The French press: a repeatable and high-throughput approach to exercising zebrafish (<i>Danio rerio</i>)

Usui, Takuji; Noble, Daniel W. A.; Fangmeier, Melissa L; Lagisz, Malgorzata; Hesselson, Daniel; Nakagawa, Shinichi

doi:10.7717/peerj.4292

Cited by 6 publications

(5 citation statements)

References 30 publications

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“…Both were broadly accepted by zebrafish and efficient in increasing the maximum speed after 2 and 8 weeks of training, respectively. Similar results of increasing maximum speed by exercising of zebrafish were recently reported [20]. Additionally, we found a sexspecific difference with males having greater maximum speed than female zebrafish.…”

Section: Discussionsupporting

confidence: 91%

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Metabolic and Transcriptional Adaptations Improve Physical Performance of Zebrafish

et al. 2021

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Obesity is a worldwide public health problem with increasing prevalence and affects 80% of diabetes mellitus type 2 cases. Zebrafish (Danio rerio) is an established model organism for studying obesity and diabetes including diabetic microvascular complications. We aimed to determine whether physical activity is an appropriate tool to examine training effects in zebrafish and to analyse metabolic and transcriptional processes in trained zebrafish. A 2- and 8-week experimental training phase protocol with adult zebrafish in a swim tunnel system was established. We examined zebrafish basic characteristics before and after training such as body weight, body length and maximum speed and considered overfeeding as an additional parameter in the 8-weeks training protocol. Ultimately, the effects of training and overfeeding on blood glucose, muscle core metabolism and liver gene expression using RNA-Seq were investigated. Zebrafish maximum speed was correlated with body length and was significantly increased after 2 weeks of training. Maximum swim speed further increased after 8 weeks of training in both the normal-fed and the overfed groups, but training was found not to be sufficient in preventing weight gain in overfed fish. Metabolome and transcriptome profiling in trained fish exhibited increased blood glucose levels in the short-term and upregulated energy supply pathways as well as response to oxidative stress in the long-term. In conclusion, swim training is a valuable tool to study the effects of physical activity in zebrafish, which is accompanied by metabolic and transcriptional adaptations.

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Section: Discussionsupporting

confidence: 91%

“…The swim tunnel is based on a propeller, which generates a water flow in a swim tube, forcing the zebrafish to swim against the current. However, the effects of physical performance on physiological parameters and the zebrafish metabolism and transcriptome have rarely been studied so far [19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Metabolic and Transcriptional Adaptations Improve Physical Performance of Zebrafish

et al. 2021

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“…Alterations in expression of cardiac and skeletal muscle genes after exercise were mostly studied in fish subjected to linear tunnel swimming [ 49 – 51 , 70 , 71 ] and their transcriptomic response to endurance exercise was determined by microarray analysis [ 49 , 51 ]. The exercise method used in this study is similar to published set-ups with varying swimming conditions, employed to study motor coordination [ 40 ] and swim performance [ 72 ]. To demonstrate that our swimming protocol caused the activation of striated muscle in adult zebrafish, we analyzed the expression of selected exercise responsive genes from different functional categories: muscle growth and development, muscle contraction, extracellular matrix, protein synthesis and degradation, metabolism, and myokines [ 49 – 51 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of zebrafish (Danio rerio) muscle ankyrin repeat proteins reveals their conserved response to endurance exercise

et al. 2018

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Muscle proteins with ankyrin repeats (MARPs) ANKRD1 and ANKRD2 are titin-associated proteins with a putative role as transcriptional co-regulators in striated muscle, involved in the cellular response to mechanical, oxidative and metabolic stress. Since many aspects of the biology of MARPs, particularly exact mechanisms of their action, in striated muscle are still elusive, research in this field will benefit from novel animal model system. Here we investigated the MARPs found in zebrafish for protein structure, evolutionary conservation, spatiotemporal expression profiles and response to increased muscle activity. Ankrd1 and Ankrd2 show overall moderate conservation at the protein level, more pronounced in the region of ankyrin repeats, motifs indispensable for their function. The two zebrafish genes, ankrd1a and ankrd1b, counterparts of mammalian ANKRD1/Ankrd1, have different expression profiles during first seven days of development. Mild increase of ankrd1a transcript levels was detected at 72 hpf (1.74±0.24 fold increase relative to 24 hpf time point), while ankrd1b expression was markedly upregulated from 24 hpf onward and peaked at 72 hpf (92.18±36.95 fold increase relative to 24 hpf time point). Spatially, they exhibited non-overlapping expression patterns during skeletal muscle development in trunk (ankrd1a) and tail (ankrd1b) somites. Expression of ankrd2 was barely detectable. Zebrafish MARPs, expressed at a relatively low level in adult striated muscle, were found to be responsive to endurance exercise training consisting of two bouts of 3 hours of forced swimming daily, for five consecutive days. Three hours after the last exercise bout, ankrd1a expression increased in cardiac muscle (6.19±5.05 fold change), while ankrd1b and ankrd2 were upregulated in skeletal muscle (1.97±1.05 and 1.84±0.58 fold change, respectively). This study provides the foundation to establish zebrafish as a novel in vivo model for further investigation of MARPs function in striated muscle.

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“…Such a characteristic is highly advantageous, providing the potential for exercise interventions studied in aged zebrafish to be of translational value when developing programs for aging humans. Various cost-effective, benchtop apparatuses to study exercise in zebrafish have been developed [ 58 , 59 , 60 ]. Such systems include the ‘French press’, a high-throughput approach that entails the use of a coffee plunger and magnetic stirrer to produce water currents, enabling zebrafish to be exercised and trained [ 58 ].…”

mentioning

confidence: 99%

“…Various cost-effective, benchtop apparatuses to study exercise in zebrafish have been developed [ 58 , 59 , 60 ]. Such systems include the ‘French press’, a high-throughput approach that entails the use of a coffee plunger and magnetic stirrer to produce water currents, enabling zebrafish to be exercised and trained [ 58 ]. Exercise-induced changes to bone can also be studied using a controlled swimming program such as the one developed by Suniaga et al Zebrafish were placed to swim against laminar currents in swimming chambers, and zebrafish displayed bone adaptation in response to musculoskeletal exercise [ 54 ].…”

mentioning

confidence: 99%

Commentary: Zebrafish as a Model for Osteoporosis—An Approach to Accelerating Progress in Drug and Exercise-Based Treatment

Dubale

Kapron

West

2022

IJERPH

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Osteoporosis (OP) is a degenerative disease characterized by reduced bone strength and increased fracture risk. As the global population continues to age, the prevalence and economic burden of osteoporosis can be expected to rise substantially, but there remain various gaps in the field of OP care. For instance, there is a lack of anti-fracture drugs with proven long-term efficacy. Likewise, though exercise remains widely recommended in OP prevention and management, data regarding the safety and efficacy for patients after vertebral fracture remain limited. This lack of evidence may be due to the cost and inherent difficulties associated with exercise-based OP research. Thus, the current research landscape highlights the need for novel research strategies that accelerate OP drug discovery and allow for the low-cost study of exercise interventions. Here, we outline an example of one strategy, the use of zebrafish, which has emerged as a potential model for the discovery of anti-osteoporosis therapeutics and study of exercise interventions. The strengths, limitations, and potential applications of zebrafish in OP research will be outlined.

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The French press: a repeatable and high-throughput approach to exercising zebrafish (Danio rerio)

Cited by 6 publications

References 30 publications

Metabolic and Transcriptional Adaptations Improve Physical Performance of Zebrafish

Metabolic and Transcriptional Adaptations Improve Physical Performance of Zebrafish

Characterization of zebrafish (Danio rerio) muscle ankyrin repeat proteins reveals their conserved response to endurance exercise

Commentary: Zebrafish as a Model for Osteoporosis—An Approach to Accelerating Progress in Drug and Exercise-Based Treatment

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