The Function of Heat-Shock Proteins in Stress Tolerance: Degradation and Reactivation of Damaged Proteins

Parsell, Dawn A.; Lindquist, Susan

doi:10.1146/annurev.ge.27.120193.002253

Cited by 2,023 publications

(909 citation statements)

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“…1978; Kelley and Schlesinger 1982; Kregel 2002; Chang 2009). In spite of little difference in the main functions among the different families, HSPs have been suggested to act as auxiliary proteins to assist with correct folding, assembly and disassembly, and transport and positioning between organelles (Lindquist and Craig 1988; Parsell and Lindquist 1993; Young et al. 2004).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome response to temperature stress in the wolf spider Pardosa pseudoannulata (Araneae: Lycosidae)

Xiao

Wang

Cao

et al. 2016

Ecology and Evolution

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The wolf spider Pardosa pseudoannulata is a dominant predator in paddy ecosystem and an important biological control agent of rice pests. Temperature represents a primary factor influencing its biology and behavior, although the underlying molecular mechanisms remain unknown. To understand the response of P. pseudoannulata to temperature stress, we performed comparative transcriptome analyses of spider adults exposed to 10°C and 40°C for 12 h. We obtained 67,725 assembled unigenes, 21,765 of which were annotated in P. pseudoannulata transcriptome libraries, and identified 905 and 834 genes significantly up‐ or down‐regulated by temperature stress. Functional categorization revealed the differential regulation of transcription, signal transduction, and metabolism processes. Calcium signaling pathway and metabolic pathway involving respiratory chain components played important roles in adapting to low temperature, whereas at high temperature, oxidative phosphorylation and amino acid metabolism were critical. Differentially expressed ribosomal protein genes contributed to temperature stress adaptation, and heat shock genes were significantly up‐regulated. This study represents the first report of transcriptome identification related to the Araneae species in response to temperature stress. These results will greatly facilitate our understanding of the physiological and biochemical mechanisms of spiders in response to temperature stress.

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Section: Discussionmentioning

confidence: 99%

Transcriptome response to temperature stress in the wolf spider Pardosa pseudoannulata (Araneae: Lycosidae)

Xiao

Wang

Cao

et al. 2016

Ecology and Evolution

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“…This indicates that under these conditions the increased abundance of Hsp27 is mainly due to an increased synthesis rate. Along with the up-regulation of proteins needed for the cell to survive, heat stress also leads to down-regulation of other proteins [12]. While the total concentration of actin had decreased by approximately 20% at t 36 (Fig.…”

Section: Detection Of Changes In Protein Synthesis and Degradation Ramentioning

confidence: 97%

A proteomic method for the analysis of changes in protein concentrations in response to systemic perturbations using metabolic incorporation of stable isotopes and mass spectrometry

et al. 2005

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While several techniques exist for assessing quantitative differences among proteomes representing different cell states, methods for assessing how these differences are mediated are largely missing. We present a method that allows one to differentiate between cellular processes, such as protein synthesis, degradation and PTMs which affect protein concentrations. An induced systemic perturbation of a cell culture was coupled to a replacement of the growth medium to one highly enriched in the stable isotope 15 N. The relative abundance of the 15 N-and 14 N-enriched forms of proteins, isolated from cell cultures harvested at time points following the onset of the perturbation, were determined by MS. Alterations in protein synthesis and degradation were quantified by comparing proteins isolated from perturbed and unperturbed cultures, respectively. The method was evaluated by subjecting HeLa cells to heat stress. As expected, a number of known heat shock proteins (Hsp) increased in concentration during heat stress. For Hsp27, increased de novo synthesis accounted for the concentration increase, while for Hsp70, decreased degradation accounted for the increase. A protein that was detected only after prolonged heat stress, vimentin, was not primarily synthesized de novo, but appeared rather as a result of PTM.

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“…The result is unfolding and aggregation of various cellular proteins [ 7 ], activation of the stress genes [ 8 ] and heat shock proteins (Hsps) synthesis. The concentration of proteins, products of these genes, is increased in response to stressors, thus protecting the cells from the damaging effects of stress and accelerating recovery by helping renaturation of partially-denaturated proteins [ 9 ].…”

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confidence: 99%

Evaluation of the Expression of the Low Weight Heat Shock Protein αB-crystallin in Pulmonary Neuroendocrine Tumours

Marinova¹,

Slavova²,

Trifonova³

et al. 2013

Proceeding BAS

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αB-crystallin is a major heat-inducible small heat shock protein, which is supposed to be implicated in tumour cell proliferation, differentiation, invasion, metastasis and apoptosis. Still the expression pattern of the phosphorylated on serin 59 form of αB-crystallin in lung cancer is not yet investigated.The aim of the present work is to study this pattern in different histological subtypes of pulmonary neuroendocrine tumours (pNETs).Surgically resected specimens from 143 patients with pNETs were studied. The histological subtype, pathological Tumour-Node-Metastasis stage and the immunohistochemical expression of phosphorylated αB-crystallin were evaluated.We demonstrated that the nucleus was the major localization site of the protein although there were some rare cases showing also diffuse cytoplasmic expression. High grade pNETs (small cell lung carcinoma and large cell neuroendocrine carcinoma) showed nuclear expression in all cases but most frequently it was in less than 50% of the tumour cells; cytoplasmic expression was shown in single cases. Just the opposite was observed in low and intermediate grade pNETs (typical carcinoids and atypical carcinoids) -nuclear expression was not seen in all of the cases, but when it was present it was in almost all tumour cells; the cases expressing phosphorylated αB-crystallin also in the cytoplasm were mainly typical carcinoids. We demonstrated that cytoplasmic 281 expression was associated with earlier stage and absence of metastases, which may determine it as a potential marker for a favourable prognosis in patients.We conclude that phosphorylated αB-crystallin probably plays a major role in pNETs biology, it may be associated with tumour grade, it may have a prognostic value, and it may be used as future target for therapy.Key words: heat shock proteins, αB-crystallin, pulmonary neuroendocrine tumours 1. Introduction. The lung cancer clinical course is determined by its histological and biological features [ 1 ]. The prognosis in lung cancer patients is associated with certain clinicopathological features as histological type, tumour size and invasion of adjacent structures (T-status), lymph node metastases (N-status) and pTNM (pathological Tumour-Node-Metastasis) stage [ 2, 3 ]. These features are not always sufficient for complete and objective assessment of lung tumours, which requires in-depth studies of immunohistochemical and genetic indicators that could serve as better markers for determining the biological behaviour of these tumours [ 4 ].Adenocarcinoma and squamous cell carcinoma are the most common lung tumours (85% of all lung cancers) and thus they are the most actively studied group. A more special and rare group are the pulmonary neuroendocrine tumours (pNETs) comprising very different tumours regarding biological behaviour and prognosis -typical carcinoids (TCs), atypical carcinoids (AtCs), small cell lung carcinomas (SCLCs) and large-cell neuroendocrine carcinomas (LCNECs) Various factors (such as hyperthermia, hypoxia, heavy metals, ethan...

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The Function of Heat-Shock Proteins in Stress Tolerance: Degradation and Reactivation of Damaged Proteins

Cited by 2,023 publications

References 271 publications

Transcriptome response to temperature stress in the wolf spider Pardosa pseudoannulata (Araneae: Lycosidae)

Transcriptome response to temperature stress in the wolf spider Pardosa pseudoannulata (Araneae: Lycosidae)

A proteomic method for the analysis of changes in protein concentrations in response to systemic perturbations using metabolic incorporation of stable isotopes and mass spectrometry

Evaluation of the Expression of the Low Weight Heat Shock Protein αB-crystallin in Pulmonary Neuroendocrine Tumours

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