by participants diagnosed by licensed clinicians, headaches, high eosinophil counts, stiff neck, fatigue, and joint pain were most severe during the initial 3 months of infection. ELISA results revealed 22% of the serum samples as positive, 46% as equivocal, and 32% as negative. A subset of 186 samples was tested by dot blot, with 30% testing positive and 70% testing negative. A significantly higher mean ELISA value was found among recently (2014)(2015) clinically diagnosed participants as than among those with a diagnosis before 2010 (P = 0.027). All dot-blot positives were also ELISA positive and were significantly associated with higher ELISA values compared with dot-blot negatives (P = 0.0001). These results suggest that an ELISA using crude antigen isolated from adult A. cantonensis from Hawai'i may be an effective initial screening method for estimating exposure to A. cantonensis in Hawai'i and likewise suggest that dot-blot tests using the 31-kDa antigen exhibit efficacy as a diagnostic for exposure.

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“…The 31-kDa proteins were first evaluated in 1997, 50 and subsequently shown to have sensitivity and specificity of 100% for detection of antibodies against A. cantonensis . 51 Serological analysis of CSF or serum was compared with real-time PCR and found that the immunodiagnosis was in agreement for 24 of the 26 patients. 49 However, this study 49 used crude antigen ELISA and Western blots (WB), not the isolated 31-kDa proteins.…”

Section: Introductionmentioning

confidence: 99%

“… 54 The gel-isolated and purified 31-kDa antigen has been used most consistently in studies conducted in Thailand. 55 – 59 In these Thailand trials, the glycoproteins comprising the purified 31-kDa antigen have shown 100% sensitivity and 98.72% specificity, and a rapid test kit has been patented. 59 , 60 …”

Section: Introductionmentioning

confidence: 99%

Immunodiagnostic Detection of Angiostrongylus cantonensis Exposure on Hawaii Island Using Isogeographic 31-kDa Antigen

Jarvi

Nakayama

Eamsobhana

et al. 2023

The American Journal of Tropical Medicine and Hygiene

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Angiostrongylus cantonensis is the leading cause of neuroangiostrongyliasis worldwide, and east Hawaii Island is a hotspot for the disease in the United States. A combination of glycoproteins with molecular weight of 31 kDa has been used as antigen to evaluate antibody response in human serum samples in Thailand with high specificity and sensitivity. In a previous pilot study, the Thailand-isolated 31-kDa proteins showed efficacy in dot-blot tests using serum samples from 435 human volunteers on Hawaii Island. However, we hypothesized that native antigen isolated from Hawaii A. cantonensis may exhibit higher specificity than the Thailand-isolated 31-kDa antigen due to potential minor variation in epitopes between isolates. In this study, 31-kDa glycoproteins were isolated by sodium dodecyl-sulfate polyacrylamide gel electrophoresis from adult A. cantonensis nematodes collected from rats captured on east Hawaii Island. The resultant proteins were purified by electroelution, pooled, bioanalyzed, and quantified. A subset of 148 samples from human participants of the original cohort of 435 was consented for this study, including 12 of the original 15 clinically diagnosed participants. Results of ELISA using the Hawaii-isolated 31-kDa antigen were compared with results of the same serum samples previously tested with both crude Hawaii antigen ELISA and Thailand 31-kDa antigen dot blot. This study shows a seroprevalence in the general population of East Hawaii Island of 25.0%, similar to previous findings of 23.8% seroprevalence in this cohort using crude antigen from Hawaii A. cantonensis and 26.5% using Thailand 31-kDa antigen.

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Detection of antibodies to Parastrongylus cantonensis in human sera by gelatin particle indirect agglutination test.

Cited by 6 publications

References 11 publications

Seroprevalence of Angiostrongylus cantonensis in Wild Rodents from the Canary Islands

Seroprevalence of Angiostrongylus cantonensis in Wild Rodents from the Canary Islands

Estimating Human Exposure to Rat Lungworm (Angiostrongylus cantonensis) on Hawai’i Island: A Pilot Study

Immunodiagnostic Detection of Angiostrongylus cantonensis Exposure on Hawaii Island Using Isogeographic 31-kDa Antigen

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