The functional dlt operon of Clostridium butyricum controls the d-alanylation of cell wall components and influences cell septation and vancomycin-induced lysis

Wydau-Dematteis, Sandra; Louis, Mathilde; Zahr, Noël; Laï-Kuen, René; Saubaméa, Bruno; Butel, Marie‐José; Pons, Jean-Louis

doi:10.1016/j.anaerobe.2015.09.001

Cited by 13 publications

(6 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…butyricum CB1002 (Wydau-Dematteis et al, 2015). Although the Clos-Tron technique has proven to have capacity to deliver heterologous genes, the size of cargo sequence would negatively affect the targeting efficiency (Heap et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…However, there are relatively few studies focusing on metabolic engineering of C. butyricum compared to other Clostridium strains, such as C. acetobutylicum. Until now, the ClosTron (or TargeTron) system is the only available genetic tool that can conduct chromosomal gene knockout in C. butyricum (Cai et al, 2011(Cai et al, , 2013Wydau-Dematteis et al, 2015). The clostridial ClosTron system was established in 2007 based on the Group II intron technology and has been broadly applied to various clostridial species (Heap et al, 2007;Pyne et al, 2014).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Exploiting heterologous and endogenous CRISPR‐Cas systems for genome editing in the probiotic Clostridium butyricum

Zhou

Wang

Luo

et al. 2021

Biotech & Bioengineering

View full text Add to dashboard Cite

Clostridium butyricum has been widely used as a probiotic for humans and food animals. However, the mechanisms of beneficial effects of C. butyricum on the host remain poorly understood, largely due to the lack of high-throughput genome engineering tools. Here, we report the exploitation of heterologous Type II CRISPR-Cas9 system and endogenous Type I-B CRISPR-Cas system in probiotic C. butyricum for seamless genome engineering. Although successful genome editing was achieved in C. butyricum when CRISPR-Cas9 system was employed, the expression of toxic cas9 gene result in really poor transformation, spurring us to develop an easyapplicable and high-efficient genome editing tool. Therefore, the endogenous Type I-B CRISPR-Cas machinery located on the megaplasmid of C. butyricum was coopted for genome editing. In vivo plasmid interference assays identified that ACA and TAA were functional protospacer adjacent motif sequences needed for sitespecific CRISPR attacking. Using the customized endogenous CRISPR-Cas system, we successfully deleted spo0A and aldh genes in C. butyricum, yielding an efficiency of up to 100%. Moreover, the conjugation efficiency of endogenous CRISPR-Cas system was dramatically enhanced due to the precluding expression of cas9. Altogether, the two approaches developed herein remarkably expand the existing genetic toolbox available for investigation of C. butyricum.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Exploiting heterologous and endogenous CRISPR‐Cas systems for genome editing in the probiotic Clostridium butyricum

Zhou

Wang

Luo

et al. 2021

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

“…butyricum associated NEC by establishing the resistance of C . butyricum to antimicrobial peptides, lysozymes and vancomycin 24 . A high dose of butyric acid has also been demonstrated to have a cytotoxic effect on diverse cell lines, notably Caco-2 cells monolayers 25 .…”

Section: Discussionmentioning

confidence: 99%

Multidisciplinary evaluation of Clostridium butyricum clonality isolated from preterm neonates with necrotizing enterocolitis in South France between 2009 and 2017

Hosny

Khalil

Caputo

et al. 2019

Sci Rep

View full text Add to dashboard Cite

The association between Clostridium species identification from stool samples in preterm neonates and the occurrence of necrotizing enterocolitis has been increasingly reported. To confirm the specific impact of Clostridium butyricum in this pathology, selective culture procedure was used for Clostridia isolation. Whole-genome analysis was employed to investigate genomic relationships between isolates. Stool samples from present study, as well as from previously investigated cases, were implicated including 88 from preterm neonates with necrotizing enterocolitis and 71 from matched controls. Quantitative real-time polymerase chain reaction was performed to evaluate the presence of C. butyricum from stools of new cases. Clostridium species prevalence isolated by culture was compared between patients with necrotizing enterocolitis and controls. By combining results of both culture and quantitative polymerase chain reaction methods, C. butyricum was significantly more frequent in stool samples from preterm neonates with necrotizing enterocolitis than in controls. Whole-genome analysis of 81 genomes including 58 neonates’ isolates revealed that cases were clustered depending on geographical origin of isolation. Controls isolates presented genomic relations with that of patients suggesting a mechanism of asymptomatic carriage. Overall, this suggests an epidemiology comparable to that observed in Clostridium difficile colitis in adults.

show abstract

“…Staphylococcus Enterococcus GAS GBS Pneumococci D-alanylation of the membrane X X X X X Peschel et al, 1999;Poyart et al, 2001Poyart et al, , 2003Abachin et al, 2002;Frick et al, 2003;Cao and Helmann, 2004;Kristian et al, 2005;May et al, 2005;Fabretti et al, 2006;Fisher et al, 2006;Kovacs et al, 2006;Palumbo et al, 2006;Walter et al, 2007;Beiter et al, 2008;Abi Khattar et al, 2009;Cox et al, 2009;Jann et al, 2009;McBride and Sonenshein, 2011a;Saar-Dover et al, 2012;Simanski et al, 2013;Carvalho et al, 2015;Wydau-Dematteis et al, 2015;Kamar et al, 2017;Hirt et al, 2018 Lysinylation of the membrane X X X Oku et al, 2004;Staubitz et al, 2004;Kraus and Peschel, 2006;Thedieck et al, 2006;Ernst et al, 2009;Samant et al, 2009;Bao et al, 2012;Shireen et al, 2013;Kumariya et al, 2015;Nasser et al, 2019 O-acetylation of the peptidoglycan X X Crisostomo et al, 2006;Pfeffer et al, 2006;Hebert et al, 2007;Aubry et al, 2011;Laaberki et al, 2011;…”

Section: Gram-positive Bacillimentioning

confidence: 99%

Resistance Mechanisms to Antimicrobial Peptides in Gram-Positive Bacteria

et al. 2020

View full text Add to dashboard Cite

With the alarming increase of infections caused by pathogenic multidrug-resistant bacteria over the last decades, antimicrobial peptides (AMPs) have been investigated as a potential treatment for those infections, directly through their lytic effect or indirectly, due to their ability to modulate the immune system. There are still concerns regarding the use of such molecules in the treatment of infections, such as cell toxicity and host factors that lead to peptide inhibition. To overcome these limitations, different approaches like peptide modification to reduce toxicity and peptide combinations to improve therapeutic efficacy are being tested. Human defense peptides consist of an important part of the innate immune system, against a myriad of potential aggressors, which have in turn developed different ways to overcome the AMPs microbicidal activities. Since the antimicrobial activity of AMPs vary between Gram-positive and Gram-negative species, so do the bacterial resistance arsenal. This review discusses the mechanisms exploited by Gram-positive bacteria to circumvent killing by antimicrobial peptides. Specifically, the most clinically relevant genera, Streptococcus spp., Staphylococcus spp., Enterococcus spp. and Gram-positive bacilli, have been explored.

show abstract

The functional dlt operon of Clostridium butyricum controls the d-alanylation of cell wall components and influences cell septation and vancomycin-induced lysis

Cited by 13 publications

References 54 publications

Exploiting heterologous and endogenous CRISPR‐Cas systems for genome editing in the probiotic Clostridium butyricum

Exploiting heterologous and endogenous CRISPR‐Cas systems for genome editing in the probiotic Clostridium butyricum

Multidisciplinary evaluation of Clostridium butyricum clonality isolated from preterm neonates with necrotizing enterocolitis in South France between 2009 and 2017

Resistance Mechanisms to Antimicrobial Peptides in Gram-Positive Bacteria

Contact Info

Product

Resources

About