The Further Purification of Motilin, a Gastric Motor Activity Stimulating Polypeptide from the Mucosa of the Small Intestine of Hogs

Abstract

BROWN, J. C., MUTT, V., and DRYBURGH, J. R. 1971. The further purification of motilin, a gastric motor activity stitnulating polypeptide from the mucosa of the small intestine of hogs. Can. J. Physiol. Pharmacol. 49,[399][400][401][402][403][404][405] A polypeptide has been isolated from the duodenal mucosa of hogs and has been named motilin. Motilin stimulates motor activity in both antral and fundic gland area pouches of the stomach of dogs. Pt will stimulate pepsin output, with no change in H' secretion, from fundic gland area pouches. Motilin differs from the other characterized gastrointestinal polypeptides both chemically and in its physiological actions. In particular, the presence of phenylalanine as N-terminal residue, the absence of histidine, and presence of large amounts of glutamic acid and glutamine distinguish it from cholecystokinin-pancreozymin and secretin. BROWN, b. C., MUTT, V.. et DRYRURGII. J . W. 1971. The further purification of motilin, a gastric motor activity stinmulating polypeptide from the nmucosra of the small intestine of hogs. Can. J. Physiol. Pharnmacol. 49, 399-4135.Wous avons is016 B partir de la muqueuse duodenale de porc. un polypeptide que nous avons nomrnk motiline. I1 stimufe la motilitC des poches de I'antre pylorique et du fundus de l'estornac de chien. I1 stirnule kgalernent la libkration de pepsine sans motifier la skcrbtion d'H+ B partir des aires ~Ccrbtrices du fundus. La motiline diffhre des autres polypeptides gastro-intestinaux dbjB connus par caractCristiques chimiques et ses actions physiologiques. En particulier. les caract2res qui le distinguent de la s@cr@tine et du CCK-PZ sont la prCsence de phbnylalanine csmme rksidu N-terminal, l'ahsence d'histidine et l'abondance de glutamine et d'acide glutanmique.

An Ultrastructural Study on the Endocrine Pancreas of Caiman latirostris, with Special Reference to Pancreatic Motilin Cells.

“…Motilin, a 22 amino acid peptide, originally isolated from porcine duodenal mucosa (BROWN et al, 1971), stimulates enteric smooth muscle locally (BROWN et al, 1971;ITOH, 1976;CHRISTOFIDES et al, 1979). It has been well established that motilinimmunoreactive cells are one of the major endocrine cell types in the proximal small intestine of mammals.…”

Effect of insulin on motilin release in man.

“…A significant negative correlation between MLI and IRI concentrations (r = -0.72, p <0.01) was observed. The present results indicate that the suppressive effect of insulin on motilin release is a direct action of insulin and is not mediated by glucose.insulin ; motilinMotilin, a 22 amino-acid peptide hormone has been known as one of the gut hormones which affect the motility of the stomach and intestine (Brown et al 1971;Lee et al 1974;Jennewein et al 1975). It has been shown that intraduodenal administration of glucose and amino-acids solution, which increase insulin release, inhibit the release of endogenous motilin (Christofides et al 1979).…”

“…Motilin, a 22 amino-acid peptide hormone has been known as one of the gut hormones which affect the motility of the stomach and intestine (Brown et al 1971; Lee et al 1974;Jennewein et al 1975). It has been shown that intraduodenal administration of glucose and amino-acids solution, which increase insulin release, inhibit the release of endogenous motilin (Christofides et al 1979).…”

Actions of Motilin on Gastrointestinal Motility and Plasma Immu.noreactive Motilin Concentration in Interdigestive and Postprandial States

“…When motilin, a 22amino acid polypeptide, was originally discovered and isolated from the hog duodenal mucosa by Brown et al in 1971, this hormone candidate was considered as an exciting agent that stimulates the contraction of gastric fundus in dog. As synthetic porcine motilin or its analogues had become available, its biological actions on the gastrointestinal tract were investigated by various groups of workers.…”

“…Motilin, given intravenously, stimulates contraction of the canine stomach (Brown et al, 1971;Jennewein et al, 1975 ;Lee et al, 1974), lower esophageal sphincter (Jennewein et al, 1975 ;Gutierrez et al, 1977, Meissner et al, 1976, and small intestine (Jennewein et al, 1975 ;Lee et al, 1974). Motilin was also shown to increase the number of spike potentials of myoelectric activity in the canine antrum and small intestine (Castresana et al, 1978 ;Wingate et al, 1976).…”