The functional indexes of RBCs and microcirculation in the traumatic brain injury with the action of 2-ethil-6-methil-3-hydroxypiridin succinate

Abstract

Research aim To study the RBCs functional and metabolic parameters and the microcirculatory brain structure at traumatic brain injury (TBI) under the action of 2-ethyl-6-methyl-3-hydroxypyridine succinate. Methods A closed TBI was modeled by the free fall of a load on the parietooccipital regions of head. We made studies of the influence of 2-ethil-6-methil-3-hydroxipiridin succinate on aggregation and electrophoretic mobility of RBCs, catalase activity, malonic dialdehyde concentration, adenosine triphosphate and 2.3-biphosphoglycerate (2.3 – BPG) concentrations in RBCs. The state of parenchyma and microcirculatory brain mainstream in post-traumatic period of TBI have been studied on micro-preparations. Results The use of 2-ethyl-6-methyl-3-hydroxypyridine succinate under conditions of head injury leads to a decrease in MDA concentration and in aggregation of RBCs, to an increase in the 2.3—BPG concentration and RBC electrophoretic mobility compared to the control (group value). The most pronounced changes under the action of 2-ethyl-6-methyl-3-hydroxypyridine succinate were observed 3–7 days after the TBI. Significant indicators of the restoration of the microvasculature and brain tissue provoked by the use of 2-ethyl-6-methyl-3-hydroxypyridine succinate of were evident from the 7th day unlike the control group, where the restoration of structural morphological parameters was observed only on the 12th day of the post-traumatic period. Fast recovery of blood flow under the action of 2-ethyl-6-methyl-3-hydroxypyridine succinate ensured effective restoration of neurons and glia in comparison with the control group. Conclusions Early and long-term cytoprotective correction intensifies the oxygen transport function of the blood, prevents and / or reduces disorders of microvessels, neurons and glia in the post-traumatic period, thereby provides correction of hypoxic state and drives to the restoration of brain tissues homeostasis.

Функціональні Й Метаболічні Порушення Нирок За Умов Скелетної Травми, Ускладненої Гострою Крововтратою Різного Ступеня Тяжкості, Та Ефективність 2-Етил-6-Метил-3-Гідроксипіридину Сукцинату В Корекції Виявлених Порушень

“…Усе це змушує вести пошук додаткових засобів протекції тканин від гіпоксичного впливу й оксидативного стресу, які належать до ключових патогенних чинників при травмі та гострій крововтраті. Останнім часом серед комплексних засобів антиоксидантної та антигіпоксантної дії широко використовують 2етил6метил3гідроксипіридину сукцинат [14,15]. Препарат поліпшує енергетичний статус клітин, обмежує оксидативний стрес, стабілізує клітинні мембрани.…”