The gastric microbiota in patients with Crohn’s disease; a preliminary study

Ostrowski, Jerzy; Kulecka, Maria; Zawada, Iwona; Żeber‐Lubecka, Natalia; Paziewska, Agnieszka; Graca-Pakulska, Katarzyna; Dąbkowski, Krzysztof; Skubisz, Karolina; Cybula, Patrycja; Ambrożkiewicz, Filip; Urasińska, Elżbieta; Mikula, Michał; Starzyńska, Teresa

doi:10.1038/s41598-021-97261-z

Cited by 5 publications

(3 citation statements)

References 38 publications

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“…This indication is driven by results of our recent molecular study, in which we analyzed the gastric microbiome in H. pylori— negative patients with Crohn’s disease. We observed that in comparison to non-IBD control individuals the gut microbiota profile was significantly altered in patients with Crohn’s disease, with differences noted in beta diversity, bacterial phyla, and individual taxa 19 . We consider that the gastric and duodenal mucosal abnormalities reported in this study may represent a consequence/manifestation of the aforementioned altered microbiome.…”

Section: Discussionmentioning

confidence: 83%

Endoscopic findings in the upper gastrointestinal tract in patients with Crohn’s disease are common, highly specific, and associated with chronic gastritis

Graca-Pakulska

Błogowski

Zawada

et al. 2023

Sci Rep

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Crohn’s disease (CD) may affect the entire gastrointestinal tract including its upper part. However, this aspect is poorly addressed in scientific literature and considered a rare finding. Here we aimed to prospectively investigate the prevalence, characteristics and clinical significance of upper gastrointestinal tract involvement in patients with CD, with particular focus on stomach bamboo joint-like appearance (BJA), Helicobacterpylori status and presence of microscopic changes. 375 prospectively recruited patients were included. In CD patients the prevalence of gastric and duodenal, but not esophageal, mucosal lesions, such as gastric mucosal inflammation, duodenal edema, ulcerations, and duodenal bulb deformation was significantly higher (at least p < 0.01 for all). Similar results were found when only H.pylori negative individuals were analyzed. Moreover, BJA of the stomach and in case of H.pylori negative patients also duodenal bulb deformation were detected exclusively in CD patients. Presence of BJA lesion was not significantly associated with neither duration of the disease nor use/history of biologic treatment. Despite absence of H.pylori infection microscopic features of chronic gastritis were found in almost all (93.5%) patients, and in 31% of controls (p < 0.00001). Our analysis outlines that upper gastrointestinal tract involvement in CD is a very common event and frequently manifests with a highly specific BJA lesion. Furthermore, our study reveals that in almost all CD patients features of H.pylori negative gastritis are present.

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Section: Discussionmentioning

confidence: 83%

Endoscopic findings in the upper gastrointestinal tract in patients with Crohn’s disease are common, highly specific, and associated with chronic gastritis

Graca-Pakulska

Błogowski

Zawada

et al. 2023

Sci Rep

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“…Campylobacter infection usually causes diarrhea, fever, and stomach cramps. Absconditabacteriales exhibited significantly lower abundance in the gastric microbiota in HP− Crohn’s disease patients than in control subjects ( Ostrowski et al, 2021 ). Such findings indicated non-HP pathogens could play a potential role in PCG.…”

Section: Discussionmentioning

confidence: 99%

Gastric juice microbiota in pediatric chronic gastritis that clinically tested positive and negative for Helicobacter pylori

Chen

Xia

et al. 2023

Front. Microbiol.

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PurposeHelicobacter pylori (HP) infection is an identified risk factor for pediatric chronic gastritis (PCG), but its impact on gastric juice microbiota (GJM) remains to be further elucidated in PCG. This study aimed to analyze and compare the microbial communities and microbial interactive networks of GJM in PCG that clinically tested positive and negative for HP (HP+ and HP−, respectively).MethodsA total of 45 PCG patients aged from 6 to 16 years were recruited, including 20 HP+ and 25 HP− patients tested by culture and rapid urease test. Gastric juice samples were collected from these PCG patients and subjected to high-throughput amplicon sequencing and subsequent analysis of 16S rRNA genes.ResultsWhile no significant change in alpha diversity, significant differences in beta diversity were observed between HP+ and HP− PCG. At the genus level, Streptococcus, Helicobacter, and Granulicatella were significantly enriched in HP+ PCG, whereas Campylobacter and Absconditabacteriales (SR1) were significantly enriched in HP− PCG. Network analysis showed that Streptococcus was the only genus positively correlated with Helicobacter (r = 0.497) in the GJM network of overall PCG. Moreover, compared to HP− PCG, HP+ PCG showed a reduction in microbial network connectivity in GJM. Netshift analysis identified driver microbes including Streptococcus and other four genera, which substantially contributed to the GJM network transition from HP− PCG to HP+ PCG. Furthermore, Predicted GJM function analysis indicated up-regulated pathways related to the metabolism of nucleotides, carbohydrates, and L-Lysine, the urea cycle, as well as endotoxin peptidoglycan biosynthesis and maturation in HP+ PCG.ConclusionGJM in HP+ PCG exhibited dramatically altered beta diversity, taxonomic structure, and function, with reduced microbial network connectivity, which could be involved in the disease etiology.

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“…In this study, we analyzed the high-throughput sequencing results of 16S rRNA V3-V4 variable region of oral microbiota in HpAG patients and healthy people.At the phylum level, Proteobacteria, Bacteroidota, Firmicutes and Fusobacteriota were the main salivary bacteria.This research result is consistent with the research of Ansari S [24] .The reliability of our sample and analytical method is confirmed.Proteobacteria is Gram-negative bacteria whose outer membrane is mainly composed of lipopolysaccharides.It is a large group of bacteria in the gastrointestinal tract.Due to its instability, it can easily lead to the destruction of the symbiosis system of bacteria, and then mediate inflammation and immune response [25][26] .Firmicutes and members of Bacteroidota were involved in the regulation of lipid and bile acid metabolism and the maintenance of energy balance of the host [27] .Fusobacteriota causes opportunistic infections and is mostly associated with gastrointestinal diseases.At the same time, it is also a common bacterial species in oral microbial cases and may have a symbiotic relationship with patients [28] .The Top10 species with different abundance at phylum level were selected for relative abundance boxplot analysis.Compared with the other two groups, the relative abundance of Actinobacteriota in HT group was significantly increased.It may be the characteristic flora of spleen and stomach damp-heat syndrome in HpAG patients at the portal level.Studies have found that Actinobacteriota has specific changes in Crohn's disease, and Actinobacteriota has a special microbial composition in gastric juice [29] .…”

Section: Discussionmentioning

confidence: 99%

Composition and diverse differences of salivary microbiota in gastritis correlated to Helicobacter pylori patients

Wang

Huang

Liu

et al. 2022

Preprint

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Objective: To explore the composition and diversity of oral microbiota in patients with Helicobacter pylori associated gastritis (HpAG), and to determine the differences of microbiota between patients with thin white moss and patients with yellow oily moss. Methods: Between September 2021and June 2022, we flew into our study, saliva samples were collected from HC group (healthy control subjects) Hp group (hp-positive thin white moss patients, hp-positive yellow greasy moss patients).The V3-V4 region of 16S rRNA was amplified by PCR. After high-throughput sequencing, the sequencing data were processed for ASV annotation, community structure and diversity analysis. Results: No differences in α diversity were found among the three groups.There were significant differences in the structure of the three groups in β-diversity.ANOSIM test (P = 0.001) showed significant differences between groups.At the phylum level, the relative abundance of Actinobacteriota in HT group was significantly increased, which may be a unique biomarker of Actinobacteriota.At the genus level, the biomarkers of HC group may be Clostridium_sensu_stricto_1 and Escherichia-Shigella.Biomarkers of BT group may be Mycoplasma, Muribaculaceae and Johnsonella.The biomarkers of HT group may be Actinomyces, Atopobium, Lachnoanaerobaculum, Stomatobaculum and Veillonella. Conclusion: There were significant differences in the richness and diversity of salivary microbiota among the three groups.Different bacterial taxa can be used as biomarkers to increase our understanding of the relationship between salivary microbiota and H. pylori associated gastritis.

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The gastric microbiota in patients with Crohn’s disease; a preliminary study

Cited by 5 publications

References 38 publications

Endoscopic findings in the upper gastrointestinal tract in patients with Crohn’s disease are common, highly specific, and associated with chronic gastritis

Endoscopic findings in the upper gastrointestinal tract in patients with Crohn’s disease are common, highly specific, and associated with chronic gastritis

Gastric juice microbiota in pediatric chronic gastritis that clinically tested positive and negative for Helicobacter pylori

Composition and diverse differences of salivary microbiota in gastritis correlated to Helicobacter pylori patients

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