The Fractional Precipitation of Antitoxic Serum

Banzhaf, Edwin J.; Gibson, Robert B.

doi:10.1016/s0021-9258(17)45981-3

Cited by 4 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The resulting mAbs were generated by inoculating the selected hybridoma cells into Balb/c mice treated with mineral oil. Antiartemisinin mAbs were purified from ascite fluids by ammonium sulfate precipitation …”

Section: Materials and Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Development of a Specific Monoclonal Antibody for the Quantification of Artemisinin in Artemisia annua and Rat Serum

et al. 2016

View full text Add to dashboard Cite

Artemisinin, extracted from Artemisia annua, and its derivatives are important frontline antimalarials. To produce specific antibodies for the detection and quantification of artemisinin, artemisinin was transformed to 9-hydroxyartemisinin by microbial fermentation, which was used to prepare a 9-succinate artemisinin hapten for conjugation with ovalbumin. A monoclonal antibody (mAb), designated as 3H7A10, was selected from hybridoma cell lines which showed high specificity to artemisinin. No competitive inhibition was observed with artesunate, dihydroartemisinin, and artemether for up to 20,000 ng mL−1. An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed, which showed a concentration causing 50% of inhibition (IC50) for artemisinin as 2.6 ng mL−1 and a working range of 0.6–11.5 ng mL−1. The icELISA was applied for the quantification of artemisinin in crude extracts of wild A. annua and the study of pharmacokinetics of artemisinin in rat serum after intraperitoneal injection. The results were highly correlated with those determined by HPLC-UV analysis (R2=0.9919). In comparison with reported anti-artemisinin mAbs which have broad cross reactivity with other artemisinin derivatives, the high specificity of 3H7A10 for artemisinin will enable development of methods for quantification of artemisinin in Artemisia plants and antimalarial drugs such as Arco®, and for pharmacokinetic studies.

show abstract

Section: Materials and Methodsmentioning

confidence: 99%

“…Antiartemisinin mAbs were purified from ascite fluids by ammonium sulfate precipitation. 22 Indirect Competitive ELISA (icELISA) and Indirect ELISA (iELISA). The protocol for icELISA and iELISA was the same as that described previously.…”

Section: Methodsmentioning

confidence: 99%