The Formation of Polycomplexes of Poly(Methyl Vinyl Ether-Co-Maleic Anhydride) and Bovine Serum Albumin in the Presence of Copper Ions

Abstract

The binary and ternary complex formations of poly(methyl vinyl ether-co-maleic anhydride) (PMVEMA) with copper ions and with bovine serum albumin (BSA) in the presence of copper ions in phosphate buffer solution at pH = 7 were examined by the techniques of UV-visible, fl uorescence, dynamic light scattering, atomic force microscopy measurements. In the formation of binary complexes of PMVEMA-Cu(II), the addition of copper ions to the solution of PMVEMA in phosphate buffer solution at pH = 7 forms homogeneous solutions when the molar ratio of Cu(II)/MVEMA is 0.5. Then the formations of ternary complexes of PMVEMA-Cu(II)-BSA were examined. Study analysis revealed that the toxicities of polymer-metal and polymer-metal-protein mixture solutions depend on the nature and ratio of components in mixtures.

Towards C1 chemistry: methanol vinylation by CaC2 in water in the presence of potassium or sodium carbonates

“…Copolymers of MVE with maleic anhydride are widely used in medicine and pharmacology, in paper and textile industries, for manufacture of cosmetics and toiletry, as bioadhesives for drug delivery options and components of cellulosic biocomposites . Besides, these copolymers form interpolymer complexes possessing valuable properties …”

“…35,36 Besides, these copolymers form interpolymer complexes possessing valuable properties. 37 Industrially (BASF, GAF, Union Carbide), MVE is synthesized by vinylation of methanol with acetylene under elevated pressure (10-18 atm) at 120-160 ∘ C in the presence of alkali metal hydroxides or their alcoholates. [6][7][8] Furthermore, in acetylene chemistry a trend to replace free acetylene by CaC 2 , 17 particularly in the www.soci.org LN Parshina et al synthesis of vinyl ethers, [16][17][18][19][20][21][22] is gaining strength.…”

New Generation Peptide-Based Vaccine Prototype

“…Different approaches are available to develop synthetic peptide-based vaccines, using metal ions in combination with peptide sequences. In particular, the investigation of the complex formation biopolymer by peptide in the presence of metal ions contributes greatly to the technological development of peptide-based vaccine prototypes [19]. The contact of the peptides with the polyelectrolyte (PE) is found at the interface.…”

“…The hydrophobic interactions in such a complex create an adjuvant effect for prototyping technology in vaccination. [19][20][21][22]. In the studies on the development of peptide vaccine prototypes previously made by our study group, it was observed that the purification of characterization of binding of synthetic peptides to various adjuvants and subsequent high immune response was obtained in BALB/c mice from experimental animals [23].…”

“…The fact that this change can be made only by living cells in vitro has made tetrazolium compounds a highly biologically important to measure toxicity of peptide-based vaccine formulas. The mechanism of toxicity assays, such as 3-(4,5-dimethylthiazolyl)-2,5 diphenyltetrazolium bromide (MTT) [19] [49,50] and in our studies, we generally use MTT analysis. For example, our technological vaccine prototype example is Zika peptide loaded PLGA nanoparticles which were determined on ECV304 human epithelial cells via MTT assay, which is the cytotoxicity test, was performed to determine the cytotoxic effects of the peptide, peptide loaded NPs [45].…”